Paper · 1999 · Chinese Journal of Appplied Environmental Biology

ESTABLISHMENT OF SWEET POTATO CELL SUSPENSION CULTURE AND ASSESSMENT OF ITS GROWTH

Kegui Chen

14
claims
30
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Cell suspension culture of two sweet potato( Ipomoea batatas L. Lam) genotypes, Nanshu 88 and 8129 4, was established from the calli derived from leaves. The growth curve of two clones of cell suspension was sigmoid, and the growth rate of Nanshu 88 was three times higher than that of 8129 4 on average. The cell fresh weight of 10 day culture of Nanshu 88 was 10 times higher than that of initiation. For Nanshu 88 optimal concentration of 2,4 dichlorophenoxyacetic acid (2,4 D) and sucrose in MS medium was 1.0 mg L -1 and 3 g L -1 respectively, and addition of coconut milk and yeast extract increased the growth. For 8129 4 the optimal concentration of 2,4 D and sucrose in the medium was 2.5 mg L -1 and 1.5 g L -1 respectively, and coconut milk had no effect on growth, but yeast extract stimulated growth dramatically. The casein hydrolysate had a little effect on the growth of the cell suspension culture, and single amino acids added into the medium (such as glutamine、tryptophan and proline) could not increase the growth at all. Glucose was shown to decrease the growth rate.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 14

Observationobservedoriginal resultmeasured

Claim · model-written summary

The growth curve of the two sweet potato cell suspension clones was sigmoid

Taxon
sweet potatoIpomoea batatase0
Outcome
growth curvee1
Qualifier
sigmoide1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e1

“The growth curve of two clones of cell suspension was sigmoid”

✓ located verbatim in the source · abstract, characters 154–215 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The growth rate of Nanshu 88 cell suspension was higher than that of 8129 4 on average

Taxon
sweet potatoIpomoea batatase0
Material
Nanshu 88e2
Outcome
growth ratee2
Direction
highere2
Value
three timese2
Qualifier
on averagee2
Comparator
8129 4e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e2

“the growth rate of Nanshu 88 was three times higher than that of 8129 4 on average”

✓ located verbatim in the source · abstract, characters 221–303 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The cell fresh weight of the 10 day culture of Nanshu 88 was 10 times higher than at initiation

Taxon
sweet potatoIpomoea batatase0
Material
Nanshu 88e3
Outcome
cell fresh weighte3
Direction
highere3
Value
10 timese3
Qualifier
10 day culturee3
Comparator
initiatione3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e3

“The cell fresh weight of 10 day culture of Nanshu 88 was 10 times higher than that of initiation”

✓ located verbatim in the source · abstract, characters 305–401 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

For Nanshu 88 the optimal 2,4-D concentration for cell suspension growth was 1.0 mg L-1

Taxon
sweet potatoIpomoea batatase0
Material
Nanshu 88e4
Treatment
2,4 dichlorophenoxyacetic acid (2,4 D)e4
Outcome
optimal concentratione4
Value
1.0 mg L -1e4

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e4

“For Nanshu 88 optimal concentration of 2,4 dichlorophenoxyacetic acid (2,4 D) and sucrose in MS medium was 1.0 mg L -1 and 3 g L -1 respectively, and addition of coconut milk and yeast extract increased the growth”

✓ located verbatim in the source · abstract, characters 403–616 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

For Nanshu 88 the optimal sucrose concentration for cell suspension growth was 3 g L-1

Taxon
sweet potatoIpomoea batatase0
Material
Nanshu 88e4
Treatment
sucrosee4
Outcome
optimal concentratione4
Value
3 g L -1e4

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e4

“For Nanshu 88 optimal concentration of 2,4 dichlorophenoxyacetic acid (2,4 D) and sucrose in MS medium was 1.0 mg L -1 and 3 g L -1 respectively, and addition of coconut milk and yeast extract increased the growth”

✓ located verbatim in the source · abstract, characters 403–616 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Addition of coconut milk increased the growth of Nanshu 88 cell suspension

Taxon
sweet potatoIpomoea batatase0
Material
Nanshu 88e4
Treatment
coconut milke4
Outcome
growthe4
Direction
increasede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e4

“For Nanshu 88 optimal concentration of 2,4 dichlorophenoxyacetic acid (2,4 D) and sucrose in MS medium was 1.0 mg L -1 and 3 g L -1 respectively, and addition of coconut milk and yeast extract increased the growth”

