Abstract
【Objective】 The objective of this study is to research the impact of glyphosate on growth and fecundity of glyphosate-tolerant soybean and to provide statistics and a theoretical basis of time and concentration for weeding control by glyphosate.【Method】A random complete block design was used and the influence of spraying 41% glyphosate isopropylammonium AS at different rates on growth and yield component of transgenic soybean cultivar GTS-40-3-2 at V1-V5 stages was studied in the field.Also,the effect of glyphosate on weeding control was investigated.【Result】 It was found that glyphosate was effective at 1.23-12.30 kg·ai·hm-2 to control weed.However,the glyphosate more than recommended rate of 1.23-2.46 kg·ai·hm-2 depressed the number of stem nods and compound leaves,and reduced the number of seeds and seeds weight per plant of GTS-40-3-2.There were no obviously negative influence of spraying glyphosate on number of effective pods per plant and 100-seed-weight of GTS-40-3-2,and the main agronomic traits related to yield including plant height,pod height and number of effective embranchments even increased after dealing with glyphosate at lower concentration.There were different effects of spraying glyphosate on growth and fecundity of GTS-40-3-2 at different growth stages.Glyphosate of 4.92-7.38 kg·ai·hm-2 restrained distinctly soybean growth and yield on V1 and V2 stages,but did not injure yield component at V3-V5 stages.The stem nodes and leaves of GTS-40-3-2 were decreased in 10-20 days after treatment(DAT) with glyphosate at V3-V5 stages,then returned to control in 30 d.Glyphosate at 9.84-12.30 kg·ai·hm-2 reduced the number of seeds and seed weight per plant of GTS-40-3-2 at V4 and V5 stages,but did not affect them at V3 stage.【Conclusion】 Spraying glyphosate at 1.23-2.46 kg·ai·hm-2 not only was available for weeding control but also maintained the growth and yield component of GTS-40-3-2,which could be safe to use at V1-V5 growth stages of the glyphosate-tolerant soybean.Application of gyphosate beyond 1.23-2.46 kg·ai·hm-2 depressed the seed number but did not affect the seed mass of GTS-40-3-2.There were differences in growth and yield component depression by glyphosate between growth stages of GTS-40-3-2.Soybean at V1 and V2 stages was more sensitive to glyphosate than V3-V5.It was most safe to spray glyphosate at V3 stage compared with other stages in consideration of soybean growth and fecundity.
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Claims 14
Claim · model-written summary
Glyphosate at 1.23-12.30 kg active ingredient per hectare controlled weeds.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- glyphosatee0
- Interactor
- weede0weed
- Outcome
- control weede0
- Qualifier
- 1.23-12.30 kg·ai·hm-2e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“glyphosate was effective at 1.23-12.30 kg·ai·hm-2 to control weed”
t0“Glyphosate-Tolerant Soybean”
Claim · model-written summary
Above-recommended glyphosate rates depressed stem node and compound leaf counts.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- glyphosate more than recommended rate of 1.23-2.46 kg·ai·hm-2e0
- Outcome
- number of stem nods and compound leavese0
- Direction
- depressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“glyphosate more than recommended rate of 1.23-2.46 kg·ai·hm-2 depressed the number of stem nods and compound leaves”
t0“Soybean”
Claim · model-written summary
Above-recommended glyphosate rates reduced seeds per plant and seed weight per plant.
- Taxon
- Soybean
Glycine maxt0 - Material
- GTS-40-3-2e0
- Treatment
- glyphosate more than recommended rate of 1.23-2.46 kg·ai·hm-2e0
- Outcome
- number of seeds and seeds weight per plante0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“glyphosate more than recommended rate of 1.23-2.46 kg·ai·hm-2 depressed the number of stem nods and compound leaves,and reduced the number of seeds and seeds weight per plant of GTS-40-3-2”
t0“Soybean”
Claim · model-written summary
Glyphosate spraying had no obvious negative influence on effective pod number and 100-seed weight.
