Paper · 1977 · Annals of Botany

Alteration of Response of Bean Leaves to Compatible and Incompatible Bacteria

FRANCES LYON; R. K. S. WOOD

11
claims
21
verbatim quotes
12
citations
1
research areas
—
Not open access

Abstract

Injection of Red Mexican bean leaves with Pseudomonas phascolicola Race 2 (compatible, 18 h before P. mors-prunorum or P. phaseolicola Race 1 (incompatible), or simultaneous inoculation with compatible and incompatible bacteria (3:1) greatly delayed the appearance of hypersensitive responses. When compatible bacteria were inoculated 12 h or less before incompatible bacteria, or when the ratio of these bacteria was 1:1 or 0.3:1 in simultaneous inoculation, hypersensitive responses did develop. Earlier inoculation with incompatible bacteria delayed the appearance and severity of disease symptoms following later inoculation with compatible bacteria. When selected areas of leaves were inoculated with compatible bacteria, effects on hypersensitive responses were confined to these areas when the whole leaf was inoculated later with incompatible bacteria. Inoculation through the upper leaf surface with incompatible bacteria did not affect susceptible responses when compatible bacteria were inoculated 24 h later through the lower surface. Treatment of leaves with heat-killed bacteria or live bacteria in numbers insufficient to cause hypersensitive responses did not prevent development of these responses following later inoculation with incompatible bacteria. Use of heat-killed bacteria did suppress hypersensitive responses in tobacco leaves. Injection of leaves with cycloheximide (20 p.p.m.) 30 min before inoculation with incompatible bacteria suppressed leakage of electrolytes and browning of tissues associated with hypersensitive responses. Cycloheximide had little effect on leakage of electrolytes from leaves inoculated with compatible bacteria or with the development of susceptible responses. Exposure of leaves to chloroform vapour delayed hypersensitive responses by 24 h; treatment with peroxidase had no effect.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 11

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Pre-inoculation with compatible bacteria, or simultaneous inoculation with compatible and incompatible bacteria at 3:1, greatly delayed hypersensitive responses.

Taxon
Beant0
Treatment
simultaneous inoculation with compatible and incompatible bacteria (3:1)e1
Interactor
Pseudomonas phascolicola Race 2e1pathogen
Second interactor
P. phaseolicola Race 1e1pathogen
Outcome
appearance of hypersensitive responsese1
Direction
greatly delayede1

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e1

“Injection of Red Mexican bean leaves with Pseudomonas phascolicola Race 2 (compatible, 18 h before P. mors-prunorum or P. phaseolicola Race 1 (incompatible), or simultaneous inoculation with compatible and incompatible bacteria (3:1) greatly delayed the appearance of hypersensitive responses”

✓ located verbatim in the source · abstract, characters 0–292 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Hypersensitive responses developed when compatible bacteria were inoculated 12 h or less before incompatible bacteria, or at 1:1 or 0.3:1 ratios.

Taxon
Beant0
Treatment
compatible bacteria were inoculated 12 h or less before incompatible bacteriae2
Interactor
compatible bacteriae2pathogen
Outcome
hypersensitive responsese2

Not reportedComparatorDirection

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e2

“When compatible bacteria were inoculated 12 h or less before incompatible bacteria, or when the ratio of these bacteria was 1:1 or 0.3:1 in simultaneous inoculation, hypersensitive responses did develop”

✓ located verbatim in the source · abstract, characters 294–496 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Earlier inoculation with incompatible bacteria delayed the appearance and severity of disease symptoms after later compatible inoculation.

Taxon
Beant0
Treatment
Earlier inoculation with incompatible bacteriae3
Interactor
compatible bacteriae3pathogen
Outcome
appearance and severity of disease symptomse3
Direction
delayede3

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e3

“Earlier inoculation with incompatible bacteria delayed the appearance and severity of disease symptoms following later inoculation with compatible bacteria”

✓ located verbatim in the source · abstract, characters 498–653 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Compatible bacteria inoculated into selected leaf areas confined the effects on hypersensitive responses to those areas.

