Abstract
Aluminium is a potent toxicant in acidic soils. The present study was taken up to analyze the effects of Al on enzymes of nitrogen assimilation in excised bean (Phaseolus vulgaris) leaf segments so as to gain an insight of the mechanism involved. Supply of 0.001 to 0.1 mM AlCl3 to excised bean leaf segments affected the in vivo nitrate reductase activity differently in the presence of various inorganic nitrogenous compounds, being inhibited with 5 mM ammonium nitrate and 10 mM ammonium chloride but enhanced with 10 mM potassium nitrate. Al effect with 50 mM KNO3 varied with time, showing an increased activity at shorter duration, but decreased at longer duration. Al effect on in vivo NRA was dependent upon the nitrate concentration, thus, inhibiting it at 0, 1 and 50 mM KNO3, while increasing at 2 and 10 mM. Further, saturating and non-saturating effects were observed in the absence and presence of Al. Al supply influenced the in vitro NRA also, being increased at 10 mM, but decreased at 50 mM KNO3. Supply of Al to excised leaf segments substantially inhibited the glutamate dehydrogenase activity in the absence as well as presence of 5 mM NH4NO3 but increased the glutamate synthase activity. Inhibition of specific glutamate dehydrogenase activity by Al supply was also observed. However, specific glutamate synthase activity was increased in the presence of NH4NO3 only. The experiments demonstrated that effect of supply of aluminium on in vivo nitrate reductase activity depended upon nitrogenous source as well as nitrate concentration and it exerted reciprocal regulation of glutamate dehydrogenase and glutamate synthase activities, which depended upon N supply too.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Aluminium inhibited in vivo nitrate reductase with ammonium nitrate.
- Taxon
- beane0
- Treatment
- 0.001 to 0.1 mM AlCl3e0
- Outcome
- in vivo nitrate reductase activitye0
- Direction
- inhibitede0
- Qualifier
- 5 mM ammonium nitratee0
Not reportedComparator
Verbatim
e0“Supply of 0.001 to 0.1 mM AlCl3 to excised bean leaf segments affected the in vivo nitrate reductase activity differently in the presence of various inorganic nitrogenous compounds, being inhibited with 5 mM ammonium nitrate and 10 mM ammonium chloride but enhanced with 10 mM potassium nitrate”
Claim · model-written summary
Aluminium inhibited in vivo nitrate reductase with ammonium chloride.
- Taxon
- beane0
- Treatment
- 0.001 to 0.1 mM AlCl3e0
- Outcome
- in vivo nitrate reductase activitye0
- Direction
- inhibitede0
- Qualifier
- 10 mM ammonium chloridee0
Not reportedComparator
Verbatim
e0“Supply of 0.001 to 0.1 mM AlCl3 to excised bean leaf segments affected the in vivo nitrate reductase activity differently in the presence of various inorganic nitrogenous compounds, being inhibited with 5 mM ammonium nitrate and 10 mM ammonium chloride but enhanced with 10 mM potassium nitrate”
Claim · model-written summary
Aluminium enhanced in vivo nitrate reductase with 10 mM potassium nitrate.
- Taxon
- beane0
- Treatment
- 0.001 to 0.1 mM AlCl3e0
- Outcome
- in vivo nitrate reductase activitye0
- Direction
- enhancede0
- Qualifier
- 10 mM potassium nitratee0
Not reportedComparator
Verbatim
e0“Supply of 0.001 to 0.1 mM AlCl3 to excised bean leaf segments affected the in vivo nitrate reductase activity differently in the presence of various inorganic nitrogenous compounds, being inhibited with 5 mM ammonium nitrate and 10 mM ammonium chloride but enhanced with 10 mM potassium nitrate”
Claim · model-written summary
At 50 mM KNO3 aluminium increased activity at shorter duration.
Not reportedComparator
Verbatim
t0“Bean”
e0“Al effect with 50 mM KNO3 varied with time, showing an increased activity at shorter duration, but decreased at longer duration”
Claim · model-written summary
At 50 mM KNO3 aluminium decreased activity at longer duration.
Not reportedComparator
Verbatim
t0“Bean”
e0“Al effect with 50 mM KNO3 varied with time, showing an increased activity at shorter duration, but decreased at longer duration”
Claim · model-written summary
Aluminium inhibited in vivo nitrate reductase at 0, 1 and 50 mM KNO3.
Not reportedComparator
Verbatim
t0“Bean”
e0“Al effect on in vivo NRA was dependent upon the nitrate concentration, thus, inhibiting it at 0, 1 and 50 mM KNO3, while increasing at 2 and 10 mM”
Claim · model-written summary
Aluminium increased in vivo nitrate reductase at 2 and 10 mM KNO3.
Not reportedComparator
Verbatim
t0“Bean”
e0“Al effect on in vivo NRA was dependent upon the nitrate concentration, thus, inhibiting it at 0, 1 and 50 mM KNO3, while increasing at 2 and 10 mM”
Claim · model-written summary
Aluminium caused saturating and non-saturating effects with different presence conditions.
Verbatim
t0“Bean”
e0“saturating and non-saturating effects were observed in the absence and presence of Al”
Claim · model-written summary
Aluminium increased in vitro nitrate reductase at 10 mM KNO3.
Not reportedComparator
Verbatim
t0“Bean”
e0“Al supply influenced the in vitro NRA also, being increased at 10 mM, but decreased at 50 mM KNO3”
Claim · model-written summary
Aluminium decreased in vitro nitrate reductase at 50 mM KNO3.
Not reportedComparator
Verbatim
t0“Bean”
e0“Al supply influenced the in vitro NRA also, being increased at 10 mM, but decreased at 50 mM KNO3”
Claim · model-written summary
Aluminium inhibited glutamate dehydrogenase activity with and without ammonium nitrate.
- Taxon
- Beant0
- Treatment
- Ale0
- Outcome
- glutamate dehydrogenase activitye0
- Direction
- inhibitede0
- Qualifier
- absence as well as presence of 5 mM NH4NO3e0
Not reportedComparator
Verbatim
t0“Bean”
e0“Supply of Al to excised leaf segments substantially inhibited the glutamate dehydrogenase activity in the absence as well as presence of 5 mM NH4NO3 but increased the glutamate synthase activity”
Claim · model-written summary
Aluminium increased glutamate synthase activity.
Not reportedComparator
Verbatim
t0“Bean”
e0“Supply of Al to excised leaf segments substantially inhibited the glutamate dehydrogenase activity in the absence as well as presence of 5 mM NH4NO3 but increased the glutamate synthase activity”
Claim · model-written summary
Aluminium inhibited specific glutamate dehydrogenase activity.
- Taxon
- Beant0
- Treatment
- Al supplye0
- Outcome
- specific glutamate dehydrogenase activitye0
- Direction
- Inhibitione0
Not reportedComparator
Verbatim
t0“Bean”
e0“Inhibition of specific glutamate dehydrogenase activity by Al supply was also observed”
Claim · model-written summary
Specific glutamate synthase activity increased only with ammonium nitrate.
Not reportedComparator
Verbatim
t0“Bean”
e0“specific glutamate synthase activity was increased in the presence of NH4NO3 only”
The same questions in other papers
aluminium atom · physiology: enzyme activity · Phaseolus vulgaris (common bean)Results differ
3 in the opposite direction
- no effect
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.