Abstract
Vinblastine and vincristine are secondary metabolites from Madagascar periwinkles that have a very high economic value as chemotherapy drugs. These compounds are naturally produced in a very low quantity in planta. One promising alternative method for vinblastine and vincristine production is to use a treatment that can trigger plant stress response in vitro. This study has been done to evaluate the effect of drought stress using polyethylene glycol (PEG) on vinblastine and vincristine production in the C. roseus callus culture, which were grown on medium Zenk supplemented with plant growth regulators (PGR) 1 μM NAA + 10 μM Kinetin to induce laticifer and idioblast differentiation. 13-week-old callus cultures were then treated with 0%, 6%, 9%, and 12% (w/v) PEG4000 each for 0, 24, 48, and 72 hours. Biochemical analysis was performed using HPLC to determine the levels of vinblastine and vincristine, while the presence of differentiated cells (idioblasts and laticifers) was determined using a histochemical method. Protein profiles of the culture were determined by SDS-Page. The results showed that drought treatment with PEG4000, until the concentration was 12% (w/v), did not significantly affect the production of vinblastine and vincristine, but might affect terpenoid production. Histochemical analysis confirmed the presence of idioblasts, non-elongated laticifers, and laticifers that were producing and accumulating terpenoids highest in the 12% PEG treatment. PEG treatments also did not change the protein profile of callus.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
PEG4000 drought treatment up to 12% did not significantly affect vinblastine production in periwinkle callus.
- Taxon
- Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)
Catharanthus roseust0 - Treatment
- drought treatment with PEG4000e0
- Outcome
- production of vinblastinee0
- Direction
- did not significantly affecte0
- Qualifier
- until the concentration was 12% (w/v)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that drought treatment with PEG4000, until the concentration was 12% (w/v), did not significantly affect the production of vinblastine and vincristine, but might affect terpenoid production.”
t0“Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)”
Claim · model-written summary
PEG4000 drought treatment up to 12% did not significantly affect vincristine production in periwinkle callus.
- Taxon
- Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)Catharanthus roseust0
- Treatment
- drought treatment with PEG4000e0
- Outcome
- vincristinee0
- Direction
- did not significantly affecte0
- Qualifier
- until the concentration was 12% (w/v)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that drought treatment with PEG4000, until the concentration was 12% (w/v), did not significantly affect the production of vinblastine and vincristine, but might affect terpenoid production.”
t0“Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)”
Claim · model-written summary
PEG4000 drought treatment might affect terpenoid production in periwinkle callus.
- Taxon
- Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)Catharanthus roseust0
- Treatment
- drought treatment with PEG4000e0
- Outcome
- terpenoid productione0
- Qualifier
- might affecte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that drought treatment with PEG4000, until the concentration was 12% (w/v), did not significantly affect the production of vinblastine and vincristine, but might affect terpenoid production.”
t0“Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)”
Claim · model-written summary
Idioblasts were present in periwinkle callus.
- Taxon
- Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)Catharanthus roseust0
- Organ / stage
- idioblastse0
- Outcome
- presence of idioblastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histochemical analysis confirmed the presence of idioblasts, non-elongated laticifers, and laticifers that were producing and accumulating terpenoids highest in the 12% PEG treatment.”
t0“Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)”
Claim · model-written summary
Non-elongated laticifers were present in periwinkle callus.
- Taxon
- Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)Catharanthus roseust0
- Organ / stage
- laticiferse0
- Outcome
- non-elongated laticiferse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histochemical analysis confirmed the presence of idioblasts, non-elongated laticifers, and laticifers that were producing and accumulating terpenoids highest in the 12% PEG treatment.”
t0“Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)”
Claim · model-written summary
Laticifers produced and accumulated the most terpenoids under 12% PEG treatment.
- Taxon
- Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)Catharanthus roseust0
- Organ / stage
- laticiferse0
- Treatment
- 12% PEG treatmente0
- Outcome
- laticifers that were producing and accumulating terpenoidse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histochemical analysis confirmed the presence of idioblasts, non-elongated laticifers, and laticifers that were producing and accumulating terpenoids highest in the 12% PEG treatment.”
t0“Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)”
Claim · model-written summary
PEG treatments did not change the protein profile of periwinkle callus.
- Taxon
- Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)Catharanthus roseust0
- Treatment
- PEG treatmentse0
- Outcome
- protein profile of calluse0
- Direction
- did not changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PEG treatments also did not change the protein profile of callus.”
t0“Madagascar Periwinkle (Catharanthus roseus (L.) G. Don)”
The same questions in other papers
drought · molecular: metabolites · Catharanthus roseus (Madagascar periwinkle)Results differ
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →drought · yield · Catharanthus roseus (Madagascar periwinkle)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.