Abstract
A successful protocol for the in vitro regeneration of sugar beet was established. Leaf and shoot base explants excised from in vitro germinated seedlings of sugar beet (Beta vulgaris) were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of auxin and cytokinin. Shoot base explants showed higher percentage of direct organogenesis (93%) when cultured on MS medium supplemented with 0.5 mg/l of 6-benzyladenine (BA) and 0.5 mg/l of 1-naphtaleneacetic acid (NAA) than leaf explants cultured on MS medium containing 0.5 mg/l of 6-furfurylaminopurine (kin) and 0.5 mg/l of 2,4-dicholorophenoxyacetic acid (2,4-D) , which showed more efficient indirect regeneration (80%). Although maximum shoot proliferation was observed with MS medium containing 1 mg/l BA and 50 mg/l adenine sulfate (AS), shoot length reached 4 cm with MS medium containing 1 mg/l kin and 50 mg/l AS. Regenerated shoots rooted on indole-3-butyric acid (IBA) at 2 mg/l that seemed more effective than indole-3-acetic acid (IAA) and NAA in root formation. Protein pattern (SDS-PAGE) evidenced no variations between sugar beet cultures proliferated directly and indirectly. RAPD analysis was carried out to identify the differences amongst in vitro regenerated plantlets. PCR analysis showed slight differences between in vitro and in vivo sugar beet plantlets. Acclimatization was achieved by transferring the obtained plantlets to a mixture of peat-moss and vermiculite (1:1) under high humidity conditions.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Shoot base explants of sugar beet showed higher direct organogenesis (93%) on MS medium with 0.5 mg/l BA and 0.5 mg/l NAA than leaf explants.
- Taxon
- Sugar Beet
Beta vulgaris subsp. vulgarist0 - Organ / stage
- Shoot base explantse0
- Treatment
- MS medium supplemented with 0.5 mg/l of 6-benzyladenine (BA) and 0.5 mg/l of 1-naphtaleneacetic acid (NAA)e0
- Outcome
- percentage of direct organogenesise0
- Direction
- highere0
- Value
- 93%e0
- Comparator
- leaf explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sugar Beet”
e0“Shoot base explants showed higher percentage of direct organogenesis (93%) when cultured on MS medium supplemented with 0.5 mg/l of 6-benzyladenine (BA) and 0.5 mg/l of 1-naphtaleneacetic acid (NAA) than leaf explants”
Claim · model-written summary
Leaf explants on MS medium with 0.5 mg/l kin and 0.5 mg/l 2,4-D showed more efficient indirect regeneration (80%).
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Organ / stage
- leaf explantse0
- Treatment
- MS medium containing 0.5 mg/l of 6-furfurylaminopurine (kin) and 0.5 mg/l of 2,4-dicholorophenoxyacetic acid (2,4-D)e0
- Outcome
- indirect regeneratione0
- Direction
- more efficiente0
- Value
- 80%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sugar Beet”
e0“leaf explants cultured on MS medium containing 0.5 mg/l of 6-furfurylaminopurine (kin) and 0.5 mg/l of 2,4-dicholorophenoxyacetic acid (2,4-D) , which showed more efficient indirect regeneration (80%)”
Claim · model-written summary
Maximum shoot proliferation occurred on MS medium containing 1 mg/l BA and 50 mg/l adenine sulfate.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- MS medium containing 1 mg/l BA and 50 mg/l adenine sulfate (AS)e0
- Outcome
- shoot proliferatione0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sugar Beet”
e0“maximum shoot proliferation was observed with MS medium containing 1 mg/l BA and 50 mg/l adenine sulfate (AS)”
Claim · model-written summary
Shoot length reached 4 cm on MS medium containing 1 mg/l kin and 50 mg/l AS.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- MS medium containing 1 mg/l kin and 50 mg/l ASe0
- Outcome
- shoot lengthe0
- Direction
- increaseinferrede0
- Value
- 4 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sugar Beet”
e0“shoot length reached 4 cm with MS medium containing 1 mg/l kin and 50 mg/l AS”
Claim · model-written summary
Regenerated shoots rooted on IBA at 2 mg/l, which seemed more effective than IAA and NAA in root formation.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- indole-3-butyric acid (IBA) at 2 mg/le0
- Outcome
- root formatione0
- Direction
- more effectivee0
- Comparator
- indole-3-acetic acid (IAA) and NAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sugar Beet”
e0“Regenerated shoots rooted on indole-3-butyric acid (IBA) at 2 mg/l that seemed more effective than indole-3-acetic acid (IAA) and NAA in root formation”
Claim · model-written summary
SDS-PAGE protein patterns showed no variations between sugar beet cultures proliferated directly and indirectly.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Outcome
- Protein pattern (SDS-PAGE)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein pattern (SDS-PAGE) evidenced no variations between sugar beet cultures proliferated directly and indirectly”
Claim · model-written summary
PCR analysis showed slight differences between in vitro and in vivo sugar beet plantlets.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Outcome
- slight differences between in vitro and in vivo sugar beet plantletse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR analysis showed slight differences between in vitro and in vivo sugar beet plantlets”
Claim · model-written summary
Plantlets were acclimatized by transfer to a peat-moss and vermiculite (1:1) mixture under high humidity.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Outcome
- Acclimatization was achievede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sugar Beet”
e0“Acclimatization was achieved by transferring the obtained plantlets to a mixture of peat-moss and vermiculite (1:1) under high humidity conditions”
The same questions in other papers
2,4-D · growth · Beta vulgaris (beet)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
sulfate · growth · Beta vulgaris (beet)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
adenine · growth · Beta vulgaris (beet)Results agree
1 paper in the same direction as this paper
- positive
indole-3-butyric acid · growth · Beta vulgaris (beet)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Beta vulgaris (beet)Results agree
9 papers in the same direction as this paper
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →in vitro · growth · Beta vulgaris (beet)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.