Abstract
The experiments of Arabidopsis thaliana suspension culture were performed. Some subculture conditions were determined, and the growth characteristics of suspension cells were observed. The typical S-like growth curve in a subculture cycle lasting 7 days was obtained. In normal condition, a logarithmic phase of growth of suspension biomass occurred in 2-4 days after subculture. Then, the growth of biomass slowed down quickly. However, its growth and time of logarithmic phase were changed with amount of inoculation. It was advisable to choose 10 mL/50 mL as diluting ratio for holding subculture cycle of 7 days. The growth was slowed down, and even was halted with 10 mL/50 mL as diluting ratio. Different concentration of hormones and sucrose in media greatly affected growth of suspension cells. NAA could restrain growth of suspension cells subcultured in B5 + 0. 2 mg/L NAA medium for several times. 30 g/L sucrose was advisable, and lower concentration of sucrose could decrease biomass and activity of suspension cells.
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Claims 11
Claim · model-written summary
Arabidopsis suspension biomass followed an S-like growth curve over a seven-day subculture cycle.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Outcome
- S-like growth curvee1
- Value
- 7 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“The typical S-like growth curve in a subculture cycle lasting 7 days was obtained.”
Claim · model-written summary
Suspension biomass entered logarithmic growth two to four days after subculture.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- logarithmic phase of growth of suspension biomasse1
- Value
- 2-4 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“a logarithmic phase of growth of suspension biomass occurred in 2-4 days after subculture”
Claim · model-written summary
Biomass growth then slowed quickly.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- growth of biomasse1
- Direction
- slowed downe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“Then, the growth of biomass slowed down quickly.”
Claim · model-written summary
Inoculation amount changed suspension-cell growth and the timing of its logarithmic phase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- amount of inoculatione1
- Outcome
- growth and time of logarithmic phasee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“its growth and time of logarithmic phase were changed with amount of inoculation”
Claim · model-written summary
A 10 mL/50 mL dilution ratio was recommended for a seven-day subculture cycle.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- choose 10 mL/50 mL as diluting ratio for holding subculture cycle of 7 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“It was advisable to choose 10 mL/50 mL as diluting ratio for holding subculture cycle of 7 days.”
Claim · model-written summary
At a 10 mL/50 mL dilution ratio, suspension-cell growth slowed or stopped.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- 10 mL/50 mL as diluting ratioe1
- Outcome
- growthe1
- Direction
- slowed downe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“The growth was slowed down, and even was halted with 10 mL/50 mL as diluting ratio.”
Claim · model-written summary
Hormone concentration strongly affected suspension-cell growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Different concentration of hormonese1
- Outcome
- growth of suspension cellse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“Different concentration of hormones and sucrose in media greatly affected growth of suspension cells.”
Claim · model-written summary
Sucrose concentration strongly affected suspension-cell growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- sucrose in mediae1
- Outcome
- growth of suspension cellse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“Different concentration of hormones and sucrose in media greatly affected growth of suspension cells.”
Claim · model-written summary
NAA restrained suspension-cell growth after repeated subculture in B5 plus NAA medium.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- B5 + 0. 2 mg/L NAA mediume1
- Outcome
- growth of suspension cellse1
- Direction
- restraine1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“NAA could restrain growth of suspension cells subcultured in B5 + 0. 2 mg/L NAA medium for several times.”
Claim · model-written summary
The authors recommended 30 g/L sucrose for suspension cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- 30 g/L sucrose was advisablee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“30 g/L sucrose was advisable”
Claim · model-written summary
Lower sucrose concentration decreased suspension-cell biomass and activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- lower concentration of sucrosee1
- Outcome
- biomass and activity of suspension cellse1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The experiments of Arabidopsis thaliana suspension culture were performed.”
e1“lower concentration of sucrose could decrease biomass and activity of suspension cells.”
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