Abstract
WRKY transcription factors (TFs) play crucial roles in plant resistance responses to pathogens. Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat (Triticum aestivum) worldwide. In this study, the two WRKY genes TaWRKY49 and TaWRKY62 were originally identified in association with high-temperature seedling-plant resistance to Pst (HTSP) resistance in wheat cultivar Xiaoyan 6 by RNA-seq. Interestingly, the expression levels of TaWRKY49 and TaWRKY62 were down- and up-regulated, respectively, during HTSP resistance in response to Pst. Silencing of TaWRKY49 enhanced whereas silencing TaWRKY62 reduced HTSP resistance. The enhanced resistance observed on leaves following the silencing of TaWRKY49 was coupled with increased expression of salicylic acid (SA)- and jasmonic acid (JA)-responsive genes TaPR1.1 and TaAOS, as well as reactive oxygen species (ROS)-associated genes TaCAT and TaPOD; whereas the ethylene (ET)-responsive gene TaPIE1 was suppressed. The decreased resistance observed on leaves following TaWRKY62 silencing was associated with increased expression of TaPR1.1 and TaPOD, and suppression of TaAOS and TaPIE1. Furthermore, SA, ET, MeJA (methyl jasmonate), hydrogen peroxide (H2O2) and abscisic acid (ABA) treatments increased TaWRKY62 expression. On the other hand, MeJA did not affect the expression of TaWRKY49, and H2O2 reduced TaWRKY49 expression. In conclusion, TaWRKY49 negatively regulates while TaWRKY62 positively regulates wheat HTSP resistance to Pst by differential regulation of SA-, JA-, ET and ROS-mediated signaling.
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Claims 16
Claim · model-written summary
TaWRKY49 and TaWRKY62 were identified in association with high-temperature seedling resistance to Pst in Xiaoyan 6.
- Taxon
- wheat
Triticum aestivume0 - Material
- Xiaoyan 6e0
- Interactor
- Pste0pathogen
- Gene
- TaWRKY49 and TaWRKY62e0
- Outcome
- high-temperature seedling-plant resistance to Pst (HTSP) resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two WRKY genes TaWRKY49 and TaWRKY62 were originally identified in association with high-temperature seedling-plant resistance to Pst (HTSP) resistance in wheat cultivar Xiaoyan 6”
Claim · model-written summary
TaWRKY49 expression was down-regulated during HTSP resistance in response to Pst.
- Taxon
- wheatTriticum aestivume1
- Interactor
- Pste0pathogen
- Gene
- TaWRKY49e0
- Outcome
- expression levels of TaWRKY49e0
- Direction
- down-e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of TaWRKY49 and TaWRKY62 were down- and up-regulated, respectively, during HTSP resistance in response to Pst”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
TaWRKY62 expression was up-regulated during HTSP resistance in response to Pst.
- Taxon
- wheatTriticum aestivume1
- Interactor
- Pste0pathogen
- Gene
- TaWRKY62e0
- Outcome
- expression levels of TaWRKY49 and TaWRKY62e0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of TaWRKY49 and TaWRKY62 were down- and up-regulated, respectively, during HTSP resistance in response to Pst”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
Silencing TaWRKY49 enhanced HTSP resistance.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Silencing of TaWRKY49e0
- Gene
- TaWRKY49e0
- Outcome
- HTSP resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of TaWRKY49 enhanced whereas silencing TaWRKY62 reduced HTSP resistance.”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
Silencing TaWRKY62 reduced HTSP resistance.
- Taxon
- wheatTriticum aestivume1
- Treatment
- silencing TaWRKY62e0
- Gene
- TaWRKY62e0
- Outcome
- HTSP resistancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of TaWRKY49 enhanced whereas silencing TaWRKY62 reduced HTSP resistance.”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
TaWRKY49 silencing increased expression of TaPR1.1, TaAOS, TaCAT and TaPOD.
