Abstract
Pine wilt disease caused by pine wood nematodes,Bursaphelenchus xylophilus,isone of main forest disease.It was reported that pathogenic microbes accompanying pine wood nematodes and microbial endophytes of pine tree were involved in this disease.A primarily study on inhibitive effects of strain C,isolated from cultured pine wood nematodes,on pine wilt disease was carried out in the laboratory.Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively.The results showed that both relative transpiration intensity and relative weight of twigs inoculated with nematodes were the least,then those of twigs inoculated with the mixture and those of twigs inoculated with strain C.Fifteen days after treatment,relative weight of twigs inoculated with nematodes was significantly less than that of twigs inoculated with strain C(p0.05).Survival days of twigs inoculated with nematodes was significantly less than that of the others(p0.05).Number of twigs with brown stain leaves of pine twigs inoculated with nematodes was significantly more than that of twigs inoculated with strain C(p0.05).There was no significant difference between numbers of nematodes separated from twigs inoculated with nematodes and twigs inoculated with the mixture.8-monthold seedlings of P.massoniana without roots were cultural exercised in filtrate of Czaper's medium of strain C and both their infected rate and disease index were significantly less than those in control treatment in 6 days later(p0.05).These showed that strain C had inhibitive effects on this disease and some metabolites from strain C was in favor of antidisease and survival of pine seedlings.It was suggested that strain C inhibited pathogenic microbes accompanying nematodes and microbial endophytes of pine tree,or improved vitality and anti-adversity ability of pine tree.Strain C was identified as a strain of microzyme,Candida guiliermondii,by being observed under electron microscope and referring to API 20 C AUX identification system.
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Claims 7
Claim · model-written summary
Relative transpiration intensity and relative weight of Pinus massoniana twigs were lowest when inoculated with pine wood nematodes.
- Taxon
- Pinus massoniana
Pinus massonianae0 - Treatment
- inoculated with pine wood nematodese0
- Interactor
- pine wood nematodes
Bursaphelenchuse0pathogen - Outcome
- relative transpiration intensity and relative weighte1
- Direction
- the leaste1
- Comparator
- twigs inoculated with the mixturee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively”
e1“The results showed that both relative transpiration intensity and relative weight of twigs inoculated with nematodes were the least,then those of twigs inoculated with the mixture and those of twigs inoculated with strain C”
Claim · model-written summary
Fifteen days after inoculation, relative weight of Pinus massoniana twigs with pine wood nematodes was significantly lower than with strain C.
- Taxon
- Pinus massonianaPinus massonianae0
- Treatment
- inoculated with pine wood nematodese0
- Interactor
- pine wood nematodesBursaphelenchuse0pathogen
- Outcome
- relative weight of twigse2
- Direction
- lesse2
- Qualifier
- Fifteen days after treatmente2
- Comparator
- twigs inoculated with strain Ce2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively”
e2“Fifteen days after treatment, relative weight of twigs inoculated with nematodes was significantly less than that of twigs inoculated with strain C”
Claim · model-written summary
Survival days of Pinus massoniana twigs inoculated with pine wood nematodes were significantly fewer than in the other treatments.
- Taxon
- Pinus massonianaPinus massonianae0
- Treatment
- inoculated with pine wood nematodese0
- Interactor
- pine wood nematodesBursaphelenchuse0pathogen
- Outcome
- Survival days of twigse3
- Direction
- lesse3
- Comparator
- the otherse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively”
e3“Survival days of twigs inoculated with nematodes was significantly less than that of the others”
Claim · model-written summary
Twigs with brown-stained leaves were significantly more numerous with pine wood nematodes than with strain C.
- Taxon
- Pinus massonianaPinus massonianae0
- Treatment
- inoculated with pine wood nematodese0
- Interactor
- pine wood nematodesBursaphelenchuse0pathogen
- Outcome
- Number of twigs with brown stain leavese4
- Direction
- moree4
- Comparator
- twigs inoculated with strain Ce4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively”
e4“Number of twigs with brown stain leaves of pine twigs inoculated with nematodes was significantly more than that of twigs inoculated with strain C”
Claim · model-written summary
Numbers of pine wood nematodes recovered from nematode-inoculated and mixture-inoculated twigs did not differ significantly.
- Taxon
- Pinus massonianaPinus massonianae0
- Treatment
- inoculated with pine wood nematodese0
- Interactor
- pine wood nematodesBursaphelenchuse0pathogen
- Outcome
- numbers of nematodes separated from twigse5
- Direction
- no significant differencee5
- Comparator
- twigs inoculated with the mixturee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively”
e5“There was no significant difference between numbers of nematodes separated from twigs inoculated with nematodes and twigs inoculated with the mixture”
Claim · model-written summary
Filtrate of strain C medium reduced infection rate and disease index of Pinus massoniana seedlings compared with control.
- Taxon
- Pinus massonianaPinus massonianae0
- Treatment
- filtrate of Czaper's medium of strain Ce6
- Outcome
- infected rate and disease indexe7
- Direction
- lesse7
- Comparator
- control treatmente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively”
e6“8-monthold seedlings of P.massoniana without roots were cultural exercised in filtrate of Czaper's medium of strain C”
e7“both their infected rate and disease index were significantly less than those in control treatment in 6 days later”
Claim · model-written summary
Strain C was identified as Candida guilliermondii by electron microscopy and the API 20 C AUX system.
- Taxon
- Pinus massonianaPinus massonianae0
- Material
- strain Ce7
- Interactor
- Candida guiliermondiie7other
- Outcome
- identifiede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aquacultural exercised twigs of Pinus massoniana were inoculated with pine wood nematodes,strain C,and the mixture of the two former,respectively”
e7“Strain C was identified as a strain of microzyme,Candida guiliermondii,by being observed under electron microscope and referring to API 20 C AUX identification system”
The same questions in other papers
Bursaphelenchus · survival · Pinus massoniana (Chinese red pine)Positive and negative results
1 in the opposite direction
- positive
inoculation · survival · Pinus massoniana (Chinese red pine)Positive and negative results
2 in the opposite direction
- positive
- positive
inoculation · physiology: gas exchange · Pinus massoniana (Chinese red pine)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.