Abstract
Aerial plant surfaces are coated by a cuticular wax layer to protect against environmental stresses, such as desiccation. In this study, we investigated the functional relationship between MYB94 and MYB96 transcription factors involved in cuticular wax biosynthesis. Both MYB94 and MYB96 transcripts were abundantly expressed in the aerial organs of Arabidopsis, and significantly induced at the same or similar time points under conditions of drought. MYB94 complemented the wax-deficient phenotype of the myb96 loss-of-function mutant under both well-watered and drought stress conditions. The magnitude of decrease in total wax load in the myb94 myb96 double mutant was almost equal to the sum of the reduced wax loads in the individual myb94 and myb96 mutants under both conditions. Leaves of the myb94 myb96 mutant lost water through the cuticle faster than those of myb94 or myb96 plants. Transcript levels of wax biosynthetic genes were decreased in the single mutants, and further reduced in the double mutant, relative to the wild type, under drought and ABA treatment conditions. MYB94 and MYB96 interact with the same regions containing MYB consensus motifs in the promoter regions of wax biosynthetic genes. The data collectively indicate that MYB94 and MYB96 exert an additive effect on cuticular wax biosynthesis, which may represent an efficient adaptive mechanism of response to drought in plants.
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Claims 9
Claim · model-written summary
MYB94 and MYB96 transcripts were abundantly expressed in the aerial organs of Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- aerial organse0
- Gene
- MYB94 and MYB96e0
- Outcome
- abundantly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both MYB94 and MYB96 transcripts were abundantly expressed in the aerial organs of Arabidopsis”
Claim · model-written summary
MYB94 and MYB96 transcripts were significantly induced at the same or similar time points under drought in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- droughte0
- Gene
- MYB94 and MYB96e0
- Outcome
- significantly induced at the same or similar time pointse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both MYB94 and MYB96 transcripts were abundantly expressed in the aerial organs of Arabidopsis, and significantly induced at the same or similar time points under conditions of drought”
Claim · model-written summary
MYB94 complemented the wax-deficient phenotype of the myb96 loss-of-function mutant under both well-watered and drought stress conditions.
- Taxon
- ArabidopsisArabidopsist0
- Material
- myb96 loss-of-function mutante0
- Gene
- MYB94e0
- Outcome
- wax-deficient phenotypee0
- Qualifier
- under both well-watered and drought stress conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB94 complemented the wax-deficient phenotype of the myb96 loss-of-function mutant under both well-watered and drought stress conditions”
t0“Arabidopsis”
Claim · model-written summary
The decrease in total wax load in the myb94 myb96 double mutant was almost equal to the sum of the reduced wax loads in the individual myb94 and myb96 mutants under both conditions.
- Taxon
- ArabidopsisArabidopsist0
- Material
- myb94 myb96 double mutante0
- Outcome
- total wax loade0
- Direction
- decreasee0
- Comparator
- individual myb94 and myb96 mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The magnitude of decrease in total wax load in the myb94 myb96 double mutant was almost equal to the sum of the reduced wax loads in the individual myb94 and myb96 mutants under both conditions”
t0“Arabidopsis”
Claim · model-written summary
Leaves of the myb94 myb96 mutant lost water through the cuticle faster than those of myb94 or myb96 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- myb94 myb96 mutante0
- Organ / stage
- Leavese0
- Outcome
- lost water through the cuticlee0
- Direction
- fastere0
- Comparator
- myb94 or myb96 plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaves of the myb94 myb96 mutant lost water through the cuticle faster than those of myb94 or myb96 plants”
t0“Arabidopsis”
Claim · model-written summary
Transcript levels of wax biosynthetic genes were decreased in the myb94 and myb96 single mutants relative to the wild type under drought and ABA treatment.
- Taxon
- ArabidopsisArabidopsist0
- Material
- single mutantse0
- Treatment
- drought and ABA treatment conditionse0
- Gene
- wax biosynthetic genese0
- Outcome
- Transcript levels of wax biosynthetic genese0
- Direction
- decreasede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of wax biosynthetic genes were decreased in the single mutants, and further reduced in the double mutant, relative to the wild type, under drought and ABA treatment conditions”
t0“Arabidopsis”
Claim · model-written summary
Transcript levels of wax biosynthetic genes were further reduced in the myb94 myb96 double mutant relative to the wild type under drought and ABA treatment.
- Taxon
- ArabidopsisArabidopsist0
- Material
- double mutante0
- Treatment
- drought and ABA treatment conditionse0
- Gene
- wax biosynthetic genese0
- Outcome
- Transcript levels of wax biosynthetic genese0
- Direction
- further reducede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of wax biosynthetic genes were decreased in the single mutants, and further reduced in the double mutant, relative to the wild type, under drought and ABA treatment conditions”
t0“Arabidopsis”
Claim · model-written summary
MYB94 and MYB96 interact with the same regions containing MYB consensus motifs in the promoter regions of wax biosynthetic genes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB94 and MYB96e0
- Outcome
- interact with the same regions containing MYB consensus motifs in the promoter regions of wax biosynthetic genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB94 and MYB96 interact with the same regions containing MYB consensus motifs in the promoter regions of wax biosynthetic genes”
t0“Arabidopsis”
Claim · model-written summary
The authors conclude that MYB94 and MYB96 exert an additive effect on cuticular wax biosynthesis in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB94 and MYB96e0
- Outcome
- cuticular wax biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB94 and MYB96 exert an additive effect on cuticular wax biosynthesis”
t0“Arabidopsis”