Paper · 1998 · HortScience

Microscopic Examination of Chilling Injury Symptoms in Ethanol-treated Cucumber Seedling Roots

Windy A. Boyd; Paul H. Jennings

7
claims
14
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Cucumber seedlings were germinated for 24 h at 25 °C and half were immersed in a 500 mM ethanol solution for 2 h. After rinsing, seedlings were chilled for 96 h at 2 °C. Control and ethanol-treated samples were taken for light and electron microscopy immediately before and after chilling, and after re-warming for 24 and 72 h. Preliminary experiments revealed visual chilling symptoms such as pinching of the root in a region just above the root tip. This region was excised under a microscope, fixed, and mounted for microscopic observations. The cortical cells of ethanol-treated seedlings before chilling appeared to be irregular in shape with irregular edges, and some epidermal damage was evident. Chilling caused much more epidermal damage in the control seedlings when compared to the ethanol-treated seedlings. After chilling, cortical cells in the control seedlings were observed to be irregularly shaped while those treated with ethanol had round cells. Upon re-warming, control seedlings exhibited increasing epidermal damage with broken cell walls, while ethanol-treated seedlings exhibited more differentiation in the stele.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Chilling caused root pinching just above the tip.

Taxon
CucumberCucumis sativust0
Organ / stage
roote0
Outcome
pinching of the roote0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“visual chilling symptoms such as pinching of the root in a region just above the root tip”

✓ located verbatim in the source · abstract, characters 361–450 · show in text ↑
t0

“Cucumber Seedling Roots”

✓ located verbatim in the source · title, characters 71–94 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Before chilling, ethanol-treated cortical cells had irregular shape and edges.

Taxon
CucumberCucumis sativust0
Organ / stage
cortical cellse0
Treatment
ethanol-treatede0
Outcome
irregular in shape with irregular edgese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The cortical cells of ethanol-treated seedlings before chilling appeared to be irregular in shape with irregular edges”

✓ located verbatim in the source · abstract, characters 545–663 · show in text ↑
t0

“Cucumber Seedling Roots”

✓ located verbatim in the source · title, characters 71–94 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Before chilling, ethanol-treated seedlings showed some epidermal damage.

Taxon
CucumberCucumis sativust0
Outcome
epidermal damagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“some epidermal damage was evident”

✓ located verbatim in the source · abstract, characters 669–702 · show in text ↑
t0

“Cucumber Seedling Roots”

✓ located verbatim in the source · title, characters 71–94 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chilling produced more epidermal damage in controls than ethanol-treated seedlings.

Taxon
CucumberCucumis sativust0
Treatment
Chillinge0
Outcome
epidermal damagee0
Direction
much moree0
Comparator
ethanol-treated seedlingse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Chilling caused much more epidermal damage in the control seedlings when compared to the ethanol-treated seedlings”

✓ located verbatim in the source · abstract, characters 704–818 · show in text ↑
t0

“Cucumber Seedling Roots”

✓ located verbatim in the source · title, characters 71–94 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

After chilling, control cortical cells were irregular while ethanol-treated cells were round.

Taxon
CucumberCucumis sativust0
Organ / stage
cortical cellse0
Treatment
ethanole0
Outcome
round cellse0
Comparator
control seedlingse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“After chilling, cortical cells in the control seedlings were observed to be irregularly shaped while those treated with ethanol had round cells”

✓ located verbatim in the source · abstract, characters 820–963 · show in text ↑
t0

“Cucumber Seedling Roots”

✓ located verbatim in the source · title, characters 71–94 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Upon rewarming, controls had increasing epidermal damage with broken cell walls.

Taxon
CucumberCucumis sativust0
Outcome
epidermal damage with broken cell wallse0
Direction
increasinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Upon re-warming, control seedlings exhibited increasing epidermal damage with broken cell walls”

✓ located verbatim in the source · abstract, characters 965–1060 · show in text ↑
t0

“Cucumber Seedling Roots”

✓ located verbatim in the source · title, characters 71–94 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

After rewarming, ethanol-treated seedlings had more stele differentiation.

Taxon
CucumberCucumis sativust0
Organ / stage
stelee0
Treatment
ethanol-treatede0
Outcome
differentiatione0
Direction
moree0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“ethanol-treated seedlings exhibited more differentiation in the stele”

✓ located verbatim in the source · abstract, characters 1068–1137 · show in text ↑
t0

“Cucumber Seedling Roots”

✓ located verbatim in the source · title, characters 71–94 · show in text ↑

cold · pest · Cucumis sativus (cucumber)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.