Paper · 1965 · Biologia Plantarum

The effect of reversion and duplication of leaf disks on the accuracy of photosynthesis determination by the dry weight method

Zdenêk Šesták; Jitka Vodová

8
claims
8
verbatim quotes
1
citations
1
research areas
—
Not open access

Abstract

Experiments with sunflower and fodder cabbage showed that reversion of the leaf disks lower side upwards (towards the radiation source) decreased the photosynthetic rate by as much as 26% both at full (2. 105 erg. cm−2. sec.−1) and half density of irradiation. On placing two disks one on another the photosynthetic rate was decreased in the upper disk in a similar way as it was on reversion even if it were placed in the normal position (upper side upwards). But assimilation in the lower disk was only a fraction (0–40%) of that of the upper disk and in thick leaves was within the limits of the compensation point. The sum of the increase in dry weight in both disks due to photosynthesis exceeds the increment in one normally placed disk only in thin leaves and at high density of radiation. On the basis of the differences found it is advised to discard experimental samples where a quarter of the disks were reversed during exposure. On evaluating samples in which some of the disks were duplicated in error, it is most accurate to deduct the weight of one control disk from the weight obtained. The decrease in photosynthetic rate found in reversed and duplicated disks is evidently due to the simultaneous effect of differences in the supply of CO2 and radiation energy to the assimilating tissue and apparatus and also to differences in the photosynthetic capacity of different leaf tissues.

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Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Reversing sunflower leaf disks reduced photosynthetic rate by as much as 26% at full and half irradiation.

Taxon
sunflowerHelianthus annuuse0
Organ / stage
leaf diskse0
Treatment
reversion of the leaf disks lower side upwardse0
Outcome
photosynthetic ratee0
Direction
decreasede0
Value
26%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Experiments with sunflower and fodder cabbage showed that reversion of the leaf disks lower side upwards (towards the radiation source) decreased the photosynthetic rate by as much as 26% both at full (2. 105 erg. cm−2. sec.−1) and half density of irradiation”

✓ located verbatim in the source · abstract, characters 0–259 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Reversing fodder cabbage leaf disks reduced photosynthetic rate by as much as 26% at full and half irradiation.

Taxon
fodder cabbagee0
Organ / stage
leaf diskse0
Treatment
reversion of the leaf disks lower side upwardse0
Outcome
photosynthetic ratee0
Direction
decreasede0
Value
26%e0

Not reportedComparator

Verbatim

e0

“Experiments with sunflower and fodder cabbage showed that reversion of the leaf disks lower side upwards (towards the radiation source) decreased the photosynthetic rate by as much as 26% both at full (2. 105 erg. cm−2. sec.−1) and half density of irradiation”

✓ located verbatim in the source · abstract, characters 0–259 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Stacking two disks reduced photosynthesis in the upper disk similarly to reversal.

Organ / stage
upper diske0
Treatment
placing two disks one on anothere0
Outcome
photosynthetic ratee0
Direction
decreasede0
Comparator
reversione0

Not reportedTaxon

Verbatim

e0

“On placing two disks one on another the photosynthetic rate was decreased in the upper disk in a similar way as it was on reversion even if it were placed in the normal position (upper side upwards)”

✓ located verbatim in the source · abstract, characters 261–459 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Lower stacked disks assimilated 0–40% as much as upper disks, reaching the compensation point in thick leaves.

Outcome
assimilation in the lower diske0
Direction
only a fractione0
Value
0–40%e0
Qualifier
in thick leavese0
Comparator
upper diske0

Not reportedTaxon

Verbatim

e0

“assimilation in the lower disk was only a fraction (0–40%) of that of the upper disk and in thick leaves was within the limits of the compensation point”

✓ located verbatim in the source · abstract, characters 465–617 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Two disks exceeded one normal disk in total photosynthetic dry-weight gain only in thin leaves at high radiation.

Treatment
both diskse0
Outcome
increase in dry weighte0
Direction
exceedse0
Qualifier
only in thin leaves and at high density of radiatione0
Comparator
one normally placed diske0

Not reportedTaxon

Verbatim

e0

“The sum of the increase in dry weight in both disks due to photosynthesis exceeds the increment in one normally placed disk only in thin leaves and at high density of radiation”

✓ located verbatim in the source · abstract, characters 619–795 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors advise discarding samples when a quarter of leaf disks were reversed.

Outcome
discard experimental samples where a quarter of the disks were reversed during exposuree0

Not reportedTaxon

Verbatim

e0

“it is advised to discard experimental samples where a quarter of the disks were reversed during exposure”

✓ located verbatim in the source · abstract, characters 835–939 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

For samples with accidentally duplicated disks, the authors advise deducting one control disk weight.

Outcome
deduct the weight of one control disk from the weight obtainede0

Not reportedTaxon

Verbatim

e0

“it is most accurate to deduct the weight of one control disk from the weight obtained”

✓ located verbatim in the source · abstract, characters 1016–1101 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Differences in CO2 and radiation supply and tissue photosynthetic capacity were proposed to explain lower rates in reversed and doubled disks.

Outcome
decrease in photosynthetic ratee0
Qualifier
differences in the supply of CO2 and radiation energye0

Not reportedTaxon

Verbatim

e0

“The decrease in photosynthetic rate found in reversed and duplicated disks is evidently due to the simultaneous effect of differences in the supply of CO2 and radiation energy to the assimilating tissue and apparatus and also to differences in the photosynthetic capacity of different leaf tissues”

✓ located verbatim in the source · abstract, characters 1103–1400 · show in text ↑