Abstract
BACKGROUND: Most plant species produce seeds that can be dried to c. 3-7% moisture content, i.e., orthodox, and successfully stored in liquid nitrogen. However, tea seeds are often described as recalcitrant, i.e., they die when to lower moisture levels safe for cryopreservation. OBJECTIVE: In this study, we investigated the tolerance to desiccation and freezing of Longjing tea (Camellia sinensis) seeds and analyzed the reason behind seed mortality during desiccation and cryopreservation. MATERIALS AND METHODS: We analyzed the desiccation capacity of tea seeds from Hangzhou, China and subjected the desiccated seeds to programmed cooling and thermal analysis using differential scanning calorimeter (DSC). Subsequently, we used the exotherms to determine the frozen and unfrozen water. We analyzed seed germination and seedling establishment at different water content after storing seeds in liquid nitrogen and correlated the survival with the bound and unbound water. RESULTS: Results showed that the seeds could tolerate drying to 7.9 % moisture content without significant viability loss. Moreover, the germination percentage declined sharply after cryopreservation, and the lowest moisture content at which a normal seedling could develop was 11.3+/-1 %, although seeds dried to lower MC did germinate but did not grow into the seedling phase. CONCLUSION: Longjing tea seeds used in this study were neither orthodox nor recalcitrant. We show that the interaction between lipids and freezable water in seeds may lead to the cryoinjury. https://doi.org/10.54680/fr24610110612.
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Claims 6
Claim · model-written summary
Tea seeds tolerated drying to 7.9% moisture content without significant viability loss.
- Taxon
- teat0
- Treatment
- drying to 7.9 % moisture contente0
- Outcome
- viability losse0
- Direction
- without significant viability losse0
- Value
- 7.9 %e0
Not reportedComparator
Verbatim
e0“the seeds could tolerate drying to 7.9 % moisture content without significant viability loss”
t0“Longjing Tea Seeds”
Claim · model-written summary
Tea seed germination declined sharply after cryopreservation.
Not reportedComparator
Verbatim
e0“the germination percentage declined sharply after cryopreservation”
t0“Longjing Tea Seeds”
Claim · model-written summary
The lowest moisture content supporting normal tea seedling development was 11.3+/-1 %.
Verbatim
e0“the lowest moisture content at which a normal seedling could develop was 11.3+/-1 %”
t0“Longjing Tea Seeds”
Claim · model-written summary
Tea seeds dried to lower moisture content germinated but failed to reach the seedling phase.
Verbatim
e0“seeds dried to lower MC did germinate but did not grow into the seedling phase”
t0“Longjing Tea Seeds”
Claim · model-written summary
The studied Longjing tea seeds were neither orthodox nor recalcitrant.
Verbatim
e0“Longjing tea seeds used in this study were neither orthodox nor recalcitrant”
Claim · model-written summary
Interaction between lipids and freezable water may cause cryoinjury in tea seeds.
Verbatim
e0“the interaction between lipids and freezable water in seeds may lead to the cryoinjury”
t0“Longjing Tea Seeds”
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