Paper · 2015 · Legume Research - An International Journal

Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings

Suruchi M. Khanna; Pooja C. Taxak; Pradeep K. Jain; et al.

6
claims
12
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

The effect of dehydration on the specific activities and transcript levels of several glycolytic enzymes was determined in two chickpea (Cicer arietinum L.) cultivars- Pusa362 (drought tolerant) and SBD377 (drought sensitive). Dehydration treatment was imposed on 6 days old chickpea seedlings by removing them from pots and keeping them on table top on 3MM Whatman paper at room temperature for 5 h. The stress imposed was judged by determining relative water content (RWC) and proline content. RWC decreased and proline content accumulated after five hours of dehydration treatment. The decline in RWC was higher in SBD377 than in Pusa362, while varietal differences were absent in proline content. With the exception of NADP-glyceraldehyde-3-phospahte dehydrogense, whose activity as well as transcript level showed marginal decrease in both the varieties, dehydration had little effect on all glycolytic enzymes studied.

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Claims 6

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Dehydration decreased relative water content of chickpea seedlings after five hours.

Taxon
chickpeaCicer arietinume1
Organ / stage
6 days old chickpea seedlingse1
Treatment
dehydration treatmente2
Outcome
RWCe2
Direction
decreasede2
Qualifier
after five hourse2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Dehydration treatment was imposed on 6 days old chickpea seedlings”

✓ located verbatim in the source · abstract, characters 227–293 · show in text ↑
e2

“RWC decreased and proline content accumulated after five hours of dehydration treatment”

✓ located verbatim in the source · abstract, characters 496–583 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Dehydration led to proline accumulation in chickpea seedlings after five hours.

Taxon
chickpeaCicer arietinume1
Organ / stage
6 days old chickpea seedlingse1
Treatment
dehydration treatmente2
Outcome
proline contente2
Direction
accumulatede2
Qualifier
after five hourse2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Dehydration treatment was imposed on 6 days old chickpea seedlings”

✓ located verbatim in the source · abstract, characters 227–293 · show in text ↑
e2

“RWC decreased and proline content accumulated after five hours of dehydration treatment”

✓ located verbatim in the source · abstract, characters 496–583 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

The decline in relative water content was higher in the drought-sensitive cultivar SBD377 than in Pusa362.

Taxon
chickpeaCicer arietinume1
Material
SBD377e3
Outcome
decline in RWCe3
Direction
highere3
Comparator
Pusa362e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Dehydration treatment was imposed on 6 days old chickpea seedlings”

✓ located verbatim in the source · abstract, characters 227–293 · show in text ↑
e3

“The decline in RWC was higher in SBD377 than in Pusa362, while varietal differences were absent in proline content”

✓ located verbatim in the source · abstract, characters 585–699 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Proline content showed no varietal difference between SBD377 and Pusa362 chickpea seedlings.

Taxon
chickpeaCicer arietinume1
Organ / stage
6 days old chickpea seedlingse1
Outcome
proline contente3
Direction
no_changeinferrede3
Qualifier
varietal differences were absente3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Dehydration treatment was imposed on 6 days old chickpea seedlings”

✓ located verbatim in the source · abstract, characters 227–293 · show in text ↑
e3

“The decline in RWC was higher in SBD377 than in Pusa362, while varietal differences were absent in proline content”

✓ located verbatim in the source · abstract, characters 585–699 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Dehydration showed a marginal decrease in NADP-glyceraldehyde-3-phosphate dehydrogenase activity and transcript level in both chickpea cultivars.

Taxon
chickpeaCicer arietinume1
Treatment
dehydratione5
Gene
NADP-glyceraldehyde-3-phospahte dehydrogensee5
Outcome
activity as well as transcript levele5
Direction
marginal decreasee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Dehydration treatment was imposed on 6 days old chickpea seedlings”

✓ located verbatim in the source · abstract, characters 227–293 · show in text ↑
e5

“With the exception of NADP-glyceraldehyde-3-phospahte dehydrogense, whose activity as well as transcript level showed marginal decrease in both the varieties, dehydration had little effect on all glycolytic enzymes studied”

✓ located verbatim in the source · abstract, characters 701–923 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Dehydration had little effect on the other glycolytic enzymes studied in chickpea.

Taxon
chickpeaCicer arietinume1
Treatment
dehydratione5
Outcome
glycolytic enzymese5
Direction
little effecte5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Dehydration treatment was imposed on 6 days old chickpea seedlings”

✓ located verbatim in the source · abstract, characters 227–293 · show in text ↑
e5

“With the exception of NADP-glyceraldehyde-3-phospahte dehydrogense, whose activity as well as transcript level showed marginal decrease in both the varieties, dehydration had little effect on all glycolytic enzymes studied”

✓ located verbatim in the source · abstract, characters 701–923 · show in text ↑

drought · physiology: water · Cicer arietinum (chickpea)Positive and negative results

21 papers: 2 positive, 19 negative or no effect

18 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative

17 papers more in this group

See all 21 papers →

drought · physiology: enzyme activity · Cicer arietinum (chickpea)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.