Abstract
BACKGROUND AND OBJECTIVE: Somatic embryogenesis techniques are used for cloning a wide range of varieties of date palms around the world. The aim of the present study was to develop an efficient method with the lowest cost and the greatest potential to obtain in vitro plantlets of date palm cv. Medjool. Also, produce embryogenic callus and somatic embryos without using 2,4-dichlorophenoxyacetic acid (2,4-D). METHODOLOGY: In this study, produced plantlets through somatic embryogenesis were used in vitro roots as explant cultured on Murashige and Skoog (MS) media containing three level of Silver Nitrate (AgNO3) (0, 3 and 6 mg L-1) plus two level of 6-benzylaminopurine (BAP) (0 and 2 mg L-1) plus 0.1 mg L-1 1-naphthylacetic acid (NAA) for callus induction. After 12 weeks of culture, callus induction and after 16 weeks, production of embryogenic callus and embryos were occurred from root explants. RESULTS: According to the results, medium containing 2 mg L-1 BAP and 3 mg L-1 silver nitrate+0.1 mg L-1 NAA showed the highest amount of embryogenic callus fresh weight (1.38 g). This treatment also cause the highest number and length of embryos by production of 90.04 embryogenic callus with length of 11.18 mm. On the other hand, shoots were appeared from germinated embryos and white roots began to appear within 8 weeks. Medium contains 3 mg L-1 BAP and 0.1 mg L-1 NAA with average of 12.27 cm shoot length and 15.48 cm root length was the best. Control treatment had the lowest average shoot (3.71 cm) and root (5.03 cm) length. CONCLUSION: This study showed that certain concentration of silver nitrate and BAP has stimulating effect on growth of produced embryonic callus from root segments of Medjool cultivar of date palm.
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Claims 6
Claim · model-written summary
The medium with 2 mg/L BAP and 3 mg/L silver nitrate plus 0.1 mg/L NAA gave the highest embryogenic callus fresh weight (1.38 g) in Medjool date palm root explants.
- Taxon
- Phoenix dactylifera
Phoenix dactyliferat0 - Material
- Medjoolt0
- Organ / stage
- root explantse0
- Treatment
- 2 mg L-1 BAP and 3 mg L-1 silver nitrate+0.1 mg L-1 NAAe1
- Outcome
- embryogenic callus fresh weighte1
- Direction
- higheste1
- Value
- 1.38 ge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effect of AgNO3 and BAP on Root as a Novel Explant in Date Palm (Phoenix dactylifera cv. Medjool) Somatic Embryogenesis”
e0“production of embryogenic callus and embryos were occurred from root explants”
e1“medium containing 2 mg L-1 BAP and 3 mg L-1 silver nitrate+0.1 mg L-1 NAA showed the highest amount of embryogenic callus fresh weight (1.38 g)”
Claim · model-written summary
The same medium gave the highest number and length of embryos in Medjool date palm.
- Taxon
- Phoenix dactyliferaPhoenix dactyliferat0
- Material
- Medjoolt0
- Treatment
- 2 mg L-1 BAP and 3 mg L-1 silver nitrate+0.1 mg L-1 NAAe1
- Outcome
- number and length of embryose2
- Direction
- higheste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effect of AgNO3 and BAP on Root as a Novel Explant in Date Palm (Phoenix dactylifera cv. Medjool) Somatic Embryogenesis”
e1“medium containing 2 mg L-1 BAP and 3 mg L-1 silver nitrate+0.1 mg L-1 NAA showed the highest amount of embryogenic callus fresh weight (1.38 g)”
e2“This treatment also cause the highest number and length of embryos”
Claim · model-written summary
Embryogenic callus with a length of 11.18 mm was produced under the same medium in date palm.
- Taxon
- Phoenix dactyliferaPhoenix dactyliferat0
- Treatment
- 2 mg L-1 BAP and 3 mg L-1 silver nitrate+0.1 mg L-1 NAAe1
- Outcome
- embryogenic callus with lengthe3
- Value
- 11.18 mme3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effect of AgNO3 and BAP on Root as a Novel Explant in Date Palm (Phoenix dactylifera cv. Medjool) Somatic Embryogenesis”
e1“medium containing 2 mg L-1 BAP and 3 mg L-1 silver nitrate+0.1 mg L-1 NAA showed the highest amount of embryogenic callus fresh weight (1.38 g)”
e3“production of 90.04 embryogenic callus with length of 11.18 mm”
Claim · model-written summary
The medium with 3 mg/L BAP and 0.1 mg/L NAA gave the best shoot length (12.27 cm) in date palm plantlets, against a control of 3.71 cm.
- Taxon
- Phoenix dactyliferaPhoenix dactyliferat0
- Material
- Medjoolt0
- Treatment
- 3 mg L-1 BAP and 0.1 mg L-1 NAAe4
- Outcome
- shoot lengthe4
- Direction
- beste4
- Value
- 12.27 cme4
- Qualifier
- 3.71 cme5
- Comparator
- Control treatmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effect of AgNO3 and BAP on Root as a Novel Explant in Date Palm (Phoenix dactylifera cv. Medjool) Somatic Embryogenesis”
e4“Medium contains 3 mg L-1 BAP and 0.1 mg L-1 NAA with average of 12.27 cm shoot length and 15.48 cm root length was the best”
e5“Control treatment had the lowest average shoot (3.71 cm) and root (5.03 cm) length”
Claim · model-written summary
The medium with 3 mg/L BAP and 0.1 mg/L NAA gave the best root length (15.48 cm) in date palm plantlets, against a control of 5.03 cm.
- Taxon
- Phoenix dactyliferaPhoenix dactyliferat0
- Material
- Medjoolt0
- Treatment
- 3 mg L-1 BAP and 0.1 mg L-1 NAAe4
- Outcome
- root lengthe4
- Direction
- beste4
- Value
- 15.48 cme4
- Qualifier
- 5.03 cme5
- Comparator
- Control treatmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effect of AgNO3 and BAP on Root as a Novel Explant in Date Palm (Phoenix dactylifera cv. Medjool) Somatic Embryogenesis”
e4“Medium contains 3 mg L-1 BAP and 0.1 mg L-1 NAA with average of 12.27 cm shoot length and 15.48 cm root length was the best”
e5“Control treatment had the lowest average shoot (3.71 cm) and root (5.03 cm) length”
Claim · model-written summary
Shoots and white roots appeared from germinated embryos in date palm within 8 weeks.
- Taxon
- Phoenix dactyliferaPhoenix dactyliferat0
- Outcome
- shoots were appeared from germinated embryos and white roots began to appeare6
- Qualifier
- within 8 weekse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effect of AgNO3 and BAP on Root as a Novel Explant in Date Palm (Phoenix dactylifera cv. Medjool) Somatic Embryogenesis”
e6“shoots were appeared from germinated embryos and white roots began to appear within 8 weeks”
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