Abstract
Injection of Red Mexican bean leaves with Pseudomonas phascolicola Race 2 (compatible, 18 h before P. mors-prunorum or P. phaseolicola Race 1 (incompatible), or simultaneous inoculation with compatible and incompatible bacteria (3:1) greatly delayed the appearance of hypersensitive responses. When compatible bacteria were inoculated 12 h or less before incompatible bacteria, or when the ratio of these bacteria was 1:1 or 0.3:1 in simultaneous inoculation, hypersensitive responses did develop. Earlier inoculation with incompatible bacteria delayed the appearance and severity of disease symptoms following later inoculation with compatible bacteria. When selected areas of leaves were inoculated with compatible bacteria, effects on hypersensitive responses were confined to these areas when the whole leaf was inoculated later with incompatible bacteria. Inoculation through the upper leaf surface with incompatible bacteria did not affect susceptible responses when compatible bacteria were inoculated 24 h later through the lower surface. Treatment of leaves with heat-killed bacteria or live bacteria in numbers insufficient to cause hypersensitive responses did not prevent development of these responses following later inoculation with incompatible bacteria. Use of heat-killed bacteria did suppress hypersensitive responses in tobacco leaves. Injection of leaves with cycloheximide (20 p.p.m.) 30 min before inoculation with incompatible bacteria suppressed leakage of electrolytes and browning of tissues associated with hypersensitive responses. Cycloheximide had little effect on leakage of electrolytes from leaves inoculated with compatible bacteria or with the development of susceptible responses. Exposure of leaves to chloroform vapour delayed hypersensitive responses by 24 h; treatment with peroxidase had no effect.
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Claims 11
Claim · model-written summary
Pre-inoculation with compatible bacteria, or simultaneous inoculation with compatible and incompatible bacteria at 3:1, greatly delayed hypersensitive responses.
- Taxon
- Beant0
- Treatment
- simultaneous inoculation with compatible and incompatible bacteria (3:1)e1
- Interactor
- Pseudomonas phascolicola Race 2e1pathogen
- Second interactor
- P. phaseolicola Race 1e1pathogen
- Outcome
- appearance of hypersensitive responsese1
- Direction
- greatly delayede1
Not reportedComparator
Verbatim
t0“Bean Leaves”
e1“Injection of Red Mexican bean leaves with Pseudomonas phascolicola Race 2 (compatible, 18 h before P. mors-prunorum or P. phaseolicola Race 1 (incompatible), or simultaneous inoculation with compatible and incompatible bacteria (3:1) greatly delayed the appearance of hypersensitive responses”
Claim · model-written summary
Hypersensitive responses developed when compatible bacteria were inoculated 12 h or less before incompatible bacteria, or at 1:1 or 0.3:1 ratios.
- Taxon
- Beant0
- Treatment
- compatible bacteria were inoculated 12 h or less before incompatible bacteriae2
- Interactor
- compatible bacteriae2pathogen
- Outcome
- hypersensitive responsese2
Not reportedComparatorDirection
Verbatim
t0“Bean Leaves”
e2“When compatible bacteria were inoculated 12 h or less before incompatible bacteria, or when the ratio of these bacteria was 1:1 or 0.3:1 in simultaneous inoculation, hypersensitive responses did develop”
Claim · model-written summary
Earlier inoculation with incompatible bacteria delayed the appearance and severity of disease symptoms after later compatible inoculation.
- Taxon
- Beant0
- Treatment
- Earlier inoculation with incompatible bacteriae3
- Interactor
- compatible bacteriae3pathogen
- Outcome
- appearance and severity of disease symptomse3
- Direction
- delayede3
Not reportedComparator
Verbatim
t0“Bean Leaves”
e3“Earlier inoculation with incompatible bacteria delayed the appearance and severity of disease symptoms following later inoculation with compatible bacteria”
Claim · model-written summary
Compatible bacteria inoculated into selected leaf areas confined the effects on hypersensitive responses to those areas.
