Abstract
Introduction Adventitious root (AR) development, affected by various biotic and abiotic factors, is the most important procedure in tea plant ( Camellia sinensis L.) cutting propagation. Establishing symbiotic relationships with most terrestrial plants, AMF (Arbuscular mycorrhizal fungus) can mediate the AR formation of several herbaceous and woody plants in previous studies. Methods In this paper, effects of combined application of AMF and exogenous auxin on AR formation of cuttings from different tea plant varieties (‘Pingyangtezao’, ‘Longjing 43’ and ‘Longjingchangye’) were studied. Then we also performed RNA-Seq analysis with ‘Pingyangtezao’ cuttings aiming to find the possible auxin-related pathway of AM fungal regulation on AR formation. To accurately uncover the regulatory mechanism of AMF on AR formation of tea cuttings, rooting process were separated into four stages (S0, non-rooting; S1, AR protrusion; S2, AR formation and S3, AR elongation) at the same sampling time. Results and Discussion Results showed that IBA treatment increased the mycorrhizal colonization rate, especially in ‘Pingyangtezao’ variety (from 37.58% to 46.29%). Both inoculating AMF and addition of IBA promoted the AR formation, and rooting of different tea plant varieties showed different dependence on auxin. AMF could alleviate the effect of auxin-related inhibitors (2,3,5-triiodobenzoic acid, L-α-(Aminooxy)-β-phenylpropionic acid and α-(phenylethyl-2-oxo)-IAA) on rooting of tea cuttings, even though the colonization of AMF was hindered at various degrees. Transcriptomic analysis showed that different numbers of differentially expressed genes (DEGs) at various rooting stages of tea cuttings with the most at S2 stage (1360 DEGs), indicating the increasing regulation by AMF with the development of AR. Similar trend was found in auxin-related DEGs, and family genes of YUC , GH , PIN , LAX , SAUR , AUX , and ABP involved in the AM fungal regulation on AR formation of tea cuttings. Additionally, AMF strongly mediated auxin transport and signal transduction pathways in tea cuttings as showed by the results of correlation analysis. Overall, interaction of AMF and exogenous auxin in promoting rooting and the preliminary mechanism of AMF regulating AR formation of tea cuttings was deciphered in this paper, which may provide a basis for further deep mechanistic research and cutting propagation of tea production.
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Claims 12
Claim · model-written summary
IBA increased mycorrhizal colonization in Pingyangtezao tea from 37.58% to 46.29%.
- Taxon
- tea plantt0
- Material
- ‘Pingyangtezao’e0
- Treatment
- IBA treatmente0
- Outcome
- mycorrhizal colonization ratee0
- Direction
- increasede0
- Value
- 37.58% to 46.29%e0
Not reportedComparator
Verbatim
e0“IBA treatment increased the mycorrhizal colonization rate, especially in ‘Pingyangtezao’ variety (from 37.58% to 46.29%)”
t0“tea plant”
Claim · model-written summary
AMF inoculation promoted adventitious root formation in tea cuttings.
- Taxon
- tea plantt0
- Treatment
- inoculating AMFe0
- Interactor
- AMFe0symbiont
- Outcome
- AR formatione0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“Both inoculating AMF and addition of IBA promoted the AR formation”
t0“tea plant”
Claim · model-written summary
IBA addition promoted adventitious root formation in tea cuttings.
Not reportedComparator
Verbatim
e0“Both inoculating AMF and addition of IBA promoted the AR formation”
t0“tea plant”
Claim · model-written summary
Tea varieties differed in auxin dependence of rooting.
Verbatim
e0“rooting of different tea plant varieties showed different dependence on auxin”
Claim · model-written summary
AMF alleviated the rooting effect of three auxin-related inhibitors in tea cuttings.
Verbatim
e0“AMF could alleviate the effect of auxin-related inhibitors (2,3,5-triiodobenzoic acid, L-α-(Aminooxy)-β-phenylpropionic acid and α-(phenylethyl-2-oxo)-IAA) on rooting of tea cuttings”
Claim · model-written summary
Auxin-related inhibitors hindered AMF colonization to varying degrees.
Not reportedTreatmentComparator
Verbatim
e0“the colonization of AMF was hindered at various degrees”
t0“tea plant”
Claim · model-written summary
Tea cuttings had the most AMF-associated DEGs at S2 rooting stage, 1360.
Verbatim
e0“most at S2 stage (1360 DEGs)”
t0“tea plant”
Claim · model-written summary
Auxin-related DEGs showed a similar stage pattern during tea rooting.
Verbatim
e0“Similar trend was found in auxin-related DEGs”
t0“tea plant”
Claim · model-written summary
YUC, GH and PIN families were implicated in AMF regulation of tea adventitious roots.
- Taxon
- tea plantt0
- Interactor
- AM fungale0symbiont
- Gene
- YUC , GH , PINe0
- Outcome
- AR formation of tea cuttingse0
Not reportedDirection
Verbatim
e0“family genes of YUC , GH , PIN , LAX , SAUR , AUX , and ABP involved in the AM fungal regulation on AR formation of tea cuttings”
t0“tea plant”
Claim · model-written summary
LAX, SAUR and AUX families were implicated in AMF regulation of tea adventitious roots.
- Taxon
- tea plantt0
- Interactor
- AM fungale0symbiont
- Gene
- LAX , SAUR , AUXe0
- Outcome
- AR formation of tea cuttingse0
Not reportedDirection
Verbatim
e0“family genes of YUC , GH , PIN , LAX , SAUR , AUX , and ABP involved in the AM fungal regulation on AR formation of tea cuttings”
t0“tea plant”
Claim · model-written summary
ABP family genes were implicated in AMF regulation of tea adventitious roots.
Not reportedDirection
Verbatim
e0“ABP involved in the AM fungal regulation on AR formation of tea cuttings”
t0“tea plant”
Claim · model-written summary
Correlation analysis implicated AMF in tea auxin transport and signal transduction pathways.
Not reportedDirection
Verbatim
e0“AMF strongly mediated auxin transport and signal transduction pathways in tea cuttings as showed by the results of correlation analysis”
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