- Taxon
- Soybean
Glycine maxt0 - Material
- GTS-40-3-2e0
- Treatment
- spraying glyphosatee0
- Outcome
- number of effective pods per plant and 100-seed-weighte0
- Direction
- no obviously negative influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“no obviously negative influence of spraying glyphosate on number of effective pods per plant and 100-seed-weight of GTS-40-3-2”
t0“Soybean”
Claim · model-written summary
Lower glyphosate concentrations increased plant height, pod height and effective branch number.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- glyphosate at lower concentratione0
- Outcome
- plant height,pod height and number of effective embranchmentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“plant height,pod height and number of effective embranchments even increased after dealing with glyphosate at lower concentration”
t0“Soybean”
Claim · model-written summary
At V1 and V2 stages, 4.92-7.38 kg glyphosate restrained soybean growth.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- V1 and V2 stagese0
- Treatment
- Glyphosate of 4.92-7.38 kg·ai·hm-2e0
- Outcome
- soybean growthe0
- Direction
- restrainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Glyphosate of 4.92-7.38 kg·ai·hm-2 restrained distinctly soybean growth and yield on V1 and V2 stages”
Claim · model-written summary
At V1 and V2 stages, 4.92-7.38 kg glyphosate restrained soybean yield.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- V1 and V2 stagese0
- Treatment
- Glyphosate of 4.92-7.38 kg·ai·hm-2e0
- Outcome
- yielde0
- Direction
- restrainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Glyphosate of 4.92-7.38 kg·ai·hm-2 restrained distinctly soybean growth and yield on V1 and V2 stages”
Claim · model-written summary
At V3-V5 stages, 4.92-7.38 kg glyphosate did not injure yield components.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- V3-V5 stagese0
- Treatment
- Glyphosate of 4.92-7.38 kg·ai·hm-2e0
- Outcome
- yield componente0
- Direction
- did not injuree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Glyphosate of 4.92-7.38 kg·ai·hm-2 restrained distinctly soybean growth and yield on V1 and V2 stages,but did not injure yield component at V3-V5 stages”
Claim · model-written summary
At V3-V5 stages, glyphosate reduced stem nodes and leaves 10-20 days after treatment.
- Taxon
- Soybean
Glycine maxt0 - Material
- GTS-40-3-2e0
- Organ / stage
- V3-V5 stagese0
- Treatment
- glyphosatee0
- Outcome
- stem nodes and leavese0
- Direction
- decreasede0
- Qualifier
- 10-20 days after treatment(DAT)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“stem nodes and leaves of GTS-40-3-2 were decreased in 10-20 days after treatment(DAT) with glyphosate at V3-V5 stages”
t0“Soybean”
Claim · model-written summary
Stem nodes and leaves returned to control levels by day 30 after glyphosate at V3-V5.
- Taxon
- Soybean
Glycine maxt0 - Material
- GTS-40-3-2e0
- Organ / stage
- V3-V5 stagese0
- Treatment
- glyphosatee0
- Outcome
- stem nodes and leavese0
- Direction
- returned to controle0
- Qualifier
- 30 de0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“stem nodes and leaves of GTS-40-3-2 were decreased in 10-20 days after treatment(DAT) with glyphosate at V3-V5 stages,then returned to control in 30 d”
t0“Soybean”
Claim · model-written summary
At V4 and V5, 9.84-12.30 kg glyphosate reduced seeds per plant and seed weight.
- Taxon
- Soybean
Glycine maxt0 - Material
- GTS-40-3-2e0
- Organ / stage
- V4 and V5 stagese0
- Treatment
- Glyphosate at 9.84-12.30 kg·ai·hm-2e0
- Outcome
- number of seeds and seed weight per plante0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Glyphosate at 9.84-12.30 kg·ai·hm-2 reduced the number of seeds and seed weight per plant of GTS-40-3-2 at V4 and V5 stages”
t0“Soybean”
Claim · model-written summary
At V3, 9.84-12.30 kg glyphosate did not affect seeds per plant or seed weight.
- Taxon
- Soybean
Glycine maxt0 - Material
- GTS-40-3-2e0
- Organ / stage
- V3 stagee0
- Treatment
- Glyphosate at 9.84-12.30 kg·ai·hm-2e0
- Outcome
- number of seeds and seed weight per plante0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Glyphosate at 9.84-12.30 kg·ai·hm-2 reduced the number of seeds and seed weight per plant of GTS-40-3-2 at V4 and V5 stages,but did not affect them at V3 stage”
t0“Soybean”
Claim · model-written summary
The authors recommend 1.23-2.46 kg glyphosate at V1-V5 as safe for weed control and crop traits.
- Taxon
- soybean
Glycine maxe0 - Material
- GTS-40-3-2e0
- Treatment
- Spraying glyphosate at 1.23-2.46 kg·ai·hm-2e0
- Outcome
- safe to use at V1-V5 growth stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Spraying glyphosate at 1.23-2.46 kg·ai·hm-2 not only was available for weeding control but also maintained the growth and yield component of GTS-40-3-2,which could be safe to use at V1-V5 growth stages of the glyphosate-tolerant soybean”
Claim · model-written summary
The authors judged spraying at V3 the safest stage for soybean growth and fecundity.
- Taxon
- soybean
Glycine maxe0 - Outcome
- most safe to spray glyphosate at V3 stagee0
- Comparator
- other stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“It was most safe to spray glyphosate at V3 stage compared with other stages in consideration of soybean growth and fecundity”
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