Abstract
Flooding stress has a negative impact on soybean cultivation because it severely impairs growth and development. To understand the flooding responsive mechanism in early stage soybeans, a glycoproteomic technique was used. Two-day-old soybeans were treated with flooding for 2 days and roots were collected. Globally, the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress. Glycoproteins were enriched from total protein extracts using concanavalin A lectin resin and analyzed using a gel-free proteomic technique. One-hundred eleven and 69 glycoproteins were identified without and with 2 days of flooding stress, respectively. Functional categorization of these identified glycoproteins indicated that the accumulation level of proteins related to protein degradation, cell wall, and glycolysis increased, while stress-related proteins decreased under flooding stress. Also the accumulation level of glycoproteins localized in the secretory pathway decreased under flooding stress. Out of 23 common glycoproteins between control and flooding conditions, peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress. mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteins were downregulated by flooding stress. These results suggest that flooding might negatively affect the process of N-glycosylation of proteins related to stress and protein degradation; however glycoproteins involved in glycolysis are activated.
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Claims 10
Claim · model-written summary
Total glycoprotein accumulation decreased after two days of flooding.
- Taxon
- soybean
Glycine maxt0 - Treatment
- 2 days of flooding stresse0
- Outcome
- accumulation level of glycoproteinse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Globally, the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress.”
t0“soybean”
Claim · model-written summary
The number of identified glycoproteins was 111 without flooding and 69 after two days of flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 2 days of flooding stresse0
- Outcome
- glycoproteins were identifiede0
- Direction
- decreasede1
- Value
- 69e0
- Comparator
- withoute0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One-hundred eleven and 69 glycoproteins were identified without and with 2 days of flooding stress, respectively.”
t0“soybean”
e1“Globally, the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress.”
Claim · model-written summary
Protein-degradation, cell-wall, and glycolysis-related glycoproteins increased under flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- accumulation level of proteins related to protein degradation, cell wall, and glycolysise0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional categorization of these identified glycoproteins indicated that the accumulation level of proteins related to protein degradation, cell wall, and glycolysis increased, while stress-related proteins decreased under flooding stress.”
t0“soybean”
Claim · model-written summary
Stress-related glycoproteins decreased under flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- stress-related proteinse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress-related proteins decreased under flooding stress”
t0“soybean”
Claim · model-written summary
Secretory-pathway glycoproteins decreased under flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- accumulation level of glycoproteins localized in the secretory pathwaye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation level of glycoproteins localized in the secretory pathway decreased under flooding stress”
t0“soybean”
Claim · model-written summary
Peroxidases decreased after two days of flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 2 days of flooding stresse0
- Outcome
- peroxidasese0
- Direction
- decreasede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Out of 23 common glycoproteins between control and flooding conditions, peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress.”
t0“soybean”
Claim · model-written summary
Glycosyl hydrolases decreased after two days of flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 2 days of flooding stresse0
- Outcome
- glycosyl hydrolasese0
- Direction
- decreasede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Out of 23 common glycoproteins between control and flooding conditions, peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress.”
t0“soybean”
Claim · model-written summary
Transcripts for endoplasmic-reticulum and N-glycosylation-related proteins were downregulated by flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteinse0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteins were downregulated by flooding stress.”
t0“soybean”
Claim · model-written summary
The authors infer flooding may impair N-glycosylation of stress- and degradation-related proteins.
- Taxon
- soybeanGlycine maxt0
- Treatment
- floodinge0
- Outcome
- process of N-glycosylation of proteins related to stress and protein degradatione0
- Direction
- negatively affecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that flooding might negatively affect the process of N-glycosylation of proteins related to stress and protein degradation; however glycoproteins involved in glycolysis are activated.”
t0“soybean”
Claim · model-written summary
The authors infer that glycolysis-related glycoproteins are activated under flooding.
- Taxon
- soybeanGlycine maxt0
- Outcome
- glycoproteins involved in glycolysise0
- Direction
- activatede0
- Qualifier
- glycolysise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glycoproteins involved in glycolysis are activated”
t0“soybean”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.