Abstract
Tea, one of the world’s three major non-alcoholic beverages, sustains enormous annual consumption globally. However, the growth and development time of tea trees is long, the planting is affected by time and region, and it is not conducive to the diversification and innovation of tea varieties. To establish an efficient in vitro regeneration system for tea plant, the effects of culture conditions and plant growth regulators (PGRs) on adventitious bud differentiation and rooting were investigated in this study. The large-leaf tea variety ‘Yunkang 10’ was used for explant collection and the MS (Murashige and Skoog) medium was adopted as the basal medium. Callus induction peaked at 100% on the MS medium supplemented with 3.0 mg/L 6-benzylaminopurine (BAP) and 0.2 mg/L naphthaleneacetic acid (NAA). Optimal subculture occurred on MS + 3.0 mg/L BAP + 0.3 mg/L NAA + 3.0 mg/L gibberellic acid (GA 3 ), while maximal proliferation (> 300% increase) used MS + 0.2 mg/L BAP + 0.1 mg/L NAA + 3.0 mg/L GA 3 . Nine-month-old calli showed highest bud differentiation (24.73% per callus) on MS + 2.0 mg/L BAP + 0.2 mg/L NAA and adventitious bud proliferation reached 89.64% on MS + 1.5 mg/L BAP + 0.1 mg/L NAA. Paraffin sectioning of calli at different stages confirmed adventitious bud origin. For the rooting induction rate, it peaked at 78.56% on 1/8 MS + 3.0 mg/L indole-3-butyric acid (IBA). Then the tissue cultured seedlings were moved into the field, and the transplantation using soil: humus: perlite = 6:3:1 achieved 71.11% survival. Further Start Codon Targeted (SCoT) marker analysis confirmed the genetic fidelity in the regenerated tea plants. The optimized system developed here enhances tea propagation and supports future genetic engineering efforts.
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Claims 9
Claim · model-written summary
Callus induction peaked at 100% on MS with BAP and NAA.
- Taxon
- tea plant (Camellia sinensis)
Camellia sinensist0 - Material
- ‘Yunkang 10’t1
- Treatment
- MS medium supplemented with 3.0 mg/L 6-benzylaminopurine (BAP) and 0.2 mg/L naphthaleneacetic acid (NAA)e0
- Outcome
- Callus inductione0
- Direction
- peakede0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction peaked at 100% on the MS medium supplemented with 3.0 mg/L 6-benzylaminopurine (BAP) and 0.2 mg/L naphthaleneacetic acid (NAA).”
t0“tea plant (Camellia sinensis)”
t1“‘Yunkang 10’”
Claim · model-written summary
MS with BAP, NAA and GA 3 was optimal for subculture.
- Taxon
- tea plant (Camellia sinensis)Camellia sinensist0
- Treatment
- MS + 3.0 mg/L BAP + 0.3 mg/L NAA + 3.0 mg/L gibberellic acid (GA 3 )e0
- Outcome
- subculturee0
- Direction
- Optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal subculture occurred on MS + 3.0 mg/L BAP + 0.3 mg/L NAA + 3.0 mg/L gibberellic acid (GA 3 )”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
MS with BAP, NAA and GA 3 gave maximal callus proliferation above 300%.
- Taxon
- tea plant (Camellia sinensis)Camellia sinensist0
- Treatment
- MS + 0.2 mg/L BAP + 0.1 mg/L NAA + 3.0 mg/L GA 3e0
- Outcome
- proliferatione0
- Direction
- maximale0
- Value
- > 300% increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximal proliferation (> 300% increase) used MS + 0.2 mg/L BAP + 0.1 mg/L NAA + 3.0 mg/L GA 3”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
Nine-month calli had highest bud differentiation, 24.73% per callus, on MS with BAP and NAA.
- Taxon
- tea plant (Camellia sinensis)Camellia sinensist0
- Organ / stage
- Nine-month-old callie0
- Treatment
- MS + 2.0 mg/L BAP + 0.2 mg/L NAAe0
- Outcome
- bud differentiatione0
- Direction
- higheste0
- Value
- 24.73% per calluse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nine-month-old calli showed highest bud differentiation (24.73% per callus) on MS + 2.0 mg/L BAP + 0.2 mg/L NAA”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
Adventitious bud proliferation reached 89.64% on MS with BAP and NAA.
- Taxon
- tea plant (Camellia sinensis)Camellia sinensist0
- Treatment
- MS + 1.5 mg/L BAP + 0.1 mg/L NAAe0
- Outcome
- adventitious bud proliferatione0
- Value
- 89.64%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“adventitious bud proliferation reached 89.64% on MS + 1.5 mg/L BAP + 0.1 mg/L NAA.”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
Paraffin sections confirmed the origin of adventitious buds.
- Taxon
- tea plant (Camellia sinensis)Camellia sinensist0
- Outcome
- adventitious bud origine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Paraffin sectioning of calli at different stages confirmed adventitious bud origin.”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
Root induction peaked at 78.56% on diluted MS with IBA.
- Taxon
- tea plant (Camellia sinensis)Camellia sinensist0
- Treatment
- 1/8 MS + 3.0 mg/L indole-3-butyric acid (IBA)e0
- Outcome
- rooting induction ratee0
- Direction
- peakede0
- Value
- 78.56%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For the rooting induction rate, it peaked at 78.56% on 1/8 MS + 3.0 mg/L indole-3-butyric acid (IBA).”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
A soil, humus and perlite mixture yielded 71.11% transplant survival.
- Taxon
- tea plant (Camellia sinensis)Camellia sinensist0
- Treatment
- soil: humus: perlite = 6:3:1e0
- Outcome
- survivale0
- Value
- 71.11%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transplantation using soil: humus: perlite = 6:3:1 achieved 71.11% survival.”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
SCoT marker analysis confirmed genetic fidelity of regenerated plants.
Not reportedComparator
Verbatim
e0“Further Start Codon Targeted (SCoT) marker analysis confirmed the genetic fidelity in the regenerated tea plants.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.