Abstract
Elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbNAC1 protein localized to the cell nucleus. GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. Functional analysis showed that cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method. GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type. Thus, GbNAC1 is involved in the positive regulation of resistance to Verticillium wilt. In addition, analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development, and response to various abiotic stresses (ABA, mannitol, and NaCl). This suggests that GbNAC1 plays an important role in resistance to biotic and abiotic stresses in cotton. This study provides a foundation for further study of the function of NAC genes in cotton and other plants.
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Claims 8
Claim · model-written summary
GbNAC1 was identified in Gossypium barbadense L.
- Taxon
- Gossypium barbadense L.
.jpg?width=60)
Gossypium barbadensee0 - Gene
- GbNAC1e0
- Outcome
- GbNAC1, was identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L.”
Claim · model-written summary
GbNAC1 belongs to the TERN subgroup.
- Taxon
- Cotton
Gossypium hirsutumt0 - Gene
- GbNAC1e0
- Outcome
- belongs to the TERN subgroupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GbNAC1 belongs to the TERN subgroup”
t0“Cotton”
Claim · model-written summary
GbNAC1 protein localized to the cell nucleus.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- GbNAC1e0
- Outcome
- localized to the cell nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GbNAC1 protein localized to the cell nucleus”
t0“Cotton”
Claim · model-written summary
GbNAC1 was expressed in roots, stems and leaves, especially in vascular bundles.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- roots, stems, and leavese0
- Gene
- GbNAC1e0
- Outcome
- especially highly expressed in vascular bundlese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles”
t0“Cotton”
Claim · model-written summary
Silencing GbNAC1 reduced cotton resistance to Verticillium wilt.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GbNAC1 gene was silencede0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GbNAC1e0
- Outcome
- resistance to Verticillium wilte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method”
Claim · model-written summary
GbNAC1-overexpressing Arabidopsis had enhanced resistance to Verticillium dahliae versus wild type.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- GbNAC1-overexpressinge0
- Interactor
- Verticillium dahliae
Verticillium dahliaee0pathogen - Gene
- GbNAC1e0
- Outcome
- resistance to Verticillium dahliaee0
- Direction
- enhancede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type”
Claim · model-written summary
GbNAC1 overexpression was implicated in plant growth and development under stress treatments.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- GbNAC1e0
- Outcome
- plant growth, developmente0
- Qualifier
- under different stress treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development”
Claim · model-written summary
GbNAC1 overexpression was implicated in response to ABA, mannitol and NaCl.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- response to various abiotic stressese0
- Qualifier
- ABA, mannitol, and NaCle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“response to various abiotic stresses (ABA, mannitol, and NaCl)”
t0“GbNAC1-overexpressing Arabidopsis under different stress treatments”
The same questions in other papers
loss of function · Verticillium · Gossypium hirsutum (cotton)Positive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.