Abstract
Lettuce is an important edible crop which possesses various medicinal properties. In this study Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene. Transgene integration and expression was confirmed through PCR and semiquantitative RT-PCR. The transformed extracts were evaluated for their in vitro antioxidant and in vivo analgesic, anti-inflammatory and antidepressant activities in rats. The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants. Results of total reducing power and total antioxidant capacity exhibited 90-118 and 61-75 % increase in transformed plants, respectively. In contrast to control, DPPH, lipid peroxidation and DNA protection assay showed up to 37, 20 and 50 % enhancement in transformed plants, respectively. The extracts showed similar but significant enhancement behavior in hot plate analgesic and carrageenan-induced hind paw edema test. The transformed extracts showed 72.1 and 78.5 % increase for analgesic and anti-inflammatory activities, respectively. The transformants of rol C gene exhibited prominent antidepressant activity with 64-73 % increase compared with untransformed plants. In conclusion, the present work suggests that transformation with rol C gene can be used to generate lettuce with enhanced medicinally important properties, such as antioxidant, analgesic, anti-inflammatory and antidepressant potential.
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Claims 11
Claim · model-written summary
The rol C transformed lettuce showed a 53-98 % increase in total phenolic content compared with untransformed plants.
- Taxon
- Lactuca sativa L.
Lactuca sativae0 - Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- total phenolice1
- Direction
- increasee1
- Value
- 53-98 %e1
- Comparator
- untransformed plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e1“The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants”
Claim · model-written summary
The rol C transformed lettuce showed a 45-58 % increase in total flavonoid content compared with untransformed plants.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- total flavonoid contentse1
- Direction
- increasee1
- Value
- 45-58 %e1
- Comparator
- untransformed plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e1“The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants”
Claim · model-written summary
The rol C transformed lettuce showed a 90-118 % increase in total reducing power.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- total reducing powere2
- Direction
- increasee2
- Value
- 90-118e2
- Comparator
- untransformed plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e2“Results of total reducing power and total antioxidant capacity exhibited 90-118 and 61-75 % increase in transformed plants, respectively”
e1“The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants”
Claim · model-written summary
The rol C transformed lettuce showed a 61-75 % increase in total antioxidant capacity.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- total antioxidant capacitye2
- Direction
- increasee2
- Value
- 61-75e2
- Comparator
- untransformed plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e2“Results of total reducing power and total antioxidant capacity exhibited 90-118 and 61-75 % increase in transformed plants, respectively”
e1“The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants”
Claim · model-written summary
The rol C transformed lettuce showed up to 37 % enhancement in DPPH activity compared with control.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- DPPHe3
- Direction
- enhancemente3
- Value
- 37e3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e3“In contrast to control, DPPH, lipid peroxidation and DNA protection assay showed up to 37, 20 and 50 % enhancement in transformed plants, respectively”
Claim · model-written summary
The rol C transformed lettuce showed up to 20 % enhancement in lipid peroxidation inhibition compared with control.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- lipid peroxidatione3
- Direction
- enhancemente3
- Value
- 20e3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e3“In contrast to control, DPPH, lipid peroxidation and DNA protection assay showed up to 37, 20 and 50 % enhancement in transformed plants, respectively”
Claim · model-written summary
The rol C transformed lettuce showed up to 50 % enhancement in DNA protection compared with control.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- DNA protection assaye3
- Direction
- enhancemente3
- Value
- 50 %e3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e3“In contrast to control, DPPH, lipid peroxidation and DNA protection assay showed up to 37, 20 and 50 % enhancement in transformed plants, respectively”
Claim · model-written summary
Extracts of rol C transformed lettuce showed similar but significant enhancement in hot plate analgesic and carrageenan-induced hind paw edema tests.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- hot plate analgesic and carrageenan-induced hind paw edema teste4
- Direction
- enhancemente4
- Qualifier
- similar but significante4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e4“The extracts showed similar but significant enhancement behavior in hot plate analgesic and carrageenan-induced hind paw edema test”
Claim · model-written summary
Extracts of rol C transformed lettuce showed a 72.1 % increase in analgesic activity.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- analgesice5
- Direction
- increasee5
- Value
- 72.1e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e5“The transformed extracts showed 72.1 and 78.5 % increase for analgesic and anti-inflammatory activities, respectively”
Claim · model-written summary
Extracts of rol C transformed lettuce showed a 78.5 % increase in anti-inflammatory activity.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- anti-inflammatorye5
- Direction
- increasee5
- Value
- 78.5e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e5“The transformed extracts showed 72.1 and 78.5 % increase for analgesic and anti-inflammatory activities, respectively”
Claim · model-written summary
Transformants of rol C gene showed a 64-73 % increase in antidepressant activity compared with untransformed plants.
- Taxon
- Lactuca sativa L.Lactuca sativae0
- Material
- cv Grand Rapidse0
- Treatment
- Agrobacterium-mediated transformation with rol C genee0
- Outcome
- antidepressant activitye6
- Direction
- increasee6
- Value
- 64-73 %e6
- Comparator
- untransformed plantse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene”
e6“The transformants of rol C gene exhibited prominent antidepressant activity with 64-73 % increase compared with untransformed plants”
The same questions in other papers
transgenic · molecular: metabolites · Lactuca sativa (garden lettuce)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.