Abstract
The present work reports in vitro studies carried out with two Portuguese cultivars of fig-tree: Berbera (a dried fig) and Lampa (a fresh fig). Procedures were developed to overcome difficulties associated with the establishment of adult material due to contaminants and exudation of phenolic compounds. An improved growth and development of the apical shoot-tips was obtained on Muriithi medium supplemented with 0.05 % PVP. The highest multiplication rate, 5.3 shoots per culture, every three weeks, was achieved on a similar basal medium supplemented with 2.2 mu M BA and devoid of NAA. The best rooting response (96.9 %) was observed on the medium containing 2.5 mu M IBA. Plantlets were successfully acclimatized and grown for three months in the greenhouse, and then field established for orchard production. Micropropagated plants produced fruits two years after field-establishment. These results are a promising step in the direction of in vitro cloning of valuable genotypes directly from field-grown plants and the conservation of plant genetic resources.
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Claims 5
Claim · model-written summary
Muriithi medium with 0.05 % PVP improved apical shoot-tip growth and development.
- Taxon
- FICUS CARICA L.t0
- Organ / stage
- apical shoot-tipse0
- Treatment
- Muriithi medium supplemented with 0.05 % PVPe0
- Outcome
- growth and development of the apical shoot-tipse0
- Direction
- improvede0
Not reportedComparator
Verbatim
t0“FICUS CARICA L.”
e0“An improved growth and development of the apical shoot-tips was obtained on Muriithi medium supplemented with 0.05 % PVP.”
Claim · model-written summary
Medium with 2.2 mu M BA and no NAA achieved the highest multiplication rate of 5.3 shoots per culture every three weeks.
- Taxon
- FICUS CARICA L.t0
- Treatment
- similar basal medium supplemented with 2.2 mu M BA and devoid of NAAe0
- Outcome
- multiplication ratee0
- Direction
- higheste0
- Value
- 5.3 shoots per culturee0
- Qualifier
- every three weekse0
Not reportedComparator
Verbatim
t0“FICUS CARICA L.”
e0“The highest multiplication rate, 5.3 shoots per culture, every three weeks, was achieved on a similar basal medium supplemented with 2.2 mu M BA and devoid of NAA.”
Claim · model-written summary
Medium with 2.5 mu M IBA produced the best rooting response of 96.9 %.
- Taxon
- FICUS CARICA L.t0
- Treatment
- medium containing 2.5 mu M IBAe0
- Outcome
- rooting responsee0
- Direction
- beste0
- Value
- 96.9 %e0
Not reportedComparator
Verbatim
t0“FICUS CARICA L.”
e0“The best rooting response (96.9 %) was observed on the medium containing 2.5 mu M IBA.”
Claim · model-written summary
Micropropagated plantlets were acclimatized, grown in a greenhouse and established in a field orchard.
Verbatim
t0“FICUS CARICA L.”
e0“Plantlets were successfully acclimatized and grown for three months in the greenhouse, and then field established for orchard production.”
Claim · model-written summary
Micropropagated plants fruited two years after field establishment.
Verbatim
t0“FICUS CARICA L.”
e0“Micropropagated plants produced fruits two years after field-establishment.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.