Abstract
The present study was undertaken to develop a reproducible protocol for efficient in vitro callus initiation of chick pea (Cicer arietinum L.). The main objectives of this present study were to develop the optimal concentrations and combination of auxin and cytokinin for optimized callus induction from seeds as explants. Callus induction was initiated from seeds on MS media supplement, which varied according to the plant growth regulators treatment. Among the growth regulator combinations the highest rate of callus induction (85%) was observed in MS medium containing 2 mg L -1 of 2,4-Dichlorophenoxyacetic Acid (2,4-D), 2 mg L -1 Benzylaminopurine (BAP) showed higher percentage ( 63% ) of callus formation than 1- Naphthaleneacetic acid (NAA), which produced 49% of callus. There were significant differences in percentage of calli fresh/dry weights (g/jar) on the different initiation ( seven) medium used were the MS+2,4-D, MS+2,4-D +NAA+ BAP and MS+ BAP had the highest fresh/dry weights (g/jar) in both induction medium.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
MS medium with 2 mg L -1 2,4-D gave the highest callus induction rate, 85%.
- Taxon
- Chickpea
Cicer arietinumt0 - Treatment
- MS medium containing 2 mg L -1 of 2,4-Dichlorophenoxyacetic Acid (2,4-D)e0
- Outcome
- rate of callus inductione0
- Direction
- higheste0
- Value
- 85%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest rate of callus induction (85%) was observed in MS medium containing 2 mg L -1 of 2,4-Dichlorophenoxyacetic Acid (2,4-D)”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
BAP produced 63% callus formation, higher than NAA.
- Taxon
- ChickpeaCicer arietinumt0
- Treatment
- 2 mg L -1 Benzylaminopurine (BAP)e0
- Outcome
- callus formatione0
- Direction
- highere0
- Value
- 63%e0
- Qualifier
- 49%e0
- Comparator
- 1- Naphthaleneacetic acid (NAA)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2 mg L -1 Benzylaminopurine (BAP) showed higher percentage ( 63% ) of callus formation than 1- Naphthaleneacetic acid (NAA), which produced 49% of callus”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
NAA produced 49% callus formation.
- Taxon
- ChickpeaCicer arietinumt0
- Treatment
- 1- Naphthaleneacetic acid (NAA)e0
- Outcome
- calluse0
- Value
- 49%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1- Naphthaleneacetic acid (NAA), which produced 49% of callus”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
Fresh and dry callus weights differed significantly among initiation media, with MS+2,4-D, MS+2,4-D +NAA+ BAP and MS+ BAP highest.
- Taxon
- ChickpeaCicer arietinumt0
- Treatment
- MS+2,4-D, MS+2,4-D +NAA+ BAP and MS+ BAPe0
- Outcome
- fresh/dry weights (g/jar)e0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were significant differences in percentage of calli fresh/dry weights (g/jar) on the different initiation ( seven) medium used were the MS+2,4-D, MS+2,4-D +NAA+ BAP and MS+ BAP had the highest fresh/dry weights (g/jar)”
t0“Chickpea (Cicer arietinum L.)”
The same questions in other papers
2,4-D · growth · Cicer arietinum (chickpea)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →N-benzyladenine · growth · Cicer arietinum (chickpea)Positive and negative results
9 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
7 papers more in this group
See all 11 papers →in vitro · growth · Cicer arietinum (chickpea)Positive and negative results
13 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.