- Taxon
- Beant0
- Treatment
- selected areas of leaves were inoculated with compatible bacteriae4
- Interactor
- compatible bacteriae4pathogen
- Outcome
- effects on hypersensitive responsese4
- Qualifier
- confined to these arease4
Not reportedComparatorDirection
Verbatim
t0“Bean Leaves”
e4“When selected areas of leaves were inoculated with compatible bacteria, effects on hypersensitive responses were confined to these areas”
Claim · model-written summary
Incompatible bacteria inoculated through the upper leaf surface did not affect susceptible responses to compatible bacteria inoculated 24 h later.
- Taxon
- Beant0
- Treatment
- Inoculation through the upper leaf surface with incompatible bacteriae5
- Outcome
- susceptible responsese5
- Direction
- did not affecte5
Not reportedComparator
Verbatim
t0“Bean Leaves”
e5“Inoculation through the upper leaf surface with incompatible bacteria did not affect susceptible responses when compatible bacteria were inoculated 24 h later through the lower surface”
Claim · model-written summary
Heat-killed bacteria, or live bacteria in insufficient numbers, did not prevent development of hypersensitive responses to later incompatible bacteria.
- Taxon
- Beant0
- Treatment
- heat-killed bacteriae6
- Outcome
- development of these responsese6
- Direction
- did not prevente6
Not reportedComparator
Verbatim
t0“Bean Leaves”
e6“Treatment of leaves with heat-killed bacteria or live bacteria in numbers insufficient to cause hypersensitive responses did not prevent development of these responses”
Claim · model-written summary
Heat-killed bacteria suppressed hypersensitive responses in tobacco leaves.
- Taxon
- tobacco
Nicotiana tabacume7
- Treatment
- Use of heat-killed bacteriae7
- Outcome
- hypersensitive responsese7
- Direction
- suppresse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Use of heat-killed bacteria did suppress hypersensitive responses in tobacco leaves”
Claim · model-written summary
Cycloheximide (20 p.p.m.) injected 30 min before incompatible bacteria suppressed electrolyte leakage and browning associated with hypersensitive responses.
- Taxon
- Beant0
- Treatment
- cycloheximide (20 p.p.m.)e1
- Interactor
- incompatible bacteriae1pathogen
- Outcome
- leakage of electrolytes and browning of tissuese1
- Direction
- suppressede1
- Value
- 20 p.p.m.e1
Not reportedComparator
Verbatim
t0“Bean Leaves”
e1“Injection of leaves with cycloheximide (20 p.p.m.) 30 min before inoculation with incompatible bacteria suppressed leakage of electrolytes and browning of tissues associated with hypersensitive responses”
Claim · model-written summary
Cycloheximide had little effect on electrolyte leakage from leaves inoculated with compatible bacteria or on susceptible responses.
- Taxon
- Beant0
- Treatment
- Cycloheximidee1
- Outcome
- leakage of electrolytes from leaves inoculated with compatible bacteriae1
- Direction
- little effecte1
Not reportedComparator
Verbatim
t0“Bean Leaves”
e1“Cycloheximide had little effect on leakage of electrolytes from leaves inoculated with compatible bacteria or with the development of susceptible responses”
Claim · model-written summary
Chloroform vapour exposure delayed hypersensitive responses by 24 h.
- Taxon
- Beant0
- Treatment
- Exposure of leaves to chloroform vapoure1
- Outcome
- hypersensitive responsese1
- Direction
- delayede1
- Value
- 24 he1
Not reportedComparator
Verbatim
t0“Bean Leaves”
e1“Exposure of leaves to chloroform vapour delayed hypersensitive responses by 24 h”
Claim · model-written summary
Peroxidase treatment had no effect.
Not reportedComparator
Verbatim
t0“Bean Leaves”
e1“treatment with peroxidase had no effect”
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