Abstract
For callus production, hypocotyl, cotyledon, petiole and leaf explants from seedlings of different sugar beet ( Beta vulgaris L.) breeding lines were cultured on MS medium containing BAP or KIN in combination with NAA or 2,4-D at 0.0, 0.5 or 1.0 mg/l. The use of both auxins in combination with 0.5 mg/l BAP or 0.5 mg/l KIN resulted in greater amounts of callus from all types of explants. However, hypocotyl and cotyledon explants produced significantly more callus than petiole and leaf explants when the means of all lines were taken into account. Two types of callus were usually obtained, white and friable callus with large cells (Type I) and green and compact callus with smaller cells (Type II). For shoot induction, Type I callus was transferred to MS medium containing 2.5 mg/l TDZ or 1.0 mg/l BAP in combination with 0.3 mg/l IAA, together with or without a cold pre-treatment at 4¡C for two weeks. More shoots developed from the pre-treated callus and elongated shoots could be readily rooted on MS medium containing 3.0 mg/l IBA.
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Claims 6
Claim · model-written summary
Both auxins with 0.5 mg/l BAP produced greater callus from all explant types.
- Taxon
- sugar beet
Beta vulgaris subsp. vulgarist0 - Treatment
- both auxins in combination with 0.5 mg/l BAPe0
- Outcome
- amounts of calluse0
- Direction
- greatere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of both auxins in combination with 0.5 mg/l BAP or 0.5 mg/l KIN resulted in greater amounts of callus from all types of explants.”
t0“Sugar Beet ( Beta vulgarisL.)”
Claim · model-written summary
Both auxins with 0.5 mg/l KIN produced greater callus from all explant types.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Treatment
- 0.5 mg/l KINe0
- Outcome
- amounts of calluse0
- Direction
- greatere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of both auxins in combination with 0.5 mg/l BAP or 0.5 mg/l KIN resulted in greater amounts of callus from all types of explants.”
t0“Sugar Beet ( Beta vulgarisL.)”
Claim · model-written summary
Hypocotyl and cotyledon explants yielded more callus than petiole and leaf explants.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Organ / stage
- hypocotyl and cotyledon explantse0
- Outcome
- calluse0
- Direction
- moree0
- Comparator
- petiole and leaf explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hypocotyl and cotyledon explants produced significantly more callus than petiole and leaf explants when the means of all lines were taken into account”
t0“Sugar Beet ( Beta vulgarisL.)”
Claim · model-written summary
Two callus types were observed: white friable callus with large cells and green compact callus with smaller cells.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Outcome
- white and friable callus with large cells (Type I) and green and compact callus with smaller cells (Type II)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two types of callus were usually obtained, white and friable callus with large cells (Type I) and green and compact callus with smaller cells (Type II).”
t0“Sugar Beet ( Beta vulgarisL.)”
Claim · model-written summary
Cold pretreatment produced more shoots from callus.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Treatment
- pre-treated calluse0
- Outcome
- shootse0
- Direction
- Moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More shoots developed from the pre-treated callus”
t0“Sugar Beet ( Beta vulgarisL.)”
Claim · model-written summary
Elongated shoots rooted readily on MS medium with 3.0 mg/l IBA.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Treatment
- MS medium containing 3.0 mg/l IBAe0
- Outcome
- elongated shoots could be readily rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elongated shoots could be readily rooted on MS medium containing 3.0 mg/l IBA”
t0“Sugar Beet ( Beta vulgarisL.)”
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