✓ located verbatim in the source · abstract, characters 403–616 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Addition of yeast extract increased the growth of Nanshu 88 cell suspension

Taxon
sweet potatoIpomoea batatase0
Material
Nanshu 88e4
Treatment
yeast extracte4
Outcome
growthe4
Direction
increasede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e4

“For Nanshu 88 optimal concentration of 2,4 dichlorophenoxyacetic acid (2,4 D) and sucrose in MS medium was 1.0 mg L -1 and 3 g L -1 respectively, and addition of coconut milk and yeast extract increased the growth”

✓ located verbatim in the source · abstract, characters 403–616 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

For 8129 4 the optimal 2,4-D concentration for cell suspension growth was 2.5 mg L-1

Taxon
sweet potatoIpomoea batatase0
Material
8129 4e5
Treatment
2,4 De5
Outcome
optimal concentratione5
Value
2.5 mg L -1e5

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e5

“For 8129 4 the optimal concentration of 2,4 D and sucrose in the medium was 2.5 mg L -1 and 1.5 g L -1 respectively”

✓ located verbatim in the source · abstract, characters 618–733 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

For 8129 4 the optimal sucrose concentration for cell suspension growth was 1.5 g L-1

Taxon
sweet potatoIpomoea batatase0
Material
8129 4e5
Treatment
sucrosee5
Outcome
optimal concentratione5
Value
1.5 g L -1e5

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e5

“For 8129 4 the optimal concentration of 2,4 D and sucrose in the medium was 2.5 mg L -1 and 1.5 g L -1 respectively”

✓ located verbatim in the source · abstract, characters 618–733 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Coconut milk had no effect on growth of 8129 4 cell suspension

Taxon
sweet potatoIpomoea batatase0
Material
8129 4e5
Treatment
coconut milke6
Outcome
growthe6
Direction
no effecte6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e5

“For 8129 4 the optimal concentration of 2,4 D and sucrose in the medium was 2.5 mg L -1 and 1.5 g L -1 respectively”

✓ located verbatim in the source · abstract, characters 618–733 · show in text ↑
e6

“coconut milk had no effect on growth, but yeast extract stimulated growth dramatically”

✓ located verbatim in the source · abstract, characters 739–825 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Yeast extract stimulated growth of 8129 4 cell suspension dramatically

Taxon
sweet potatoIpomoea batatase0
Material
8129 4e5
Treatment
yeast extracte6
Outcome
growthe6
Direction
stimulatede6
Qualifier
dramaticallye6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e5

“For 8129 4 the optimal concentration of 2,4 D and sucrose in the medium was 2.5 mg L -1 and 1.5 g L -1 respectively”

✓ located verbatim in the source · abstract, characters 618–733 · show in text ↑
e6

“coconut milk had no effect on growth, but yeast extract stimulated growth dramatically”

✓ located verbatim in the source · abstract, characters 739–825 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Casein hydrolysate had little effect on growth of the cell suspension culture

Taxon
sweet potatoIpomoea batatase0
Treatment
casein hydrolysatee7
Outcome
growthe7
Direction
no_changeinferrede7
Qualifier
a little effecte7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e7

“The casein hydrolysate had a little effect on the growth of the cell suspension culture”

✓ located verbatim in the source · abstract, characters 827–914 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Single amino acids added to the medium could not increase the growth of the cell suspension at all

Taxon
sweet potatoIpomoea batatase0
Treatment
single amino acidse7
Outcome
growthe7
Direction
could not increasee7
Qualifier
at alle7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e7

“single amino acids added into the medium (such as glutamine、tryptophan and proline) could not increase the growth at all”

✓ located verbatim in the source · abstract, characters 920–1040 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Glucose decreased the growth rate of the cell suspension culture

Taxon
sweet potatoIpomoea batatase0
Treatment
Glucosee7
Outcome
growth ratee7
Direction
decreasee7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“two sweet potato( Ipomoea batatas L. Lam) genotypes”

✓ located verbatim in the source · abstract, characters 27–78 · show in text ↑
e7

“Glucose was shown to decrease the growth rate”

✓ located verbatim in the source · abstract, characters 1042–1087 · show in text ↑

glucose · growth · Ipomoea batatas (sweet potato)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

sucrose · molecular: metabolites · Ipomoea batatas (sweet potato)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

botanical · growth · Ipomoea batatas (sweet potato)Positive and negative results

13 papers: 10 positive, 3 negative or no effect

9 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

9 papers more in this group

See all 13 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.