Abstract
Verticillium wilt will seriously affect cotton yield and fiber quality. BEL1-Like transcription factors are involved in the regulation of secondary cell wall (SCW) formation, especially the biosynthesis of lignin that also plays a key role in cotton disease resistance. However, there is no report on the role of BEL1-Like transcription factor in the regulation of plant biological stress. In this study, tissue expression pattern analysis showed that a BEL1-Like transcription factor GhBLH7-D06 was predominantly expressed in vascular tissues and the SCW thickening stage of fiber development, while its expression could also respond to Verticillium dahliae infection and the phytohormone MeJA treatment, which indicated that GhBLH7-D06 might be involved in the defense response of Verticillium wilt. Using virus-induced gene silencing (VIGS) technology, we found silencing the expression of GhBLH7-D06 could enhance the resistance of cotton plants to Verticillium wilt, and the acquisition of resistance might be mainly due to the significant overexpression of genes related to lignin biosynthesis and JA signaling pathway, which also proves that GhBLH7-D06 negatively regulates the resistance of cotton to Verticillium wilt. Based on the results of yeast two-hybrid (Y2H) library screening and confirmation by bimolecular fluorescence complementary (BiFC) experiment, we found an Ovate Family Protein (OFP) transcription factor GhOFP3-D13 which was also a negative regulator of cotton Verticillium wilt resistance could that interacts with GhBLH7-D06. Furthermore, the dual-luciferase reporter assay and yeast one-hybrid (Y1H) experiment indicated that GhBLH7-D06 could target binding to the promoter region of GhPAL-A06 to suppress its expression and eventually lead to the inhibition of lignin biosynthesis. In general, the GhBLH7-D06/GhOFP3-D13 complex can negatively regulate resistance to Verticillium wilt of cotton by inhibiting lignin biosynthesis and JA signaling pathway.
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Claims 11
Claim · model-written summary
GhBLH7-D06 was predominantly expressed in vascular tissues and the fiber secondary wall thickening stage.
- Taxon
- Cotton
Gossypium hirsutumt0 - Organ / stage
- vascular tissues and the SCW thickening stage of fiber developmente0
- Gene
- GhBLH7-D06e0
- Outcome
- predominantly expressede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a BEL1-Like transcription factor GhBLH7-D06 was predominantly expressed in vascular tissues and the SCW thickening stage of fiber development”
t0“Cotton”
Claim · model-written summary
GhBLH7-D06 expression responded to Verticillium dahliae infection.
- Taxon
- CottonGossypium hirsutumt0
- Interactor
- Verticillium dahliae
Verticillium dahliaee0pathogen - Gene
- GhBLH7-D06t0
- Outcome
- expression could also responde0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression could also respond to Verticillium dahliae infection and the phytohormone MeJA treatment”
t0“Cotton BEL1-Like Transcription Factor GhBLH7-D06”
Claim · model-written summary
GhBLH7-D06 expression responded to MeJA treatment.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- MeJA treatmente0
- Gene
- GhBLH7-D06t0
- Outcome
- expression could also responde0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression could also respond to Verticillium dahliae infection and the phytohormone MeJA treatment”
t0“Cotton BEL1-Like Transcription Factor GhBLH7-D06”
Claim · model-written summary
Silencing GhBLH7-D06 enhanced cotton resistance to Verticillium wilt.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- silencing the expression of GhBLH7-D06e0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhBLH7-D06e0
- Outcome
- resistance of cotton plants to Verticillium wilte0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing the expression of GhBLH7-D06 could enhance the resistance of cotton plants to Verticillium wilt”
Claim · model-written summary
Silencing GhBLH7-D06 significantly overexpressed lignin-biosynthesis genes.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- silencing the expression of GhBLH7-D06e1
- Gene
- genes related to lignin biosynthesise0
- Outcome
- genes related to lignin biosynthesise0
- Direction
- overexpressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the significant overexpression of genes related to lignin biosynthesis and JA signaling pathway”
e1“silencing the expression of GhBLH7-D06 could enhance the resistance of cotton plants to Verticillium wilt”
t0“Cotton BEL1-Like Transcription Factor GhBLH7-D06”
Claim · model-written summary
Silencing GhBLH7-D06 significantly overexpressed JA-signaling genes.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- genes related to lignin biosynthesis and JA signaling pathwaye0
- Direction
- overexpressione0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the significant overexpression of genes related to lignin biosynthesis and JA signaling pathway”
t0“Cotton”
Claim · model-written summary
GhOFP3-D13 interacted with GhBLH7-D06.
- Taxon
- cottonGossypium hirsutume0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhOFP3-D13e0
- Outcome
- interacts with GhBLH7-D06e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an Ovate Family Protein (OFP) transcription factor GhOFP3-D13 which was also a negative regulator of cotton Verticillium wilt resistance could that interacts with GhBLH7-D06”
Claim · model-written summary
GhOFP3-D13 negatively regulated cotton Verticillium wilt resistance.
- Taxon
- cottonGossypium hirsutume0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhOFP3-D13e0
- Outcome
- cotton Verticillium wilt resistancee0
- Direction
- negativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhOFP3-D13 which was also a negative regulator of cotton Verticillium wilt resistance”
Claim · model-written summary
GhBLH7-D06 bound the GhPAL-A06 promoter and suppressed its expression.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhPAL-A06e0
- Outcome
- its expressione0
- Direction
- suppresse0
- Qualifier
- target binding to the promoter regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhBLH7-D06 could target binding to the promoter region of GhPAL-A06 to suppress its expression”
t0“Cotton”
Claim · model-written summary
GhBLH7-D06 suppression of GhPAL-A06 inhibited lignin biosynthesis.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhPAL-A06e0
- Outcome
- lignin biosynthesise0
- Direction
- inhibitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhBLH7-D06 could target binding to the promoter region of GhPAL-A06 to suppress its expression and eventually lead to the inhibition of lignin biosynthesis”
t0“Cotton”
Claim · model-written summary
The GhBLH7-D06/GhOFP3-D13 complex negatively regulated cotton resistance to Verticillium wilt through inhibited lignin biosynthesis and JA signaling.
- Taxon
- cottonGossypium hirsutume0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhBLH7-D06/GhOFP3-D13e0
- Outcome
- resistance to Verticillium wilte0
- Direction
- negatively regulatee0
- Qualifier
- inhibiting lignin biosynthesis and JA signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the GhBLH7-D06/GhOFP3-D13 complex can negatively regulate resistance to Verticillium wilt of cotton by inhibiting lignin biosynthesis and JA signaling pathway”
The same questions in other papers
loss of function · Verticillium · Gossypium hirsutum (cotton)Positive and negative results
6 papers in the same direction as this paper · 8 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →transgenic · Verticillium · Gossypium hirsutum (cotton)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.