Abstract
An in-house library of more than 3000 extracts of plant and fungal origin was screened against some major plant pathogens. As one of the hits, an ethyl acetate extract from inflorescences of Verbesina lanata showed significant inhibitory activity in vitro against grapevine downy mildew ( Plasmopara viticola ), with a MIC 100 value of 35 μg/mL. An emulsifiable concentrate formulation with 50 mg/g of the extract was developed for in vivo evaluation. A suspension of the formulation containing 1 mg/mL of extract lowered leaf surface infection of grapevine seedling by 82% compared to the nontreated control. With the aid of HPLC-based activity profiling, the antifungal activity was correlated with a series of lipophilic compounds. Preparative isolation by a combination of chromatographic techniques afforded 16 eudesmane sesquiterpenes including eight new congeners. Nine compounds were obtained in sufficient quantities to be tested in vitro and were found to inhibit the zoospore activity of P. viticola with MIC 100 values ranging from 4 to 50 μg/mL. The two major compounds, 6β-cinnamoyloxy-4β,9β,15-trihydroxyeudesmane ( 9 ) and 6β-cinnamoyloxy-1β,15-dihydroxyeudesm-4-en-3-one ( 13 ), showed MIC 100 values of 5 and 31 μg/mL, respectively.
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Claims 7
Claim · model-written summary
Verbesina lanata extract inhibited Plasmopara viticola in vitro with MIC 100 of 35 μg/mL.
- Treatment
- ethyl acetate extract from inflorescences of Verbesina lanatae0
- Interactor
- Plasmopara viticola
Plasmopara viticolae0pathogen - Outcome
- inhibitory activity in vitro against grapevine downy mildewe0
- Direction
- inhibitorye0
- Value
- 35 μg/mLe0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an ethyl acetate extract from inflorescences of Verbesina lanata showed significant inhibitory activity in vitro against grapevine downy mildew ( Plasmopara viticola ), with a MIC 100 value of 35 μg/mL.”
Claim · model-written summary
Extract formulation lowered grapevine seedling leaf infection by 82% versus untreated control.
- Organ / stage
- seedlinge0
- Treatment
- suspension of the formulation containing 1 mg/mL of extracte0
- Interactor
- Plasmopara viticolaPlasmopara viticolae1pathogen
- Outcome
- leaf surface infection of grapevine seedlinge0
- Direction
- lowerede0
- Value
- 82%e0
- Comparator
- nontreated controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A suspension of the formulation containing 1 mg/mL of extract lowered leaf surface infection of grapevine seedling by 82% compared to the nontreated control.”
t0“Grapevine Downy Mildew”
t1“Grapevine Downy Mildew”
e1“grapevine downy mildew ( Plasmopara viticola )”
Claim · model-written summary
Antifungal activity correlated with lipophilic compounds.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the antifungal activity was correlated with a series of lipophilic compounds.”
t0“Grapevine”
t1“Grapevine”
Claim · model-written summary
Isolation yielded 16 eudesmane sesquiterpenes, including eight new congeners.
- Taxon
- Verbesina lanataVerbesina lanatat0
- Outcome
- eudesmane sesquiterpenese0
- Value
- 16e0
- Qualifier
- eight new congenerse0
Verbatim
e0“Preparative isolation by a combination of chromatographic techniques afforded 16 eudesmane sesquiterpenes including eight new congeners.”
t0“Verbesina lanata”
t1“Verbesina lanata”
Claim · model-written summary
Nine isolated compounds inhibited P. viticola zoospore activity with MIC 100 values of 4 to 50 μg/mL.
- Treatment
- Nine compoundse0
- Interactor
- P. viticolaPlasmopara viticolae0pathogen
- Outcome
- zoospore activity of P. viticolae0
- Direction
- inhibite0
- Value
- 4 to 50 μg/mLe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nine compounds were obtained in sufficient quantities to be tested in vitro and were found to inhibit the zoospore activity of P. viticola with MIC 100 values ranging from 4 to 50 μg/mL.”
t0“Grapevine”
t1“Grapevine”
Claim · model-written summary
Compound 9 inhibited P. viticola at MIC 100 of 5 μg/mL.
- Treatment
- 6β-cinnamoyloxy-4β,9β,15-trihydroxyeudesmanee0
- Interactor
- P. viticolaPlasmopara viticolae1pathogen
- Outcome
- MIC 100e0
- Value
- 5e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6β-cinnamoyloxy-4β,9β,15-trihydroxyeudesmane ( 9 ) and 6β-cinnamoyloxy-1β,15-dihydroxyeudesm-4-en-3-one ( 13 ), showed MIC 100 values of 5 and 31 μg/mL, respectively.”
t0“Grapevine”
t1“Grapevine”
e1“zoospore activity of P. viticola”
Claim · model-written summary
Compound 13 inhibited P. viticola at MIC 100 of 31 μg/mL.
- Treatment
- 6β-cinnamoyloxy-1β,15-dihydroxyeudesm-4-en-3-onee0
- Interactor
- P. viticolaPlasmopara viticolae1pathogen
- Outcome
- MIC 100e0
- Value
- 31 μg/mLe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6β-cinnamoyloxy-4β,9β,15-trihydroxyeudesmane ( 9 ) and 6β-cinnamoyloxy-1β,15-dihydroxyeudesm-4-en-3-one ( 13 ), showed MIC 100 values of 5 and 31 μg/mL, respectively.”
t0“Grapevine”
t1“Grapevine”
e1“zoospore activity of P. viticola”
The same questions in other papers
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- positive
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- positive
- positive
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.