Abstract
Stress-induced abscisic acid (ABA) is mainly catabolized by ABA 8'-hydroxylase (ABA8ox), which also strictly regulates endogenous ABA levels. Although three members of the ABA8ox gene family are conserved in rice, it is not clear which stressors induce expression of these genes. Here, we found that OsABA8ox1 was induced by cold stress within 24 h and that OsABA8ox2 and OsABA8ox3 were not. In contrast, OsABA8ox2 and OsABA8ox3 were ABA-inducible, but OsABA8ox1 was not. OsABA8ox1, OsABA8ox2, and OsABA8ox3 restored germination of a cyp707a1/a2/a3 triple mutant of Arabidopsis to rates comparable to those of the wild type, indicating that OsABA8ox1, OsABA8ox2, and OsABA8ox3 function as ABA-catabolic genes in vivo. Transgenic rice lines overexpressing OsABA8ox1 showed decreased levels of ABA and increased seedling vigor at 15 °C. These results indicate that sustained low levels of ABA lead to increased seedling vigor during cold stress. On the other hand, excessively low endogenous ABA levels caused reduced drought and cold tolerance, although some of the transgenic rice lines expressing OsABA8ox1 at moderate levels did not show these harmful effects. Adequate regulation of endogenous ABA levels is thought to be crucial for maintaining seedling vigor under cold stress and for cold and drought tolerance in rice.
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Claims 12
Claim · model-written summary
OsABA8ox1 was induced by cold stress within 24 h.
- Taxon
- rice
Oryza sativat0 - Treatment
- cold stresse1
- Gene
- OsABA8ox1e1
- Outcome
- OsABA8ox1e1
- Direction
- inducede1
- Qualifier
- within 24 he1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e1“OsABA8ox1 was induced by cold stress within 24 h and that OsABA8ox2 and OsABA8ox3 were not”
Claim · model-written summary
OsABA8ox2 and OsABA8ox3 were not induced by cold stress.
- Taxon
- riceOryza sativat0
- Treatment
- cold stresse1
- Gene
- OsABA8ox2 and OsABA8ox3e1
- Outcome
- OsABA8ox2 and OsABA8ox3e1
- Direction
- no_changeinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e1“OsABA8ox1 was induced by cold stress within 24 h and that OsABA8ox2 and OsABA8ox3 were not”
Claim · model-written summary
OsABA8ox2 and OsABA8ox3 were ABA-inducible.
- Taxon
- riceOryza sativat0
- Treatment
- ABAe1
- Gene
- OsABA8ox2 and OsABA8ox3e1
- Outcome
- OsABA8ox2 and OsABA8ox3e1
- Direction
- ABA-induciblee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e1“In contrast, OsABA8ox2 and OsABA8ox3 were ABA-inducible, but OsABA8ox1 was not”
Claim · model-written summary
OsABA8ox1 was not ABA-inducible.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e1“In contrast, OsABA8ox2 and OsABA8ox3 were ABA-inducible, but OsABA8ox1 was not”
Claim · model-written summary
OsABA8ox1, OsABA8ox2 and OsABA8ox3 restored germination of an Arabidopsis cyp707a1/a2/a3 triple mutant to rates comparable to wild type.
- Taxon
- Arabidopsis
Arabidopsise2 - Material
- cyp707a1/a2/a3 triple mutante2
- Treatment
- OsABA8ox1, OsABA8ox2, and OsABA8ox3e2
- Gene
- OsABA8ox1, OsABA8ox2, and OsABA8ox3e2
- Outcome
- germinatione2
- Direction
- restorede2
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“OsABA8ox1, OsABA8ox2, and OsABA8ox3 restored germination of a cyp707a1/a2/a3 triple mutant of Arabidopsis to rates comparable to those of the wild type”
Claim · model-written summary
OsABA8ox1, OsABA8ox2 and OsABA8ox3 act as ABA-catabolic genes in vivo.
- Taxon
- riceOryza sativat0
- Gene
- OsABA8ox1, OsABA8ox2, and OsABA8ox3e3
- Outcome
- ABA-catabolic genes in vivoe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e3“indicating that OsABA8ox1, OsABA8ox2, and OsABA8ox3 function as ABA-catabolic genes in vivo”
Claim · model-written summary
Overexpression of OsABA8ox1 in transgenic rice lowered ABA levels.
- Taxon
- riceOryza sativat0
- Material
- Transgenic rice lines overexpressing OsABA8ox1e4
- Treatment
- overexpressing OsABA8ox1e4
- Compound
- ABAe4
- Outcome
- levels of ABAe4
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e4“Transgenic rice lines overexpressing OsABA8ox1 showed decreased levels of ABA and increased seedling vigor at 15 °C”
Claim · model-written summary
Overexpression of OsABA8ox1 increased seedling vigor at 15 °C.
- Taxon
- riceOryza sativat0
- Material
- Transgenic rice lines overexpressing OsABA8ox1e4
- Treatment
- overexpressing OsABA8ox1e4
- Outcome
- seedling vigore4
- Direction
- increasede4
- Qualifier
- at 15 °Ce4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e4“Transgenic rice lines overexpressing OsABA8ox1 showed decreased levels of ABA and increased seedling vigor at 15 °C”
Claim · model-written summary
Sustained low ABA levels lead to increased seedling vigor during cold stress.
- Taxon
- riceOryza sativat0
- Compound
- ABAe5
- Outcome
- seedling vigore5
- Qualifier
- during cold stresse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e5“These results indicate that sustained low levels of ABA lead to increased seedling vigor during cold stress”
Claim · model-written summary
Excessively low endogenous ABA levels reduced drought and cold tolerance.
- Taxon
- riceOryza sativat0
- Treatment
- excessively low endogenous ABA levelse6
- Outcome
- drought and cold tolerancee6
- Direction
- reducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e6“excessively low endogenous ABA levels caused reduced drought and cold tolerance”
Claim · model-written summary
Some transgenic rice lines expressing OsABA8ox1 at moderate levels did not show the harmful effects.
- Taxon
- riceOryza sativat0
- Material
- transgenic rice lines expressing OsABA8ox1 at moderate levelse7
- Treatment
- expressing OsABA8ox1 at moderate levelse7
- Outcome
- harmful effectse7
- Direction
- did not showe7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e7“some of the transgenic rice lines expressing OsABA8ox1 at moderate levels did not show these harmful effects”
Claim · model-written summary
Adequate regulation of endogenous ABA levels is thought to be crucial for maintaining seedling vigor under cold stress.
- Taxon
- riceOryza sativae7
- Outcome
- maintaining seedling vigor under cold stresse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)”
e7“Adequate regulation of endogenous ABA levels is thought to be crucial for maintaining seedling vigor under cold stress and for cold and drought tolerance in rice”
The same questions in other papers
(+)-abscisic acid · tolerance: abiotic · Oryza sativa (rice)Results differ
1 paper in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
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