Abstract
In eukaryotes, RNA silencing, mediated by small interfering RNAs, is an evolutionarily widespread and versatile silencing mechanism that plays an important role in various biological processes. Increasing evidences suggest that various components of RNA silencing pathway are involved in plant defense machinery against microbial pathogens in Arabidopsis thaliana. Here, we show genetic and molecular evidence that Arabidopsis SDE5 is required to generate an effective resistance against the biotrophic bacteria Pseudomonas syringae pv. tomato DC3000 and for susceptibility to the necrotrophic bacteria Erwinia caratovora pv. caratovora. SDE5, encodes a putative mRNA export factor that is indispensable for transgene silencing and the production of trans-acting siRNAs. SDE5 expression is rapidly induced by exogenous application of phytohormone salicylic acid (SA), methyl jasmonate (MeJA), phytopathogenic bacteria, and flagellin. We further report that SDE5 is involved in basal plant defense and mRNA export. Our genetic data suggests that SDE5 and Nonexpressor of PR Gene1 (NPR1) may contribute to the same SA-signaling pathway. However, SDE5 over-expressing transgenic plant exhibits reduced defense responsive phenotype after flagellin treatment. Taken together, these results support the conclusion that SDE5 contributes to plant innate immunity in Arabidopsis.
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Claims 12
Claim · model-written summary
SDE5 was required for effective resistance to Pseudomonas syringae pv. tomato DC3000.
- Taxon
- Arabidopsis
Arabidopsise0 - Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Gene
- SDE5e0
- Outcome
- effective resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis SDE5 is required to generate an effective resistance against the biotrophic bacteria Pseudomonas syringae pv. tomato DC3000”
Claim · model-written summary
SDE5 was required for susceptibility to Erwinia caratovora pv. caratovora.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- Erwinia caratovora pv. caratovorae0pathogen
- Gene
- SDE5e0
- Outcome
- susceptibilitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis SDE5 is required to generate an effective resistance against the biotrophic bacteria Pseudomonas syringae pv. tomato DC3000 and for susceptibility to the necrotrophic bacteria Erwinia caratovora pv. caratovora.”
Claim · model-written summary
SDE5 encodes a putative mRNA export factor essential for transgene silencing.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- transgene silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5, encodes a putative mRNA export factor that is indispensable for transgene silencing and the production of trans-acting siRNAs.”
e1“Arabidopsis SDE5”
Claim · model-written summary
SDE5 is essential for production of trans-acting siRNAs.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- production of trans-acting siRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5, encodes a putative mRNA export factor that is indispensable for transgene silencing and the production of trans-acting siRNAs.”
e1“Arabidopsis SDE5”
Claim · model-written summary
Salicylic acid rapidly induced SDE5 expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- salicylic acid (SA)e0
- Gene
- SDE5e0
- Outcome
- SDE5 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 expression is rapidly induced by exogenous application of phytohormone salicylic acid (SA), methyl jasmonate (MeJA), phytopathogenic bacteria, and flagellin.”
e1“Arabidopsis SDE5”
Claim · model-written summary
Methyl jasmonate rapidly induced SDE5 expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- methyl jasmonate (MeJA)e0
- Gene
- SDE5e0
- Outcome
- SDE5 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 expression is rapidly induced by exogenous application of phytohormone salicylic acid (SA), methyl jasmonate (MeJA), phytopathogenic bacteria, and flagellin.”
e1“Arabidopsis SDE5”
Claim · model-written summary
Phytopathogenic bacteria rapidly induced SDE5 expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- phytopathogenic bacteriae0
- Gene
- SDE5e0
- Outcome
- SDE5 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 expression is rapidly induced by exogenous application of phytohormone salicylic acid (SA), methyl jasmonate (MeJA), phytopathogenic bacteria, and flagellin.”
e1“Arabidopsis SDE5”
Claim · model-written summary
Flagellin rapidly induced SDE5 expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- flagelline0
- Gene
- SDE5e0
- Outcome
- SDE5 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 expression is rapidly induced by exogenous application of phytohormone salicylic acid (SA), methyl jasmonate (MeJA), phytopathogenic bacteria, and flagellin.”
e1“Arabidopsis SDE5”
Claim · model-written summary
SDE5 was implicated in basal plant defense.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- basal plant defensee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 is involved in basal plant defense and mRNA export.”
e1“Arabidopsis SDE5”
Claim · model-written summary
SDE5 was implicated in mRNA export.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5e0
- Outcome
- mRNA exporte0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 is involved in basal plant defense and mRNA export.”
e1“Arabidopsis SDE5”
Claim · model-written summary
Genetic data suggested SDE5 and NPR1 may contribute to the same SA-signaling pathway.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SDE5 and Nonexpressor of PR Gene1 (NPR1)e0
- Outcome
- same SA-signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 and Nonexpressor of PR Gene1 (NPR1) may contribute to the same SA-signaling pathway.”
e1“Arabidopsis SDE5”
Claim · model-written summary
SDE5 overexpression reduced defense responsiveness after flagellin treatment.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- flagellin treatmente0
- Gene
- SDE5e0
- Outcome
- defense responsive phenotypee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDE5 over-expressing transgenic plant exhibits reduced defense responsive phenotype after flagellin treatment.”
e1“Arabidopsis SDE5”
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See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.