Paper · 2017 · IET Nanobiotechnology

In vitro germination and biochemical profiling of citrus reticulata in response to green synthesised zinc and copper nanoparticles

Mubashir Hussain; Naveed Iqbal Raja; Zia‐Ur‐Rehman Mashwani; et al.

9
claims
16
verbatim quotes
45
citations
2
research areas
✓
Open access

Abstract

Green synthesis of nanoparticles by using plants is an emerging class of nanobiotechnology. It revolutionizes all the fields of nanobiotechnology by synthesizing chemical‐free nanoparticles for various purposes. In the present study, zinc and copper nanoparticles were synthesized by using the white leaves of Allium cepa and further characterized through Zeta analyzer and Scanning electron microscopy. Zeta analyzer elucidated that zinc nanoparticles ranged from 8‐32 nm while copper nanoparticles ranged from 15‐30 nm. Scanning electron microscopy clarified that zinc nanoparticles were irregular while copper nanoparticles were spherical in shape. The effects of green synthesized nanoparticles were evaluated on the germination frequency and biochemical parameters of plant tissues. The nucellus tissues were inoculated on Murashige and Skoog (MS) medium augmented with 30 µg/ml suspension of zinc and copper nanoparticles. Green synthesized nanoparticles enhanced the in vitro germination parameters because of their low toxicity and high efficacy. Significant results were obtained for germination parameters in response to the applications of zinc nanoparticles as compared to copper nanoparticles. These nanoparticles could also induce stress in plantlets by manipulating the endogenous mechanism as a result various defence compounds are produced which have potential in treating various human ailments. Copper nanoparticles showed higher toxicity as compared to zinc nanoparticles and triggered the production of antioxidative enzymes and non‐ enzymatic compounds.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 9

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Zeta analysis showed zinc nanoparticles ranged from 8-32 nm.

Outcome
zinc nanoparticlese0
Value
8‐32 nme0

Verbatim

e0

“zinc nanoparticles ranged from 8‐32 nm”

✓ located verbatim in the source · abstract, characters 434–472 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Zeta analysis showed copper nanoparticles ranged from 15-30 nm.

Outcome
copper nanoparticlese0
Value
15‐30 nme0

Verbatim

e0

“copper nanoparticles ranged from 15‐30 nm”

✓ located verbatim in the source · abstract, characters 479–520 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Scanning electron microscopy showed zinc nanoparticles were irregular in shape.

Taxon
Allium cepaAllium cepae1
Outcome
zinc nanoparticles were irregulare0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“zinc nanoparticles were irregular”

✓ located verbatim in the source · abstract, characters 566–599 · show in text ↑
e1

“Allium cepa”

✓ located verbatim in the source · abstract, characters 310–321 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Observationobservedoriginal resultmeasured

Claim · model-written summary

Scanning electron microscopy showed copper nanoparticles were spherical in shape.

Taxon
Allium cepaAllium cepae1
Outcome
copper nanoparticles were spherical in shapee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“copper nanoparticles were spherical in shape”

✓ located verbatim in the source · abstract, characters 606–650 · show in text ↑
e1

“Allium cepa”

✓ located verbatim in the source · abstract, characters 310–321 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Green synthesized nanoparticles enhanced in vitro germination parameters of Citrus reticulata nucellus tissues.

Taxon
citrus reticulataCitrus reticulatat0
Treatment
Green synthesized nanoparticlese0
Outcome
in vitro germination parameterse0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Green synthesized nanoparticles enhanced the in vitro germination parameters”

✓ located verbatim in the source · abstract, characters 929–1005 · show in text ↑
t0

“citrus reticulata”

✓ located verbatim in the source · title, characters 50–67 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zinc nanoparticles gave significant germination results compared with copper nanoparticles.

Taxon
citrus reticulataCitrus reticulatat0
Treatment
zinc nanoparticlese0
Outcome
germination parameterse0
Comparator
copper nanoparticlese0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Significant results were obtained for germination parameters in response to the applications of zinc nanoparticles as compared to copper nanoparticles”

✓ located verbatim in the source · abstract, characters 1055–1205 · show in text ↑
t0

“citrus reticulata”

✓ located verbatim in the source · title, characters 50–67 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper nanoparticles showed higher toxicity than zinc nanoparticles.

Taxon
citrus reticulataCitrus reticulatat0
Treatment
Copper nanoparticlese0
Outcome
toxicitye0
Direction
highere0
Comparator
zinc nanoparticlese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Copper nanoparticles showed higher toxicity as compared to zinc nanoparticles”

✓ located verbatim in the source · abstract, characters 1414–1491 · show in text ↑
t0

“citrus reticulata”

✓ located verbatim in the source · title, characters 50–67 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Copper nanoparticles triggered the production of antioxidative enzymes and non-enzymatic compounds.

Taxon
citrus reticulataCitrus reticulatat0
Treatment
Copper nanoparticlese0
Outcome
production of antioxidative enzymese0
Direction
triggerede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Copper nanoparticles showed higher toxicity as compared to zinc nanoparticles and triggered the production of antioxidative enzymes and non‐ enzymatic compounds”

✓ located verbatim in the source · abstract, characters 1414–1574 · show in text ↑
t0

“citrus reticulata”

✓ located verbatim in the source · title, characters 50–67 · show in text ↑
Mechanismhypothesisoriginal resultmeasured

Claim · model-written summary

The nanoparticles could induce stress in plantlets by manipulating the endogenous mechanism, leading to production of various defence compounds.

Taxon
citrus reticulataCitrus reticulatat0
Treatment
These nanoparticlese0
Outcome
stress in plantletse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“These nanoparticles could also induce stress in plantlets by manipulating the endogenous mechanism as a result various defence compounds are produced”

✓ located verbatim in the source · abstract, characters 1207–1356 · show in text ↑
t0

“citrus reticulata”

✓ located verbatim in the source · title, characters 50–67 · show in text ↑

zinc atom · growth · Citrus reticulata (tangerine)Results agree

11 papers: 11 positive, 0 negative or no effect

10 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

nanoparticle · growth · Citrus reticulata (tangerine)Results agree

7 papers: 7 positive, 0 negative or no effect

6 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.