Paper · 2008 · Guoshu xuebao

Effects of different AgNO_3 concentrations on shoot regeneration and activities of antioxidant enzymes in in vitro leaves of pear

Ling‐Mei Liu

11
claims
22
verbatim quotes
1
citations
3
research areas
—
Not open access

Abstract

Effects of different AgNO3 concentrations on the regeneration frequency of in vitro leaves and the activities of antioxidant enzymes in Jinshui and Jinhua pear cultivars (Pyrus brestchneideri Rehd.) were studied. The shoot regeneration frequency and buds per leaves of Jinshui cultivar dealt with 0.1 mg/L AgNO3 were improved significantly. The shoot regeneration frequency from leaves of Jinhua cultivar was enhanced on medium contained 0.1 to 0.5 mg/L AgNO3. The activities of antioxidant enzymes indicated significantly different compared with the control when the leaves dealt with AgNO3. During the period of dark treatment, CAT and POD activities in leaves were changed heavily, especially the changes of POD activity in the leaves of Jinhua cultivar and the CAT activity in the leaves of Jinshui cultivar was higher than the other three treatments when they dealt with 0.1 mg/L AgNO3. At the same time, production rates and H2O2 content would increase but the ascending degree was lower than the control. At the stage of light treatment, the pinnacle of SOD would enhance when adding apt concentration of AgNO3, while H2O2 content and production rates would decrease. On the contrary, higher concentration of AgNO3 would cause H2O2 content and production rates rising again. Though the changes of antioxidant enzymes in different cultivars were not the same,it could be concluded that 0.1 mg/L AgNO3 was the best to increase the activity of antioxidant enzymes in the leaves of both Jjinshui and Jinhua pear cultivars among the 4 treatments.

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Claims 11

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

At 0.1 mg/L AgNO3, Jinshui shoot regeneration frequency and buds per leaf improved significantly.

Material
Jinshuie0
Treatment
0.1 mg/L AgNO3e0
Outcome
shoot regeneration frequency and buds per leavese0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The shoot regeneration frequency and buds per leaves of Jinshui cultivar dealt with 0.1 mg/L AgNO3 were improved significantly.”

✓ located verbatim in the source · abstract, characters 213–340 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Jinhua leaf shoot regeneration was enhanced at 0.1 to 0.5 mg/L AgNO3.

Material
Jinhuae0
Treatment
0.1 to 0.5 mg/L AgNO3e0
Outcome
shoot regeneration frequency from leavese0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The shoot regeneration frequency from leaves of Jinhua cultivar was enhanced on medium contained 0.1 to 0.5 mg/L AgNO3.”

✓ located verbatim in the source · abstract, characters 341–460 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

AgNO3 significantly changed pear leaf antioxidant enzyme activities relative to controls.

Treatment
AgNO3e0
Outcome
activities of antioxidant enzymese0
Direction
differente0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The activities of antioxidant enzymes indicated significantly different compared with the control when the leaves dealt with AgNO3.”

✓ located verbatim in the source · abstract, characters 461–592 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

During dark treatment, pear leaf CAT and POD activities changed substantially.

Treatment
dark treatmente0
Outcome
CAT and POD activities in leavese0
Direction
changed heavilye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“During the period of dark treatment, CAT and POD activities in leaves were changed heavily”

✓ located verbatim in the source · abstract, characters 593–683 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

At 0.1 mg/L AgNO3 in darkness, Jinhua POD activity showed a marked change.

Material
Jinhua cultivare0
Treatment
0.1 mg/L AgNO3e0
Outcome
POD activitye0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“especially the changes of POD activity in the leaves of Jinhua cultivar and the CAT activity in the leaves of Jinshui cultivar was higher than the other three treatments when they dealt with 0.1 mg/L AgNO3.”

✓ located verbatim in the source · abstract, characters 685–891 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 0.1 mg/L AgNO3 in darkness, Jinshui CAT activity exceeded the other three treatments.

Material
Jinshui cultivare0
Treatment
0.1 mg/L AgNO3e0
Outcome
CAT activitye0
Direction
highere0
Comparator
other three treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the CAT activity in the leaves of Jinshui cultivar was higher than the other three treatments when they dealt with 0.1 mg/L AgNO3.”

✓ located verbatim in the source · abstract, characters 761–891 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Production rates and H2O2 content increased less than in controls during dark treatment.

Outcome
production rates and H2O2 contente0
Direction
increasee0
Comparator
the controle0

Not reportedTreatment

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“production rates and H2O2 content would increase but the ascending degree was lower than the control.”

✓ located verbatim in the source · abstract, characters 910–1011 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

During light treatment, suitable AgNO3 concentrations enhanced the SOD peak.

Treatment
apt concentration of AgNO3e0
Outcome
pinnacle of SODe0
Direction
enhancee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“At the stage of light treatment, the pinnacle of SOD would enhance when adding apt concentration of AgNO3”

✓ located verbatim in the source · abstract, characters 1012–1117 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Suitable AgNO3 concentrations decreased H2O2 content and production rates during light treatment.

Treatment
apt concentration of AgNO3e1
Outcome
H2O2 content and production ratese0
Direction
decreasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“while H2O2 content and production rates would decrease.”

✓ located verbatim in the source · abstract, characters 1119–1174 · show in text ↑
e1

“apt concentration of AgNO3”

✓ located verbatim in the source · abstract, characters 1091–1117 · show in text ↑
e2

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Higher AgNO3 concentrations raised H2O2 content and production rates again.

Treatment
higher concentration of AgNO3e0
Outcome
H2O2 content and production ratese0
Direction
risinge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“higher concentration of AgNO3 would cause H2O2 content and production rates rising again.”

✓ located verbatim in the source · abstract, characters 1192–1281 · show in text ↑
e1

“pear cultivars (Pyrus brestchneideri Rehd.)”

✓ located verbatim in the source · abstract, characters 155–198 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors judged 0.1 mg/L AgNO3 best for increasing antioxidant enzyme activity in both pear cultivars.

Treatment
0.1 mg/L AgNO3e0
Outcome
increase the activity of antioxidant enzymese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“0.1 mg/L AgNO3 was the best to increase the activity of antioxidant enzymes in the leaves of both Jjinshui and Jinhua pear cultivars among the 4 treatments.”

✓ located verbatim in the source · abstract, characters 1392–1548 · show in text ↑