- Taxon
- wheatTriticum aestivume1
- Treatment
- silencing of TaWRKY49e0
- Gene
- TaPR1.1 and TaAOSe0
- Outcome
- expression of salicylic acid (SA)- and jasmonic acid (JA)-responsive genes TaPR1.1 and TaAOS, as well as reactive oxygen species (ROS)-associated genes TaCAT and TaPODe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of TaWRKY49 was coupled with increased expression of salicylic acid (SA)- and jasmonic acid (JA)-responsive genes TaPR1.1 and TaAOS, as well as reactive oxygen species (ROS)-associated genes TaCAT and TaPOD”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
TaPIE1 expression was suppressed after TaWRKY49 silencing.
- Taxon
- wheatTriticum aestivume2
- Treatment
- silencing of TaWRKY49e1
- Gene
- TaPIE1e0
- Outcome
- gene TaPIE1e0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ethylene (ET)-responsive gene TaPIE1 was suppressed”
e1“silencing of TaWRKY49”
e2“wheat (Triticum aestivum)”
Claim · model-written summary
TaWRKY62 silencing increased TaPR1.1 and TaPOD expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- TaWRKY62 silencinge0
- Gene
- TaPR1.1 and TaPODe0
- Outcome
- expression of TaPR1.1 and TaPODe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY62 silencing was associated with increased expression of TaPR1.1 and TaPOD”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
TaWRKY62 silencing suppressed TaAOS and TaPIE1 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- TaWRKY62 silencinge0
- Gene
- TaAOS and TaPIE1e0
- Outcome
- TaAOS and TaPIE1e0
- Direction
- suppressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY62 silencing was associated with increased expression of TaPR1.1 and TaPOD, and suppression of TaAOS and TaPIE1”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
SA, ET and MeJA treatments increased TaWRKY62 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- SA, ET, MeJA (methyl jasmonate)e0
- Gene
- TaWRKY62e0
- Outcome
- TaWRKY62 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA, ET, MeJA (methyl jasmonate), hydrogen peroxide (H2O2) and abscisic acid (ABA) treatments increased TaWRKY62 expression”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
Hydrogen peroxide treatment increased TaWRKY62 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- hydrogen peroxide (H2O2)e0
- Gene
- TaWRKY62e0
- Outcome
- TaWRKY62 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydrogen peroxide (H2O2) and abscisic acid (ABA) treatments increased TaWRKY62 expression”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
ABA treatment increased TaWRKY62 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- abscisic acid (ABA)e0
- Gene
- TaWRKY62e0
- Outcome
- TaWRKY62 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“abscisic acid (ABA) treatments increased TaWRKY62 expression”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
MeJA did not affect TaWRKY49 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- MeJAe0
- Gene
- TaWRKY49e0
- Outcome
- expression of TaWRKY49e0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA did not affect the expression of TaWRKY49”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
H2O2 reduced TaWRKY49 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- H2O2e0
- Gene
- TaWRKY49e0
- Outcome
- TaWRKY49 expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2 reduced TaWRKY49 expression”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
The authors conclude TaWRKY49 negatively regulates wheat HTSP resistance to Pst.
- Taxon
- wheatTriticum aestivume1
- Interactor
- Pste0pathogen
- Gene
- TaWRKY49e0
- Outcome
- wheat HTSP resistance to Pste0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY49 negatively regulates while TaWRKY62 positively regulates wheat HTSP resistance to Pst”
e1“wheat (Triticum aestivum)”
Claim · model-written summary
The authors conclude TaWRKY62 positively regulates wheat HTSP resistance to Pst.
- Taxon
- wheatTriticum aestivume1
- Interactor
- Pste0pathogen
- Gene
- TaWRKY62e0
- Outcome
- wheat HTSP resistance to Pste0
- Direction
- positively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY49 negatively regulates while TaWRKY62 positively regulates wheat HTSP resistance to Pst”
e1“wheat (Triticum aestivum)”
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