Abstract
α-SNAP [soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein] and NSF proteins are conserved across eukaryotes and sustain cellular vesicle trafficking by mediating disassembly and reuse of SNARE protein complexes, which facilitate fusion of vesicles to target membranes. However, certain haplotypes of the Rhg1 (resistance to Heterodera glycines 1) locus of soybean possess multiple repeat copies of an α-SNAP gene (Glyma.18G022500) that encodes atypical amino acids at a highly conserved functional site. These Rhg1 loci mediate resistance to soybean cyst nematode (SCN; H. glycines), the most economically damaging pathogen of soybeans worldwide. Rhg1 is widely used in agriculture, but the mechanisms of Rhg1 disease resistance have remained unclear. In the present study, we found that the resistance-type Rhg1 α-SNAP is defective in interaction with NSF. Elevated in planta expression of resistance-type Rhg1 α-SNAPs depleted the abundance of SNARE-recycling 20S complexes, disrupted vesicle trafficking, induced elevated abundance of NSF, and caused cytotoxicity. Soybean, due to ancient genome duplication events, carries other loci that encode canonical (wild-type) α-SNAPs. Expression of these α-SNAPs counteracted the cytotoxicity of resistance-type Rhg1 α-SNAPs. For successful growth and reproduction, SCN dramatically reprograms a set of plant root cells and must sustain this sedentary feeding site for 2-4 weeks. Immunoblots and electron microscopy immunolocalization revealed that resistance-type α-SNAPs specifically hyperaccumulate relative to wild-type α-SNAPs at the nematode feeding site, promoting the demise of this biotrophic interface. The paradigm of disease resistance through a dysfunctional variant of an essential gene may be applicable to other plant-pathogen interactions.
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Claims 8
Claim · model-written summary
Resistance-type Rhg1 α-SNAP interacted defectively with NSF.
- Taxon
- soybean
Glycine maxt0 - Gene
- Rhg1 α-SNAPe0
- Outcome
- interaction with NSFe0
- Direction
- defectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance-type Rhg1 α-SNAP is defective in interaction with NSF”
t0“soybean”
Claim · model-written summary
Elevated resistance-type Rhg1 α-SNAP expression depleted SNARE-recycling 20S complexes.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Elevated in planta expressione0
- Gene
- Rhg1 α-SNAPse0
- Outcome
- abundance of SNARE-recycling 20S complexese0
- Direction
- depletede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated in planta expression of resistance-type Rhg1 α-SNAPs depleted the abundance of SNARE-recycling 20S complexes”
t0“soybean”
Claim · model-written summary
Elevated resistance-type Rhg1 α-SNAP expression disrupted vesicle trafficking.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Elevated in planta expressione0
- Gene
- Rhg1 α-SNAPse0
- Outcome
- vesicle traffickinge0
- Direction
- disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated in planta expression of resistance-type Rhg1 α-SNAPs depleted the abundance of SNARE-recycling 20S complexes, disrupted vesicle trafficking”
t0“soybean”
Claim · model-written summary
Elevated resistance-type Rhg1 α-SNAP expression increased NSF abundance.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Elevated in planta expression of resistance-type Rhg1 α-SNAPse0
- Gene
- NSFe0
- Outcome
- abundance of NSFe0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated in planta expression of resistance-type Rhg1 α-SNAPs depleted the abundance of SNARE-recycling 20S complexes, disrupted vesicle trafficking, induced elevated abundance of NSF”
t0“soybean”
Claim · model-written summary
Elevated resistance-type Rhg1 α-SNAP expression caused cytotoxicity.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Elevated in planta expressione0
- Gene
- Rhg1 α-SNAPse0
- Outcome
- cytotoxicitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated in planta expression of resistance-type Rhg1 α-SNAPs depleted the abundance of SNARE-recycling 20S complexes, disrupted vesicle trafficking, induced elevated abundance of NSF, and caused cytotoxicity”
t0“soybean”
Claim · model-written summary
Expression of canonical α-SNAPs counteracted cytotoxicity caused by resistance-type α-SNAPs.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Expression of these α-SNAPse0
- Gene
- α-SNAPse0
- Outcome
- cytotoxicitye0
- Direction
- counteractede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of these α-SNAPs counteracted the cytotoxicity of resistance-type Rhg1 α-SNAPs”
t0“soybean”
Claim · model-written summary
Resistance-type α-SNAPs hyperaccumulated at nematode feeding sites relative to wild-type α-SNAPs.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- nematode feeding sitee0
- Gene
- resistance-type α-SNAPse0
- Outcome
- α-SNAPse0
- Direction
- hyperaccumulatee0
- Comparator
- wild-type α-SNAPse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance-type α-SNAPs specifically hyperaccumulate relative to wild-type α-SNAPs at the nematode feeding site”
t0“soybean”
Claim · model-written summary
Hyperaccumulation of resistance-type α-SNAPs promoted demise of the feeding interface.
- Taxon
- soybeanGlycine maxt0
- Gene
- resistance-type α-SNAPse0
- Outcome
- demise of this biotrophic interfacee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance-type α-SNAPs specifically hyperaccumulate relative to wild-type α-SNAPs at the nematode feeding site, promoting the demise of this biotrophic interface”
t0“soybean”
The same questions in other papers
transgenic · cytology · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.