Abstract
Vitis species, including grapevine, produce a class of secondary metabolites called stilbenes that are important for plant disease resistance and can have positive effects on human health. Mitogen-activated protein kinase (MAPK) signaling cascades not only play key roles in plant defense responses but also contribute to stilbene biosynthesis in grapevine. MAPKKKs function at the upper level of the MAPK network and initiate signaling through this pathway. In this study, a Raf-like MAPKKK gene, VqMAPKKK38, was identified and functionally characterized from the Chinese wild grapevine V. quinquangularis accession ‘Danfeng-2’. We observed that VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA). In addition, based on both transient overexpression and gene suppression of VqMAPKKK38 in grapevine leaves, we found that VqMAPKKK38 positively regulates stilbene synthase transcription and stilbene accumulation probably by mediating the activation of the transcription factor MYB14. In addition, both hydrogen peroxide (H2O2) and calcium influx activated VqMAPKKK38 expression and stilbene biosynthesis, which suggests that VqMAPKKK38 may be involved in the calcium signaling and ROS signaling pathways.
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Claims 13
Claim · model-written summary
A Raf-like MAPKKK gene, VqMAPKKK38, was identified and functionally characterized from the wild grapevine V. quinquangularis accession Danfeng-2.
- Taxon
- V. quinquangularis
Vitis quinquangularise1 - Material
- Danfeng-2e1
- Gene
- VqMAPKKK38e1
- Outcome
- a Raf-like MAPKKK genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a Raf-like MAPKKK gene, VqMAPKKK38, was identified and functionally characterized from the Chinese wild grapevine V. quinquangularis accession ‘Danfeng-2’”
Claim · model-written summary
Powdery mildew infection elevated VqMAPKKK38 transcript levels in grapevine.
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e2“VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA)”
Claim · model-written summary
High salinity conditions elevated VqMAPKKK38 transcript levels in grapevine.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e2“VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA)”
Claim · model-written summary
Chilling stresses elevated VqMAPKKK38 transcript levels in grapevine.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e2“VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA)”
Claim · model-written summary
Salicylic acid treatment elevated VqMAPKKK38 transcript levels in grapevine.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e2“VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA)”
Claim · model-written summary
Methyl jasmonate treatment elevated VqMAPKKK38 transcript levels in grapevine.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e2“VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA)”
Claim · model-written summary
Ethylene treatment elevated VqMAPKKK38 transcript levels in grapevine.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e2“VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA)”
Claim · model-written summary
Abscisic acid treatment elevated VqMAPKKK38 transcript levels in grapevine.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e2“VqMAPKKK38 transcript levels were elevated by powdery mildew infection, high salinity conditions and chilling stresses, as well as in response to treatments by the hormones salicylic acid (SA), methyl jasmonate (MeJA), ethylene (Eth) and abscisic acid (ABA)”
Claim · model-written summary
Hydrogen peroxide activated VqMAPKKK38 expression and stilbene biosynthesis in grapevine.
- Treatment
- hydrogen peroxide (H2O2)e4
- Gene
- VqMAPKKK38e4
- Outcome
- VqMAPKKK38 expression and stilbene biosynthesise4
- Direction
- activatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e4“both hydrogen peroxide (H2O2) and calcium influx activated VqMAPKKK38 expression and stilbene biosynthesis”
Claim · model-written summary
Calcium influx activated VqMAPKKK38 expression and stilbene biosynthesis in grapevine.
- Treatment
- calcium influxe4
- Gene
- VqMAPKKK38e4
- Outcome
- VqMAPKKK38 expression and stilbene biosynthesise4
- Direction
- activatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e4“both hydrogen peroxide (H2O2) and calcium influx activated VqMAPKKK38 expression and stilbene biosynthesis”
Claim · model-written summary
Transient overexpression and gene suppression of VqMAPKKK38 showed that it positively regulates stilbene synthase transcription and stilbene accumulation in grapevine leaves.
- Treatment
- transient overexpression and gene suppressione3
- Gene
- VqMAPKKK38e3
- Outcome
- stilbene synthase transcription and stilbene accumulatione3
- Direction
- positively regulatese3
- Qualifier
- probablye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e3“based on both transient overexpression and gene suppression of VqMAPKKK38 in grapevine leaves, we found that VqMAPKKK38 positively regulates stilbene synthase transcription and stilbene accumulation probably by mediating the activation of the transcription factor MYB14”
Claim · model-written summary
VqMAPKKK38 probably acts by mediating activation of the transcription factor MYB14 in grapevine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e3“based on both transient overexpression and gene suppression of VqMAPKKK38 in grapevine leaves, we found that VqMAPKKK38 positively regulates stilbene synthase transcription and stilbene accumulation probably by mediating the activation of the transcription factor MYB14”
Claim · model-written summary
VqMAPKKK38 may be involved in calcium signaling and ROS signaling pathways in grapevine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitis species, including grapevine”
e5“VqMAPKKK38 may be involved in the calcium signaling and ROS signaling pathways”
The same questions in other papers
hydrogen peroxide · molecular: expression · VitisResults agree
2 papers in the same direction as this paper
- positive
- positive
calcium atom · molecular: expression · VitisResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.