Abstract
Candidate clones which conferred Al tolerance to yeast transformants (TFs) were obtained from a cDNA library derived from a highly Al-tolerant poaceae, Andropogon virginicus L. One such clone, AL3A-4, encoded an S-adenosyl methionine synthetase (SAMS) gene. A full-length cDNA was obtained by 5’-RACE, designated AvSAMS1, and introduced into Arabidopsis thaliana to investigate its biological functions under Al stress. Two TF plant lines both showed higher tolerance than the Col-0 ecotype (non-TF) not only for Al stress, but also for Cu, Pb, Zn and diamide stresses, suggesting the AvSAMS1 was a multiple tolerance gene. More than 40 of A. thaliana Al response-genes (Al induced genes and Al repressed genes) were selected from microarray results and then used for investigations of DNA or histone methylation status under Al stress in Col-0 and the AvSAMS1 TF line. The results indicated that Al stress caused alterations of methylation status in both DNA and histone H3 (H3K4me3 and H3K9me3) and that these alterations were different between the AvSAMS1 TF and Col-0, suggesting the differences were AvSAMS1-gene dependent. These results suggested the existence of AvSAMS1-related epigenetic gene-regulation under Al stress.
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Claims 9
Claim · model-written summary
AvSAMS1 was identified as a SAMS gene from Andropogon virginicus.
- Taxon
- Andropogon virginicus L.
Anatherum virginicume0 - Gene
- AvSAMS1e0
- Outcome
- S-adenosyl methionine synthetase (SAMS) genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a highly Al-tolerant poaceae, Andropogon virginicus L. One such clone, AL3A-4, encoded an S-adenosyl methionine synthetase (SAMS) gene. A full-length cDNA was obtained by 5’-RACE, designated AvSAMS1”
Claim · model-written summary
AvSAMS1 transformant Arabidopsis lines had higher aluminum tolerance than Col-0.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- TF plant linese1
- Treatment
- Al stresse1
- Outcome
- tolerancee1
- Direction
- highere1
- Comparator
- Col-0 ecotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“introduced into Arabidopsis thaliana”
e1“Two TF plant lines both showed higher tolerance than the Col-0 ecotype (non-TF) not only for Al stress, but also for Cu, Pb, Zn and diamide stresses”
Claim · model-written summary
AvSAMS1 transformant Arabidopsis lines had higher copper tolerance than Col-0.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- TF plant linese1
- Treatment
- Cue1
- Outcome
- tolerancee1
- Direction
- highere1
- Comparator
- Col-0 ecotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“introduced into Arabidopsis thaliana”
e1“Two TF plant lines both showed higher tolerance than the Col-0 ecotype (non-TF) not only for Al stress, but also for Cu, Pb, Zn and diamide stresses”
Claim · model-written summary
AvSAMS1 transformant Arabidopsis lines had higher lead tolerance than Col-0.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- TF plant linese1
- Treatment
- Pbe1
- Outcome
- tolerancee1
- Direction
- highere1
- Comparator
- Col-0 ecotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“introduced into Arabidopsis thaliana”
e1“Two TF plant lines both showed higher tolerance than the Col-0 ecotype (non-TF) not only for Al stress, but also for Cu, Pb, Zn and diamide stresses”
Claim · model-written summary
AvSAMS1 transformant Arabidopsis lines had higher zinc tolerance than Col-0.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- TF plant linese1
- Treatment
- Zne1
- Outcome
- tolerancee1
- Direction
- highere1
- Comparator
- Col-0 ecotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“introduced into Arabidopsis thaliana”
e1“Two TF plant lines both showed higher tolerance than the Col-0 ecotype (non-TF) not only for Al stress, but also for Cu, Pb, Zn and diamide stresses”
Claim · model-written summary
AvSAMS1 transformant Arabidopsis lines had higher diamide tolerance than Col-0.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- TF plant linese1
- Treatment
- diamide stressese1
- Outcome
- tolerancee1
- Direction
- highere1
- Comparator
- Col-0 ecotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“introduced into Arabidopsis thaliana”
e1“Two TF plant lines both showed higher tolerance than the Col-0 ecotype (non-TF) not only for Al stress, but also for Cu, Pb, Zn and diamide stresses”
Claim · model-written summary
Aluminum stress altered DNA methylation in Arabidopsis.
- Taxon
- A. thalianaArabidopsis thalianae0
- Treatment
- Al stresse1
- Outcome
- methylation status in both DNAe1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. thaliana Al response-genes”
e1“Al stress caused alterations of methylation status in both DNA and histone H3 (H3K4me3 and H3K9me3)”
Claim · model-written summary
Aluminum stress altered histone H3 methylation in Arabidopsis.
- Taxon
- A. thalianaArabidopsis thalianae0
- Treatment
- Al stresse1
- Outcome
- histone H3 (H3K4me3 and H3K9me3)e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. thaliana Al response-genes”
e1“Al stress caused alterations of methylation status in both DNA and histone H3 (H3K4me3 and H3K9me3)”
Claim · model-written summary
Aluminum induced methylation changes differed between AvSAMS1 transformants and Col-0.
- Taxon
- A. thalianaArabidopsis thalianae0
- Material
- AvSAMS1 TFe1
- Gene
- AvSAMS1e1
- Outcome
- these alterations were differente1
- Comparator
- Col-0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. thaliana Al response-genes”
e1“these alterations were different between the AvSAMS1 TF and Col-0, suggesting the differences were AvSAMS1-gene dependent.”