Abstract
Viruses interfere with and usurp host machinery and circumvent defense responses to create a suitable cellular environment for successful infection. This is usually achieved through interactions between viral proteins and host factors. Geminiviruses are a group of plant-infecting DNA viruses, of which some contain a betasatellite, known as DNAβ. Here, we report that Cotton leaf curl Multan virus (CLCuMuV) uses its sole satellite-encoded protein βC1 to regulate the plant ubiquitination pathway for effective infection. We found that CLCuMu betasatellite (CLCuMuB) βC1 interacts with NbSKP1, and interrupts the interaction of NbSKP1s with NbCUL1. Silencing of either NbSKP1s or NbCUL1 enhances the accumulation of CLCuMuV genomic DNA and results in severe disease symptoms in plants. βC1 impairs the integrity of SCFCOI1 and the stabilization of GAI, a substrate of the SCFSYL1 to hinder responses to jasmonates (JA) and gibberellins (GA). Moreover, JA treatment reduces viral accumulation and symptoms. These results suggest that CLCuMuB βC1 inhibits the ubiquitination function of SCF E3 ligases through interacting with NbSKP1s to enhance CLCuMuV infection and symptom induction in plants.
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Claims 11
Claim · model-written summary
CLCuMuB βC1 interacts with NbSKP1.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Interactor
- CLCuMu betasatellite (CLCuMuB)e0pathogen
- Gene
- βC1e0
- Outcome
- interacts with NbSKP1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CLCuMu betasatellite (CLCuMuB) βC1 interacts with NbSKP1”
t0“Nicotiana benthamiana”
Claim · model-written summary
CLCuMuB βC1 interrupts the interaction between NbSKP1s and NbCUL1.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- CLCuMu betasatellite (CLCuMuB)e0pathogen
- Gene
- βC1e0
- Outcome
- interaction of NbSKP1s with NbCUL1e0
- Direction
- interruptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CLCuMu betasatellite (CLCuMuB) βC1 interacts with NbSKP1, and interrupts the interaction of NbSKP1s with NbCUL1”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbSKP1s enhances CLCuMuV genomic DNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of either NbSKP1s or NbCUL1e0
- Interactor
- CLCuMuVe0pathogen
- Gene
- NbSKP1se0
- Outcome
- accumulation of CLCuMuV genomic DNAe0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of either NbSKP1s or NbCUL1 enhances the accumulation of CLCuMuV genomic DNA and results in severe disease symptoms in plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbCUL1 enhances CLCuMuV genomic DNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of either NbSKP1s or NbCUL1e0
- Interactor
- CLCuMuVe0pathogen
- Gene
- NbCUL1e0
- Outcome
- accumulation of CLCuMuV genomic DNAe0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of either NbSKP1s or NbCUL1 enhances the accumulation of CLCuMuV genomic DNA and results in severe disease symptoms in plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbSKP1s resulted in severe disease symptoms.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of either NbSKP1s or NbCUL1e0
- Interactor
- CLCuMuVe0pathogen
- Gene
- NbSKP1se0
- Outcome
- severe disease symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of either NbSKP1s or NbCUL1 enhances the accumulation of CLCuMuV genomic DNA and results in severe disease symptoms in plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbCUL1 resulted in severe disease symptoms.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of either NbSKP1s or NbCUL1e0
- Interactor
- CLCuMuVe0pathogen
- Gene
- NbCUL1e0
- Outcome
- severe disease symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of either NbSKP1s or NbCUL1 enhances the accumulation of CLCuMuV genomic DNA and results in severe disease symptoms in plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
βC1 impairs SCFCOI1 integrity.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- CLCuMuBt1pathogen
- Gene
- βC1e0
- Outcome
- integrity of SCFCOI1e0
- Direction
- impairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“βC1 impairs the integrity of SCFCOI1”
t0“Nicotiana benthamiana”
t1“CLCuMuB βC1”
Claim · model-written summary
βC1 impairs GAI stabilization.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- CLCuMuBt1pathogen
- Gene
- βC1e0
- Outcome
- stabilization of GAIe0
- Direction
- impairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“βC1 impairs the integrity of SCFCOI1 and the stabilization of GAI, a substrate of the SCFSYL1 to hinder responses to jasmonates (JA) and gibberellins (GA)”
t0“Nicotiana benthamiana”
t1“CLCuMuB βC1”
Claim · model-written summary
βC1 hinders responses to jasmonates and gibberellins.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- CLCuMuBt1pathogen
- Gene
- βC1e0
- Outcome
- responses to jasmonates (JA) and gibberellins (GA)e0
- Direction
- hindere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“βC1 impairs the integrity of SCFCOI1 and the stabilization of GAI, a substrate of the SCFSYL1 to hinder responses to jasmonates (JA) and gibberellins (GA)”
t0“Nicotiana benthamiana”
t1“CLCuMuB βC1”
Claim · model-written summary
JA treatment reduces viral accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- JA treatmente0
- Interactor
- CLCuMuBt1pathogen
- Outcome
- viral accumulatione0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA treatment reduces viral accumulation and symptoms”
t0“Nicotiana benthamiana”
t1“CLCuMuB”
Claim · model-written summary
JA treatment reduces viral symptoms.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- JA treatmente0
- Interactor
- CLCuMuBt1pathogen
- Outcome
- symptomse0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA treatment reduces viral accumulation and symptoms”
t0“Nicotiana benthamiana”
t1“CLCuMuB”
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