Taxon
Beant0
Treatment
selected areas of leaves were inoculated with compatible bacteriae4
Interactor
compatible bacteriae4pathogen
Outcome
effects on hypersensitive responsese4
Qualifier
confined to these arease4

Not reportedComparatorDirection

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e4

“When selected areas of leaves were inoculated with compatible bacteria, effects on hypersensitive responses were confined to these areas”

✓ located verbatim in the source · abstract, characters 655–791 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Incompatible bacteria inoculated through the upper leaf surface did not affect susceptible responses to compatible bacteria inoculated 24 h later.

Taxon
Beant0
Treatment
Inoculation through the upper leaf surface with incompatible bacteriae5
Outcome
susceptible responsese5
Direction
did not affecte5

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e5

“Inoculation through the upper leaf surface with incompatible bacteria did not affect susceptible responses when compatible bacteria were inoculated 24 h later through the lower surface”

✓ located verbatim in the source · abstract, characters 861–1045 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Heat-killed bacteria, or live bacteria in insufficient numbers, did not prevent development of hypersensitive responses to later incompatible bacteria.

Taxon
Beant0
Treatment
heat-killed bacteriae6
Outcome
development of these responsese6
Direction
did not prevente6

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e6

“Treatment of leaves with heat-killed bacteria or live bacteria in numbers insufficient to cause hypersensitive responses did not prevent development of these responses”

✓ located verbatim in the source · abstract, characters 1047–1214 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Heat-killed bacteria suppressed hypersensitive responses in tobacco leaves.

Treatment
Use of heat-killed bacteriae7
Outcome
hypersensitive responsese7
Direction
suppresse7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e7

“Use of heat-killed bacteria did suppress hypersensitive responses in tobacco leaves”

✓ located verbatim in the source · abstract, characters 1271–1354 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Cycloheximide (20 p.p.m.) injected 30 min before incompatible bacteria suppressed electrolyte leakage and browning associated with hypersensitive responses.

Taxon
Beant0
Treatment
cycloheximide (20 p.p.m.)e1
Interactor
incompatible bacteriae1pathogen
Outcome
leakage of electrolytes and browning of tissuese1
Direction
suppressede1
Value
20 p.p.m.e1

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e1

“Injection of leaves with cycloheximide (20 p.p.m.) 30 min before inoculation with incompatible bacteria suppressed leakage of electrolytes and browning of tissues associated with hypersensitive responses”

✓ located verbatim in the source · abstract, characters 1356–1559 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Cycloheximide had little effect on electrolyte leakage from leaves inoculated with compatible bacteria or on susceptible responses.

Taxon
Beant0
Treatment
Cycloheximidee1
Outcome
leakage of electrolytes from leaves inoculated with compatible bacteriae1
Direction
little effecte1

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e1

“Cycloheximide had little effect on leakage of electrolytes from leaves inoculated with compatible bacteria or with the development of susceptible responses”

✓ located verbatim in the source · abstract, characters 1561–1716 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Chloroform vapour exposure delayed hypersensitive responses by 24 h.

Taxon
Beant0
Treatment
Exposure of leaves to chloroform vapoure1
Outcome
hypersensitive responsese1
Direction
delayede1
Value
24 he1

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e1

“Exposure of leaves to chloroform vapour delayed hypersensitive responses by 24 h”

✓ located verbatim in the source · abstract, characters 1718–1798 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Peroxidase treatment had no effect.

Taxon
Beant0
Treatment
treatment with peroxidasee1
Outcome
effecte1
Direction
no effecte1

Not reportedComparator

Verbatim

t0

“Bean Leaves”

✓ located verbatim in the source · title, characters 26–37 · show in text ↑
e1

“treatment with peroxidase had no effect”

✓ located verbatim in the source · abstract, characters 1800–1839 · show in text ↑

inoculation · growth · Phaseolus vulgaris (common bean)Results differ

126 papers: 106 positive, 20 negative or no effect

19 papers in the same direction as this paper · 106 in the opposite direction

  1. positive
  2. positive
  3. positive

122 papers more in this group

See all 126 papers →

heat · growth · Phaseolus vulgaris (common bean)Positive and negative results

8 papers: 2 positive, 6 negative or no effect

5 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative

4 papers more in this group

See all 8 papers →

inoculation · disease · Phaseolus vulgaris (common bean)Positive and negative results

68 papers: 28 positive, 40 negative or no effect

39 papers in the same direction as this paper · 28 in the opposite direction

  1. positive
  2. positive
  3. positive

64 papers more in this group

See all 68 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.