Abstract
Agronomic practices affect plant growth, development, and productivity through modifications of plant physiological processes. The effect of cover crop (hairy vetch [ Vicia villosa Roth subsp. villosa ]), no‐tillage, and N fertilizer side‐dressing on plant and soil health and expression of maize ( Zea mays L.) genes was measured throughout the growing season. Expression of ACS , GS2 , ICDH , NiR , and PEPC genes involved in C and N metabolism was determined. No‐tillage led to a greener and better hydrated maize crop at V6, greater soil moisture at V6 and silking, and greater plant height throughout the season. Cover crop resulted in higher leaf N at V6, greener crop throughout the season, and taller plants at silking and grain fill. There were signs of N deficiency in no‐tillage from silking onward, which was reflected in decreased greenness at silking and reduced soil nitrate at grain fill. Expression of GS2 , NiR , and PEPC was stimulated by tillage later in the season, possibly as a result of greater soil N availability or greater gene expression because fewer leaves remained on these plants. Grain yields were unaffected by tillage but increased by cover crop. It appeared that low‐moisture stress in no‐tillage led to greater plant vigor, but this did not translate into increased yield because of N stress. This study highlights the need to have a better understanding of global gene expression by soil management practices, which can help select and introgress specific genes for greater exploitation of the genetic potential of maize especially when grown under no‐tillage.
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Claims 11
Claim · model-written summary
No-tillage increased maize greenness and hydration at V6.
- Organ / stage
- V6e0
- Treatment
- No‐tillagee0
- Outcome
- greener and better hydrated maize crope0
- Direction
- greenere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“No‐tillage led to a greener and better hydrated maize crop at V6, greater soil moisture at V6 and silking, and greater plant height throughout the season.”
Claim · model-written summary
No-tillage increased soil moisture at V6 and silking.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“No‐tillage led to a greener and better hydrated maize crop at V6, greater soil moisture at V6 and silking, and greater plant height throughout the season.”
Claim · model-written summary
No-tillage increased plant height throughout the season.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“No‐tillage led to a greener and better hydrated maize crop at V6, greater soil moisture at V6 and silking, and greater plant height throughout the season.”
Claim · model-written summary
Cover crop increased maize leaf nitrogen at V6.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Cover crop resulted in higher leaf N at V6, greener crop throughout the season, and taller plants at silking and grain fill.”
t0“Maize”
Claim · model-written summary
Cover crop increased crop greenness throughout the season.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Cover crop resulted in higher leaf N at V6, greener crop throughout the season, and taller plants at silking and grain fill.”
t0“Maize”
Claim · model-written summary
Cover crop increased height at silking and grain fill.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Cover crop resulted in higher leaf N at V6, greener crop throughout the season, and taller plants at silking and grain fill.”
t0“Maize”
Claim · model-written summary
No-tillage reduced crop greenness at silking.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“There were signs of N deficiency in no‐tillage from silking onward, which was reflected in decreased greenness at silking and reduced soil nitrate at grain fill.”
t0“Maize”
Claim · model-written summary
No-tillage reduced soil nitrate at grain fill.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“There were signs of N deficiency in no‐tillage from silking onward, which was reflected in decreased greenness at silking and reduced soil nitrate at grain fill.”
t0“Maize”
Claim · model-written summary
Tillage stimulated later-season expression of GS2, NiR and PEPC.
- Treatment
- tillagee0
- Gene
- GS2 , NiR , and PEPCe0
- Outcome
- Expression of GS2 , NiR , and PEPCe0
- Direction
- stimulatede0
- Qualifier
- later in the seasone0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Expression of GS2 , NiR , and PEPC was stimulated by tillage later in the season, possibly as a result of greater soil N availability or greater gene expression because fewer leaves remained on these plants.”
t0“Maize”
Claim · model-written summary
Grain yields were unaffected by tillage.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Grain yields were unaffected by tillage but increased by cover crop.”
t0“Maize”
Claim · model-written summary
Cover crop increased grain yield.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Grain yields were unaffected by tillage but increased by cover crop.”
t0“Maize”
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