Abstract
This study investigated the tolerance to desiccation and freezing of tea seeds, embryonic axes (EAs) and cotyledonary embryonic axes (CEAs, consisting of EAs with portions of cotyledons still attached). No seeds germinated after desiccation and cryopreservation. EAs extracted from seeds desiccated to 18.9% moisture content (fresh weight basis) and cryopreserved showed 20.7% survival but plantlet production from these EAs was impossible. When EAs and CEAs were extracted from seeds before being submitted to desiccation and freezing, survival of control and frozen samples was equivalent with both types of materials. However, plantlet production was significantly higher from control and cryopreserved CEAs than EAs. The maturity stage of the seeds from which CEAs were extracted had an important effect on their survival and plant production percentages, mature seeds providing better results than early mature and late mature seeds. The highest percentages of plantlet production from cryopreserved CEAs, which ranged between 75.1 and 80.4%, were achieved for EA moisture contents between 21.5 and 15.0%.
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Claims 9
Claim · model-written summary
No tea seeds germinated after desiccation and cryopreservation.
Not reportedComparatorDirection
Verbatim
t0“tea (Camellia sinensis L.)”
e0“No seeds germinated after desiccation and cryopreservation.”
Claim · model-written summary
Embryonic axes from seeds desiccated to 18.9% moisture content and cryopreserved had 20.7% survival.
- Taxon
- teat0
- Organ / stage
- EAse0
- Treatment
- desiccated to 18.9% moisture content (fresh weight basis) and cryopreservede0
- Outcome
- survivale0
- Value
- 20.7%e0
Not reportedDirection
Verbatim
t0“tea (Camellia sinensis L.)”
e0“EAs extracted from seeds desiccated to 18.9% moisture content (fresh weight basis) and cryopreserved showed 20.7% survival”
Claim · model-written summary
Those cryopreserved embryonic axes could not produce plantlets.
Not reportedDirection
Verbatim
t0“tea (Camellia sinensis L.)”
e0“plantlet production from these EAs was impossible”
Claim · model-written summary
For axes extracted before treatment, frozen and control samples survived equivalently for both EAs and CEAs.
Verbatim
t0“tea (Camellia sinensis L.)”
e0“When EAs and CEAs were extracted from seeds before being submitted to desiccation and freezing, survival of control and frozen samples was equivalent with both types of materials.”
Claim · model-written summary
Control CEAs produced significantly more plantlets than control EAs.
Verbatim
t0“tea (Camellia sinensis L.)”
e0“plantlet production was significantly higher from control and cryopreserved CEAs than EAs”
Claim · model-written summary
Cryopreserved CEAs produced significantly more plantlets than cryopreserved EAs.
Verbatim
t0“tea (Camellia sinensis L.)”
e0“plantlet production was significantly higher from control and cryopreserved CEAs than EAs”
Claim · model-written summary
CEAs from mature seeds survived better than those from early or late mature seeds.
Verbatim
t0“tea (Camellia sinensis L.)”
e0“The maturity stage of the seeds from which CEAs were extracted had an important effect on their survival and plant production percentages, mature seeds providing better results than early mature and late mature seeds.”
Claim · model-written summary
CEAs from mature seeds produced more plants than those from early or late mature seeds.
Verbatim
t0“tea (Camellia sinensis L.)”
e0“The maturity stage of the seeds from which CEAs were extracted had an important effect on their survival and plant production percentages, mature seeds providing better results than early mature and late mature seeds.”
Claim · model-written summary
Cryopreserved CEAs reached 75.1 to 80.4% plantlet production at EA moisture contents of 21.5 to 15.0%.
Verbatim
t0“tea (Camellia sinensis L.)”
e0“The highest percentages of plantlet production from cryopreserved CEAs, which ranged between 75.1 and 80.4%, were achieved for EA moisture contents between 21.5 and 15.0%.”
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