Abstract
In Arabidopsis, recognition of the AvrPphB effector protease from Pseudomonas syringae is mediated by the disease resistance (R) protein RPS5, which is activated by AvrPphB-induced cleavage of the Arabidopsis protein kinase PBS1. The recognition specificity of RPS5 can be altered by substituting the AvrPphB cleavage site within PBS1 with cleavage sequences for other proteases, including proteases from viruses. AvrPphB also activates defense responses in soybean (Glycine max), suggesting that soybean may contain an R protein analogous to RPS5. It was unknown, however, whether this response is mediated by cleavage of a soybean PBS1-like protein. Here, we show that soybean contains three PBS1 orthologs and that their products are cleaved by AvrPphB. Further, transient expression of soybean PBS1 derivatives containing a five-alanine insertion at their AvrPphB cleavage sites activated cell death in soybean protoplasts, demonstrating that soybean likely contains an AvrPphB-specific resistance protein that is activated by a conformational change in soybean PBS1 proteins. Significantly, we show that a soybean PBS1 decoy protein modified to contain a cleavage site for the soybean mosaic virus (SMV) NIa protease triggers cell death in soybean protoplasts when cleaved by this protease, indicating that the PBS1 decoy approach will work in soybean, using endogenous PBS1 genes. Lastly, we show that activation of the AvrPphB-dependent cell death response effectively inhibits systemic spread of SMV in soybean. These data also indicate that decoy engineering may be feasible in other crop plant species that recognize AvrPphB protease activity.
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Claims 6
Claim · model-written summary
Soybean has three PBS1 orthologs whose products AvrPphB cleaves.
- Taxon
- soybean
Glycine maxe0 - Gene
- PBS1 orthologse0
- Outcome
- three PBS1 orthologs and that their products are cleaved by AvrPphBe0
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean contains three PBS1 orthologs and that their products are cleaved by AvrPphB.”
Claim · model-written summary
Five-alanine insertion in soybean PBS1 derivatives activated cell death in soybean protoplasts.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- protoplastse0
- Treatment
- transient expression of soybean PBS1 derivatives containing a five-alanine insertione0
- Gene
- soybean PBS1e0
- Outcome
- cell death in soybean protoplastse0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of soybean PBS1 derivatives containing a five-alanine insertion at their AvrPphB cleavage sites activated cell death in soybean protoplasts”
Claim · model-written summary
The PBS1 insertion result suggests soybean has an AvrPphB-specific resistance protein activated by PBS1 conformational change.
- Taxon
- soybeanGlycine maxe0
- Gene
- soybean PBS1 proteinse0
- Outcome
- AvrPphB-specific resistance proteine0
- Qualifier
- activated by a conformational changee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean likely contains an AvrPphB-specific resistance protein that is activated by a conformational change in soybean PBS1 proteins.”
Claim · model-written summary
Engineered soybean PBS1 decoy cleavage by SMV NIa protease triggered cell death in soybean protoplasts.
- Taxon
- soybeanGlycine maxe0
- Treatment
- modified to contain a cleavage site for the soybean mosaic virus (SMV) NIa proteasee0
- Interactor
- soybean mosaic virus (SMV)
Soybean mosaic viruse0pathogen - Gene
- soybean PBS1 decoy proteine0
- Outcome
- cell death in soybean protoplastse0
- Direction
- triggerse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a soybean PBS1 decoy protein modified to contain a cleavage site for the soybean mosaic virus (SMV) NIa protease triggers cell death in soybean protoplasts when cleaved by this protease”
Claim · model-written summary
Activation of AvrPphB-dependent cell death inhibited systemic spread of SMV in soybean.
- Taxon
- soybeanGlycine maxe0
- Treatment
- activation of the AvrPphB-dependent cell death responsee0
- Interactor
- soybean mosaic virus (SMV)Soybean mosaic viruse1pathogen
- Outcome
- systemic spread of SMVe0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activation of the AvrPphB-dependent cell death response effectively inhibits systemic spread of SMV in soybean.”
e1“soybean mosaic virus (SMV)”
Claim · model-written summary
The authors infer that decoy engineering may be feasible in other crop species recognizing AvrPphB activity.
Verbatim
e0“decoy engineering may be feasible in other crop plant species that recognize AvrPphB protease activity.”
The same questions in other papers
transgenic · cytology · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.