Abstract
The pleiotropic drug resistance (PDR) sub-family of adenosine triphosphate (ATP)-binding cassette (ABC) transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with AePDR7 and BdPDR7. Subcellular localization analysis showed that TaPDR7 was expressed on the plasmalemma. The quantitative real time PCR (RT-PCR) analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol (DON) in spike. When knocking down the transcripts of all TaPDR7 members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that TaPDR7 might play an important role in response to F. graminearum. Although salicylic acid (SA), methyl jasmonate (MeJA) and abscisic acid (ABA) had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of TaPDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid (IAA).
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Claims 8
Claim · model-written summary
Three novel PDR genes were cloned from the FHB-resistant wheat cultivar Ning 7840 and located on wheat chromosomes 6A, 6B and 6D.
- Taxon
- wheat
Triticum aestivume1 - Material
- Ning 7840e1
- Gene
- PDR genese2
- Outcome
- located on wheat chromosomese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e2“we cloned three novel PDR genes in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D”
Claim · model-written summary
The three PDR genes are members of phylogenetic cluster I together with AePDR7 and BdPDR7.
- Taxon
- wheatTriticum aestivume1
- Gene
- AePDR7e3
- Outcome
- members of cluster Ie3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e3“these genes were members of cluster I together with AePDR7 and BdPDR7”
Claim · model-written summary
TaPDR7 is expressed on the plasmalemma.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaPDR7e4
- Outcome
- Subcellular localizatione4
- Qualifier
- expressed on the plasmalemmae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e4“Subcellular localization analysis showed that TaPDR7 was expressed on the plasmalemma”
Claim · model-written summary
TaPDR7 and its probable orthologues were sharply up-regulated in spikes 48 h after F. graminearum and DON infection.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- spikee5
- Treatment
- infected by Fusarium graminearum and trichothecene deoxynivalenol (DON)e5
- Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume5pathogen - Outcome
- this gene and its probable orthologuese5
- Direction
- up-regulatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e5“this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol (DON) in spike”
Claim · model-written summary
Knocking down all TaPDR7 members promoted F. graminearum hyphae growth in Ning 7840.
- Taxon
- wheatTriticum aestivume1
- Treatment
- knocking down the transcripts of all TaPDR7 members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) systeme6
- Interactor
- F. graminearumFusarium graminearume6pathogen
- Outcome
- F. graminearum hyphae growthe6
- Direction
- promotee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e6“When knocking down the transcripts of all TaPDR7 members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840”
Claim · model-written summary
Knocking down all TaPDR7 members produced larger pathogen inoculation points in Ning 7840.
- Taxon
- wheatTriticum aestivume1
- Treatment
- knocking down the transcripts of all TaPDR7 members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) systeme6
- Interactor
- F. graminearumFusarium graminearume6pathogen
- Outcome
- pathogen inoculation pointse6
- Direction
- made largere6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e6“When knocking down the transcripts of all TaPDR7 members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840”
Claim · model-written summary
Salicylic acid, methyl jasmonate and abscisic acid negatively regulate the three TaPDR7 members.
- Taxon
- wheatTriticum aestivume1
- Treatment
- salicylic acid (SA), methyl jasmonate (MeJA) and abscisic acid (ABA)e7
- Gene
- TaPDR7e3
- Outcome
- the three members of TaPDR7e3
- Direction
- negatively regulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e7“Although salicylic acid (SA), methyl jasmonate (MeJA) and abscisic acid (ABA) had been reported to possibly regulate wheat FHB resistance”
e3“here, we found that the three members of TaPDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid (IAA)”
Claim · model-written summary
Indoleacetic acid positively regulates the three TaPDR7 members.
- Taxon
- wheatTriticum aestivume1
- Treatment
- indoleacetic acid (IAA)e3
- Gene
- TaPDR7e3
- Outcome
- the three members of TaPDR7e3
- Direction
- positively regulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840”
e3“here, we found that the three members of TaPDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid (IAA)”
The same questions in other papers
loss of function · Fusarium graminearum · Triticum aestivum (wheat)Positive and negative results
20 papers in the same direction as this paper · 40 in the opposite direction
- negative
- positive
- positive
57 papers more in this group
See all 61 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.