Abstract
Abstract Discovering the key genes responsible for deep‐sowing germination is very meaningful to cultivate maize varieties with strong germination ability in soil. In this study, Zm MYB 59 gene whose functions remain unknown was successfully cloned. Subcellular localization showed that Zm MYB 59 was localized in the nucleus. By analysing cis‐acting elements of its promoter sequence, many elements of MYB binding domain responsive to hormones (particularly gibberellin, GA ) were found. On this basis, Zm MYB 59 expression in different sowing depths, germination stages, tissues and treatments was analysed by real‐time PCR . Zm MYB 59 expression levels in embryos or seedlings were significantly decreased with the increase in sowing depth at 2 days or 4 days after sowing ( DAS ). Further analysis showed that its expression in mesocotyls was also significantly downregulated with the increase in sowing depth at both 6 DAS and 8 DAS . Similarly, seed soaking with 10 ‐5 M GA 3 inhibited Zm MYB 59 expression in mesocotyls at 3 DAS . These results suggest that Zm MYB 59 may play a negative regulatory role in seed germination in deep soil and the regulation is involved in GA signalling pathway.
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Claims 7
Claim · model-written summary
ZmMYB59 was cloned.
Verbatim
e0“Zm MYB 59 gene whose functions remain unknown was successfully cloned”
Claim · model-written summary
ZmMYB59 localized to the nucleus.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zm MYB 59 was localized in the nucleus”
t0“maize”
Claim · model-written summary
The promoter contained numerous hormone responsive MYB binding elements, especially for gibberellin.
- Outcome
- many elements of MYB binding domain responsive to hormonese0
- Qualifier
- particularly gibberellin, GAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“many elements of MYB binding domain responsive to hormones (particularly gibberellin, GA ) were found”
t0“maize”
Claim · model-written summary
ZmMYB59 expression in embryos or seedlings fell significantly as sowing depth increased at 2 or 4 days.
- Organ / stage
- embryos or seedlingse0
- Treatment
- increase in sowing depthe0
- Gene
- Zm MYB 59e0
- Outcome
- Zm MYB 59 expression levelse0
- Direction
- decreasede0
- Qualifier
- 2 days or 4 days after sowinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zm MYB 59 expression levels in embryos or seedlings were significantly decreased with the increase in sowing depth at 2 days or 4 days after sowing ( DAS )”
t0“maize”
Claim · model-written summary
Mesocotyl ZmMYB59 expression fell significantly with increasing sowing depth at both 6 and 8 days.
- Treatment
- increase in sowing depthe0
- Gene
- Zm MYB 59e1
- Outcome
- its expression in mesocotylse0
- Direction
- downregulatede0
- Qualifier
- at both 6 DAS and 8 DASe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression in mesocotyls was also significantly downregulated with the increase in sowing depth at both 6 DAS and 8 DAS”
e1“Zm MYB 59 expression levels in embryos”
t0“maize”
Claim · model-written summary
GA3 seed soaking inhibited mesocotyl ZmMYB59 expression at 3 days.
- Organ / stage
- mesocotylse0
- Treatment
- seed soaking with 10 ‐5 M GA 3e0
- Outcome
- Zm MYB 59 expressione0
- Direction
- inhibitede0
- Qualifier
- at 3 DASe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed soaking with 10 ‐5 M GA 3 inhibited Zm MYB 59 expression in mesocotyls at 3 DAS”
t0“maize”
Claim · model-written summary
The authors suggest ZmMYB59 may negatively regulate germination in deep soil through GA signaling.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zm MYB 59 may play a negative regulatory role in seed germination in deep soil and the regulation is involved in GA signalling pathway”
t0“maize”
The same questions in other papers
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.