Abstract
The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes. Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects. Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield. In contrast, when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers. Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines. Increased amounts of intermediates of vitamin C biosynthesis were also observed. This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue. Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
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Claims 11
Claim · model-written summary
In transgenic potato lines, starch and amylose content were lower with StpsPPase down-regulation, which gave the most pronounced effects.
- Taxon
- potato
Solanum tuberosumt0 - Material
- transgenic linese1
- Treatment
- down-regulation of StpsPPasee1
- Gene
- StpsPPasee1
- Outcome
- Starch and amylose contente1
- Direction
- lowere1
- Qualifier
- the most pronounced effectse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e1“Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects”
Claim · model-written summary
In transgenic potato lines, granule size was smaller with StpsPPase down-regulation.
- Taxon
- potatoSolanum tuberosumt0
- Material
- transgenic linese1
- Treatment
- down-regulation of StpsPPasee1
- Gene
- StpsPPasee1
- Outcome
- granule sizee1
- Direction
- smallere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e1“Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects”
Claim · model-written summary
Single-gene down-regulation of StpsPPase or StNTT increased tuber number per plant.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Single-gene down-regulation of either StpsPPase or StNTTe2
- Gene
- StpsPPase or StNTTe2
- Outcome
- tuber numbers per plante2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e2“Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant”
Claim · model-written summary
Single-gene down-regulation of StpsPPase or StNTT gave higher fresh weight yield.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Single-gene down-regulation of either StpsPPase or StNTTe2
- Gene
- StpsPPase or StNTTe2
- Outcome
- fresh weight yielde3
- Direction
- highere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e2“Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant”
e3“higher fresh weight yield”
Claim · model-written summary
With both StpsPPase and StNTT inhibited simultaneously, some lines formed only a few, small and distorted tubers.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- both genes were inhibited simultaneouslye4
- Outcome
- tuberse4
- Direction
- decreaseinferrede4
- Qualifier
- some linese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e4“when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers”
Claim · model-written summary
Metabolite analysis revealed altered amounts of sugar intermediates in potato.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- amounts of sugar intermediatese5
- Qualifier
- alterede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e5“Analysis of metabolites revealed altered amounts of sugar intermediates”
Claim · model-written summary
StpsPPase down-regulation increased ADP-glucose content in the transgenic lines.
- Taxon
- potatoSolanum tuberosumt0
- Material
- StpsPPase linese6
- Treatment
- down-regulation of StpsPPasee1
- Gene
- StpsPPasee6
- Outcome
- ADP-glucose contente6
- Direction
- increasee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e1“with down-regulation of StpsPPase generating the most pronounced effects”
e6“a substantial increase in ADP-glucose content of the StpsPPase lines”
Claim · model-written summary
Intermediates of vitamin C biosynthesis were increased in the transgenic potato lines.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- amounts of intermediates of vitamin C biosynthesise7
- Direction
- Increasede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e7“Increased amounts of intermediates of vitamin C biosynthesis were also observed”
Claim · model-written summary
The authors suggest hydrolysis of pyrophosphate by a plastidial PPase is vital for functional starch accumulation in potato tubers.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- psPPasee0
- Outcome
- functional starch accumulation in potato tuberse0
- Qualifier
- hydrolysis of pyrophosphate (PPi)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e0“This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers”
Claim · model-written summary
No additional mechanism for consuming, hydrolysing or exporting PPi exists in the studied potato tissue.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- additional mechanism for consuming, hydrolysing, or exporting PPie0
- Qualifier
- in the studied tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e0“no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue”
Claim · model-written summary
Functional PPi hydrolysis combined with efficient ATP import is essential for potato tuber formation and development.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- tuber formation and developmente0
- Qualifier
- functional PPi hydrolysis in combination with efficient ATP importe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato”
e0“Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development”
The same questions in other papers
loss of function · yield · Solanum tuberosum (potato)Positive and negative results
3 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.