Abstract
The study was conducted to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where the germination rate was 78.4%. The leaves were used as explants. Different concentration and combination of plant growth regulators (PGRs) were added with MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Leaf explants cultured on MS medium fortified with 3 mg/L BAP gave the highest number of shoots (3.5) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (5.149 cm) of plantlets, number (5.5) of leaves and fresh weight (0.781 g) of plantlets with the leaf explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (25.25) of roots/plantlet, length (8.785 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the leaf explants.
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Claims 8
Claim · model-written summary
Tomato seeds germinated at a rate of 78.4% on MS medium.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- MS mediume0
- Outcome
- germination ratee0
- Value
- 78.4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seeds were inoculated on MS medium where the germination rate was 78.4%.”
t0“tomato (Solanum lycopersicon Mill.)”
Claim · model-written summary
MS medium with 3 mg/L BAP produced the most shoots, 3.5 at 45 DAC.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- Leaf explantse0
- Treatment
- MS medium fortified with 3 mg/L BAPe0
- Outcome
- number of shootse0
- Direction
- higheste0
- Value
- 3.5e0
- Qualifier
- 45 DACe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf explants cultured on MS medium fortified with 3 mg/L BAP gave the highest number of shoots (3.5) at 45 DAC.”
t0“tomato (Solanum lycopersicon Mill.)”
Claim · model-written summary
At 45 DAC, 0.25 mg/L IAA produced the greatest plantlet length of 5.149 cm.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- plantletse0
- Treatment
- 0.25 mg/L IAAe0
- Outcome
- lengthe0
- Direction
- higheste0
- Value
- 5.149 cme0
- Qualifier
- 45 DACe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.25 mg/L IAA produced the highest length (5.149 cm) of plantlets, number (5.5) of leaves and fresh weight (0.781 g) of plantlets with the leaf explants at 45 DAC.”
t0“tomato (Solanum lycopersicon Mill.)”
Claim · model-written summary
At 45 DAC, 0.25 mg/L IAA produced the greatest leaf number of 5.5.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- leavese0
- Treatment
- 0.25 mg/L IAAe0
- Outcome
- numbere0
- Direction
- higheste0
- Value
- 5.5e0
- Qualifier
- 45 DACe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.25 mg/L IAA produced the highest length (5.149 cm) of plantlets, number (5.5) of leaves and fresh weight (0.781 g) of plantlets with the leaf explants at 45 DAC.”
t0“tomato (Solanum lycopersicon Mill.)”
Claim · model-written summary
At 45 DAC, 0.25 mg/L IAA produced the greatest plantlet fresh weight of 0.781 g.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- plantletse0
- Treatment
- 0.25 mg/L IAAe0
- Outcome
- fresh weighte0
- Direction
- higheste0
- Value
- 0.781 ge0
- Qualifier
- 45 DACe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.25 mg/L IAA produced the highest length (5.149 cm) of plantlets, number (5.5) of leaves and fresh weight (0.781 g) of plantlets with the leaf explants at 45 DAC.”
t0“tomato (Solanum lycopersicon Mill.)”
Claim · model-written summary
At 45 DAC, 0.5 mg/L IAA gave the most roots per plantlet, 25.25.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- roots/plantlete0
- Treatment
- 0.5 mg/L IAAe0
- Outcome
- numbere0
- Direction
- higheste0
- Value
- 25.25e0
- Qualifier
- 45 DACe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.5 mg/L IAA produced the highest number (25.25) of roots/plantlet, length (8.785 cm) of roots at 45 DAC”
t0“tomato (Solanum lycopersicon Mill.)”
Claim · model-written summary
At 45 DAC, 0.5 mg/L IAA gave the greatest root length, 8.785 cm.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- rootse0
- Treatment
- 0.5 mg/L IAAe0
- Outcome
- lengthe0
- Direction
- higheste0
- Value
- 8.785 cme0
- Qualifier
- 45 DACe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.5 mg/L IAA produced the highest number (25.25) of roots/plantlet, length (8.785 cm) of roots at 45 DAC”
t0“tomato (Solanum lycopersicon Mill.)”
Claim · model-written summary
The highest pot survival of regenerated tomato plantlets was 70.00%.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- in vitro regenerated plantletse0
- Outcome
- survival ratee0
- Direction
- higheste0
- Value
- 70.00 %e0
- Setting
- in the pote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the leaf explants.”
t0“tomato (Solanum lycopersicon Mill.)”
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