Abstract
Salicylic acid (SA) can induce stomatal closure with a concentration-dependent manner,the closed stomata can open again when the concentration of SA is between 1-1 000μmol·L~(-1),but when the concentration of SA is 0.01 mol·L~(-1),the change of closed stomata cannot happen.DPI 10μmol·L~(-1) weakened the effect of SA on stomatal apertures by 45%-60%,which indicated that the generation of H_2O_2 might be involved in the stomatal closure induced by SA.We directly examined the production of H_2O_2 by microinjection based on H_2DCFDA.SA 100μmol·L~(-1) by applied externally or microinjection could induce rapid increasing in fluores- cence of DCF in guard cells.In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts,but the production of H_2O_2 was inhibited in the regions of plasma membrane.These results suggest that the chloroplasts in guard cells might be the main source of H_2O_2 production,meanwhile,plasma membrane NADPH oxidase may also contribute to the H_2O_2 generation in the guard cells,when treated with SA.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Salicylic acid induced concentration-dependent stomatal closure.
- Taxon
- Vicia faba L.
Vicia fabat0 - Treatment
- Salicylic acid (SA)e0
- Outcome
- stomatal closuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salicylic acid (SA) can induce stomatal closure with a concentration-dependent manner”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
Closed stomata reopened at SA concentrations from 1 to 1 000 μmol·L~(-1).
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- SAe0
- Outcome
- closed stomata can open againe0
- Value
- 1-1 000μmol·L~(-1)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the closed stomata can open again when the concentration of SA is between 1-1 000μmol·L~(-1)”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
At 0.01 mol·L~(-1) SA, closed stomata did not reopen.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- SA is 0.01 mol·L~(-1)e0
- Outcome
- change of closed stomata cannot happene0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when the concentration of SA is 0.01 mol·L~(-1),the change of closed stomata cannot happen.”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
DPI weakened the SA effect on stomatal aperture by 45%-60%.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- DPI 10μmol·L~(-1)e0
- Outcome
- effect of SA on stomatal aperturese0
- Direction
- weakenede0
- Value
- 45%-60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DPI 10μmol·L~(-1) weakened the effect of SA on stomatal apertures by 45%-60%”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
Externally applied SA increased DCF fluorescence in guard cells.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- SA 100μmol·L~(-1) by applied externallye0
- Outcome
- fluores- cence of DCF in guard cellse0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA 100μmol·L~(-1) by applied externally or microinjection could induce rapid increasing in fluores- cence of DCF in guard cells.”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
Microinjected SA increased DCF fluorescence in guard cells.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- microinjectione0
- Outcome
- fluores- cence of DCF in guard cellse0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA 100μmol·L~(-1) by applied externally or microinjection could induce rapid increasing in fluores- cence of DCF in guard cells.”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
With DPI, SA increased DCF fluorescence only in chloroplast regions.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- DPIe0
- Outcome
- DCF fluorescence only in the regions of chloroplastse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
With DPI, SA-induced H_2O_2 production was inhibited at the plasma membrane.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- DPIe1
- Outcome
- production of H_2O_2e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the production of H_2O_2 was inhibited in the regions of plasma membrane.”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
The authors suggest chloroplasts are the main guard-cell H_2O_2 source.
- Taxon
- Vicia faba L.Vicia fabat0
- Organ / stage
- guard cellse0
- Outcome
- H_2O_2 productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chloroplasts in guard cells might be the main source of H_2O_2 production”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
Claim · model-written summary
The authors suggest plasma-membrane NADPH oxidase also contributes to guard-cell H_2O_2 generation under SA.
- Taxon
- Vicia faba L.Vicia fabat0
- Gene
- NADPH oxidasee0
- Outcome
- H_2O_2 generation in the guard cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plasma membrane NADPH oxidase may also contribute to the H_2O_2 generation in the guard cells,when treated with SA.”
t0“Involvement of NADPH Oxidase in Stomatal Closure Induced by Salicylic Acid in Vicia faba L.”
e1“In the presence of DPI,the guard cells treated with SA 100μmol·L~(-1) showed increase in DCF fluorescence only in the regions of chloroplasts”
The same questions in other papers
salicylic acid · nutrient: content · Vicia faba (faba bean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
salicylic acid · physiology: gas exchange · Vicia faba (faba bean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.