Paper · 1995 · HortScience

Development of a Nutrient Medium for the Growth of Date Palm (Phoenix dactylifera L.) Roots by tTissue Culture

Yousef I. Dlaigen; A. E. Said; M.A. El-Hamady

10
claims
21
verbatim quotes
0
citations
2
research areas
✓
Open access · public-domain

Abstract

Several experiments were conducted in this investigation with the objective of determining the chemical components and the physical state of an optimal medium for the growth and elongation of excised date palm, cv Sukkari, roots. The chemical tests carried out included: Comparison of (MS)-salts with “White's”-salts mixture and different concentrations of (MS)-salts and its chelated iron; sugars; Modified White's Organics; inositol; adenine sulfate; growth regulators; and some antioxidants. The physical tests, on the other hand, included comparison of the growth and elongation of cultured roots in a liquid or on solidified nutrient media. The effects of various pH values were also tested. Roots were cultured in basal nutrient media composed of: (MS)-salts mixture, and (in mg·liter –1 ): NaH 2 PO 4 ·H 2 O, 170; sucrose, 30,000; inositol, 200; Modified White's Organics; adenine sulfate, 120; activated charcoal, 1500; (2,4-D), 1; kinetin, 2. pH was adjusted at 5.7 ± 01. (MS)-salts mixture was found to be superior to “White's”-salts. No significant difference was observed between (1/2MS) and full-strength (MS)-salts. However, twice the concentration was found to be inhibitory. The normal concentration of (MS)-Fe was found to be optimum for root growth and elongation. The optimal concentration most suitable for the growth and elongation of excised date palm roots has been determined for each of: sugars; Modified White's Organics; inositol; and adenine sulfate. The only growth regulator that needs to be added to the nutrient medium is 2,4-D at 0.1–1.0 mg·liter –1 . The study showed the importance of the inclusion of activated charcoal to the nutrient medium. The growth and elongation of roots were both stimulated at all concentrations tested. (PVP), on the other hand, was inhibitory at all concentrations tested. Shaken liquid media was recommended for better root growth and elongation at pH 7.0–8.0. Incidentally, the medium developed was found to support the growth and elongation of roots excised from two other cultivars, namely `Khudri' and `Khaias'.

Abstract from the paper’s OpenAlex record; open copy licensed public-domain. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS salts were superior to White's salts for excised date palm root growth.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
(MS)-salts mixturee1
Outcome
superiore1
Direction
superiore1
Comparator
White'se1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e1

“(MS)-salts mixture was found to be superior to “White's”-salts”

✓ located verbatim in the source · abstract, characters 981–1043 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Half-strength and full-strength MS salts gave no significant difference.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
(1/2MS)e2
Outcome
differencee2
Direction
No significant differencee2
Comparator
full-strength (MS)-saltse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e2

“No significant difference was observed between (1/2MS) and full-strength (MS)-salts”

✓ located verbatim in the source · abstract, characters 1045–1128 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Twice the concentration of the MS salts was inhibitory for excised date palm roots.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
twice the concentratione3
Outcome
inhibitorye3
Direction
inhibitorye3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e3

“twice the concentration was found to be inhibitory”

✓ located verbatim in the source · abstract, characters 1139–1189 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The normal concentration of MS-Fe was optimum for root growth and elongation of excised date palm roots.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
normal concentration of (MS)-Fee4
Outcome
root growth and elongatione4
Direction
optimume4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e4

“The normal concentration of (MS)-Fe was found to be optimum for root growth and elongation”

✓ located verbatim in the source · abstract, characters 1191–1281 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

2,4-D at 0.1–1.0 mg/l is recommended as the only growth regulator to add to the medium for date palm roots.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
2,4-De5
Outcome
only growth regulatore5
Value
0.1–1.0 mg·liter –1e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e5

“The only growth regulator that needs to be added to the nutrient medium is 2,4-D at 0.1–1.0 mg·liter –1”

✓ located verbatim in the source · abstract, characters 1479–1582 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Activated charcoal stimulated growth and elongation of excised date palm roots at all concentrations tested.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
activated charcoale6
Outcome
growth and elongation of rootse7
Direction
stimulatede7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e6

“The study showed the importance of the inclusion of activated charcoal to the nutrient medium”

✓ located verbatim in the source · abstract, characters 1585–1678 · show in text ↑
e7

“The growth and elongation of roots were both stimulated at all concentrations tested”

✓ located verbatim in the source · abstract, characters 1680–1764 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

PVP was inhibitory at all concentrations tested for excised date palm roots.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
PVPe1
Outcome
inhibitorye1
Direction
inhibitorye1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e1

“(PVP), on the other hand, was inhibitory at all concentrations tested”

✓ located verbatim in the source · abstract, characters 1766–1835 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Shaken liquid media is recommended for better root growth and elongation at pH 7.0–8.0.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Treatment
Shaken liquid mediae1
Outcome
better root growth and elongatione1
Qualifier
at pH 7.0–8.0e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e1

“Shaken liquid media was recommended for better root growth and elongation at pH 7.0–8.0”

✓ located verbatim in the source · abstract, characters 1837–1924 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The developed medium supported growth and elongation of roots excised from two other date palm cultivars.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Material
`Khudri' and `Khaias'e1
Outcome
growth and elongation of rootse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e1

“the medium developed was found to support the growth and elongation of roots excised from two other cultivars, namely `Khudri' and `Khaias'”

✓ located verbatim in the source · abstract, characters 1940–2079 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Optimal concentrations were determined for sugars, Modified White's Organics, inositol and adenine sulfate.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Outcome
optimal concentratione1
Qualifier
sugars; Modified White's Organics; inositol; and adenine sulfatee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date Palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 51–85 · show in text ↑
e1

“The optimal concentration most suitable for the growth and elongation of excised date palm roots has been determined for each of: sugars; Modified White's Organics; inositol; and adenine sulfate”

✓ located verbatim in the source · abstract, characters 1283–1477 · show in text ↑

charcoal · growth · Phoenix dactylifera (date palm)Results differ

16 papers: 15 positive, 1 negative or no effect

14 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.