Abstract
BYPASS1 (BPS1), which is a well-conserved gene in plants, is required for normal root and shoot development. In the absence of BPS1 gene function, Arabidopsis overproduces a mobile signalling compound (the BPS1 signal) in roots, and this transmissible signal arrests shoot growth and causes abnormal root development. In addition to the shoot and root meristem activities, the legumes also possess transient meristematic activity in root cortical cells during Rhizobium symbiosis. We explored the role of Phaseolus vulgaris BPS1 during nodule primordium development using an RNA-interference (RNAi) silencing approach. Our results show that upon Rhizobium infection, the PvBPS1-RNAi transgenic roots failed to induce cortical cell divisions without affecting the rhizobia-induced root hair curling and infection thread formation. The transcript accumulation of early nodulin genes, cell cyclins, and cyclin-dependent kinase genes was affected in RNAi lines. Interestingly, the PvBPS1-RNAi root nodule phenotype was partially rescued by exogenous application of fluridone, a carotenoid biosynthesis inhibitor, which was used because the carotenoids are precursors of BPS1 signalling molecules. Furthermore, we show that the PvBPS1 promoter was active in the nodule primordia. Together, our data show that PvBPS1 plays a vital role in the induction of meristematic activity in root cortical cells and in the establishment of nodule primordia during Phaseolus-Rhizobium symbiosis.
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Claims 5
Claim · model-written summary
PvBPS1-silenced roots failed to induce cortical cell divisions after Rhizobium infection.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarist0 - Material
- PvBPS1-RNAi transgenic rootse0
- Treatment
- Rhizobium infectione0
- Outcome
- cortical cell divisionse0
- Direction
- failed to inducee0
RhizobiumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“upon Rhizobium infection, the PvBPS1-RNAi transgenic roots failed to induce cortical cell divisions without affecting the rhizobia-induced root hair curling and infection thread formation.”
t0“Phaseolus vulgaris BPS1”
Claim · model-written summary
PvBPS1 silencing did not affect rhizobia-induced root hair curling or infection threads.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Material
- PvBPS1-RNAi transgenic rootse0
- Treatment
- Rhizobium infectione0
- Outcome
- rhizobia-induced root hair curling and infection thread formatione0
- Direction
- without affectinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“upon Rhizobium infection, the PvBPS1-RNAi transgenic roots failed to induce cortical cell divisions without affecting the rhizobia-induced root hair curling and infection thread formation.”
t0“Phaseolus vulgaris BPS1”
Claim · model-written summary
Silencing PvBPS1 affected early nodulin, cyclin and cyclin-dependent kinase transcripts.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Treatment
- RNAi linese0
- Outcome
- transcript accumulation of early nodulin genes, cell cyclins, and cyclin-dependent kinase genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript accumulation of early nodulin genes, cell cyclins, and cyclin-dependent kinase genes was affected in RNAi lines.”
t0“Phaseolus vulgaris BPS1”
Claim · model-written summary
Fluridone partly rescued the PvBPS1-RNAi root nodule phenotype.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Material
- PvBPS1-RNAie0
- Treatment
- exogenous application of fluridonee0
- Outcome
- root nodule phenotypee0
- Direction
- partially rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PvBPS1-RNAi root nodule phenotype was partially rescued by exogenous application of fluridone”
t0“Phaseolus vulgaris BPS1”
Claim · model-written summary
The PvBPS1 promoter was active in nodule primordia.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Organ / stage
- nodule primordiae0
- Gene
- PvBPS1e0
- Outcome
- promoter was activee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PvBPS1 promoter was active in the nodule primordia.”
t0“Phaseolus vulgaris BPS1”
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