Abstract
Background and Aims: Certain micro-organisms can improve plant protection against pathogens. The protective effect may be direct, e.g. due to antibiotic compounds, or indirect, by priming of plant defence as induced systemic resistance (ISR). The plant growth-promoting rhizobacterium Bacillus amyloliquefaciens UCMB5113 shows potential for disease management of oilseed rape. To investigate the mode of action of this protection, especially in relation to jasmonic acid-dependent ISR, Bacillus UCMB5113 was tested with Arabidopsis thaliana mutants and several important fungal pathogens of Brassica species. Methods: Secreted lipopeptide fractions from Bacillus UCMB5113, together with synthetic peptide mimics, were evaluated for their effects on fungal phytopathogens and A. thaliana . The structures of secreted lipopeptides were analysed using mass spectrometry. Plant mutants and reporter lines were used to identify signalling steps involved in disease suppression by lipopeptides. Key Results: In plate tests Bacillus UCMB5113 and lipopeptide extracts suppressed growth of several fungal pathogens infecting Brassica plants. Separation of secreted lipopeptides using reversed-phase high-performance liquid chromatography revealed several fractions that inhibited fungal growth. Analysis by mass spectrometry identified the most potent compounds as novel linear forms of antifungal fengycins, with synthetic peptide mimics confirming the biological activity. Application of the lipopeptide extracts on Arabidopsis roots provided systemic protection against Alternaria brassicicola on leaves. Arabidopsis signalling mutants and PDF1.2 and VSP2 promoter-driven GUS lines indicated that the lipopeptide fraction involved jasmonic-acid-dependent host responses for suppression of fungal growth indicative of ISR. Conclusions: The ability of Bacillus UCMB5113 to counteract pathogens using both antagonistic lipopeptides and through ISR provides a promising tool for sustainable crop production.
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Claims 7
Claim · model-written summary
Bacillus UCMB5113 and lipopeptide extracts suppressed growth of several fungal pathogens infecting Brassica plants in plate tests.
- Treatment
- lipopeptide extractse1
- Interactor
- fungal pathogense1pathogen
- Second interactor
- Bacillus UCMB5113e1biocontrol_agent
- Outcome
- growth of several fungal pathogense1
- Direction
- suppressede1
- Setting
- plate testse1
BrassicaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In plate tests Bacillus UCMB5113 and lipopeptide extracts suppressed growth of several fungal pathogens infecting Brassica plants”
Claim · model-written summary
Several reversed-phase HPLC fractions of secreted lipopeptides inhibited fungal growth.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Separation of secreted lipopeptides using reversed-phase high-performance liquid chromatography revealed several fractions that inhibited fungal growth”
t0“biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides”
Claim · model-written summary
Mass spectrometry identified the most potent compounds as novel linear forms of antifungal fengycins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Analysis by mass spectrometry identified the most potent compounds as novel linear forms of antifungal fengycins”
t0“biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides”
Claim · model-written summary
Synthetic peptide mimics confirmed the biological activity of the identified compounds.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“with synthetic peptide mimics confirming the biological activity”
t0“biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides”
Claim · model-written summary
Lipopeptide extracts applied to Arabidopsis roots provided systemic protection against Alternaria brassicicola on leaves.
- Taxon
- Arabidopsis
Arabidopsise4 - Organ / stage
- leavese4
- Treatment
- lipopeptide extractse4
- Interactor
- Alternaria brassicicola
Alternaria brassicicolae4pathogen - Outcome
- systemic protection against Alternaria brassicicolae4
- Direction
- decreaseinferrede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Application of the lipopeptide extracts on Arabidopsis roots provided systemic protection against Alternaria brassicicola on leaves”
Claim · model-written summary
The lipopeptide fraction involved jasmonic-acid-dependent host responses in suppressing fungal growth, indicative of induced systemic resistance (ISR).
- Taxon
- ArabidopsisArabidopsise5
- Treatment
- lipopeptide fractione5
- Gene
- PDF1.2 and VSP2e5
- Outcome
- suppression of fungal growthe5
- Qualifier
- jasmonic-acid-dependent host responsese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Arabidopsis signalling mutants and PDF1.2 and VSP2 promoter-driven GUS lines indicated that the lipopeptide fraction involved jasmonic-acid-dependent host responses for suppression of fungal growth indicative of ISR”
Claim · model-written summary
Bacillus UCMB5113 counteracting pathogens through antagonistic lipopeptides and ISR is a promising tool for sustainable crop production.
- Outcome
- promising tool for sustainable crop productione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The ability of Bacillus UCMB5113 to counteract pathogens using both antagonistic lipopeptides and through ISR provides a promising tool for sustainable crop production”
t0“biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides”
The same questions in other papers
botanical · growth · BrassicaPositive and negative results
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