Abstract
AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Sequence analysis showed that the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain. It belongs to the group A subfamily of bZIP transcription factors. TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots. TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress, and its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis. TaAREB3 protein has transcriptional activation activity, and can bind to the ABRE cis-element in vitro. Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances. TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis. These results demonstrated that TaAREB3 plays an important role in drought and freezing tolerances in Arabidopsis.
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Claims 14
Claim · model-written summary
TaAREB3 was isolated from wheat.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaAREB3e0
- Outcome
- isolated from wheate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat.”
Claim · model-written summary
TaAREB3 has conserved terminal regions, a variable middle region and a bZIP domain.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaAREB3e0
- Outcome
- composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain”
t0“Wheat”
Claim · model-written summary
TaAREB3 belongs to group A of bZIP transcription factors.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- group A subfamily of bZIP transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It belongs to the group A subfamily of bZIP transcription factors.”
t0“Wheat”
Claim · model-written summary
TaAREB3 was expressed across listed organs, most highly in roots.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- rootse0
- Gene
- TaAREB3e0
- Outcome
- constitutively expressede0
- Direction
- most highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots.”
t0“Wheat”
Claim · model-written summary
ABA induced TaAREB3 expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- abscisic acid (ABA)e0
- Gene
- TaAREB3e0
- Outcome
- TaAREB3 gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress”
t0“Wheat”
Claim · model-written summary
Low temperature stress induced TaAREB3 expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- low temperature stresse0
- Gene
- TaAREB3e0
- Outcome
- TaAREB3 gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress”
t0“Wheat”
Claim · model-written summary
TaAREB3 protein localized to nuclei in tobacco epidermal cells and transgenic Arabidopsis.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- epidermal cellse0
- Gene
- TaAREB3t0
- Outcome
- localized in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis.”
t0“TaAREB3”
Claim · model-written summary
TaAREB3 has transcriptional activation activity.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaAREB3e0
- Outcome
- transcriptional activation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 protein has transcriptional activation activity”
t0“Wheat”
Claim · model-written summary
TaAREB3 binds the ABRE cis-element in vitro.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaAREB3t0
- Outcome
- bind to the ABRE cis-elemente0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can bind to the ABRE cis-element in vitro.”
t0“Wheat Transcription Factor TaAREB3”
Claim · model-written summary
TaAREB3 overexpression enhanced ABA sensitivity in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Overexpression of TaAREB3e0
- Gene
- TaAREB3e0
- Outcome
- ABA sensitivitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity”
Claim · model-written summary
TaAREB3 overexpression strengthened drought tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of TaAREB3e0
- Gene
- TaAREB3e0
- Outcome
- droughte0
- Direction
- strengthenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances.”
Claim · model-written summary
TaAREB3 overexpression strengthened freezing tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of TaAREB3e0
- Gene
- TaAREB3e0
- Outcome
- freezing tolerancese0
- Direction
- strengthenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances.”
Claim · model-written summary
TaAREB3 activated RD29A, RD29B and COR15A by binding their promoters in transgenic Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- TaAREB3e0
- Outcome
- activated RD29A, RD29B, COR15Ae0
- Qualifier
- binding to their promoter regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis.”
Claim · model-written summary
TaAREB3 activated COR47 by binding its promoter in transgenic Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- TaAREB3e0
- Outcome
- COR47e0
- Qualifier
- binding to their promoter regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis.”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Triticum aestivum (wheat)Positive and negative results
33 papers in the same direction as this paper · 9 in the opposite direction
- positive
- negative
- negative
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