Abstract
In the spring oilseed rape (OSR) cultivar 'Mozart' grown under optimal N supply (NO) or mild N deficiency (NL) the transcriptome changes associated with progressing age until early senescence in developmentally old lower canopy leaves (leaf #4) and younger higher canopy leaves (leaf #8) were investigated. Twelve weeks old NO and NL plants appeared phenotypically and transcriptomically identical, but thereafter distinct nutrition-dependent differences in gene expression patterns in lower and upper canopy leaves emerged. In NO leaves #4 of 14-week-old compared to 13-week-old plants, ~600 genes were up- or downregulated, whereas in NL leaves #4 ~3000 genes were up- or downregulated. In contrast, in 15-week-old compared to 13-week-old upper canopy leaves #8 more genes were up- or downregulated in optimally N-supplied plants (~2000 genes) than in N-depleted plants (~750 genes). This opposing effect of N depletion on gene regulation was even more prominent among photosynthesis-related genes (PSGs). Between week 13 and 14 in leaves #4, 99 of 110 PSGs were downregulated in NL plants, but none in NO plants. In contrast, from weeks 13 to 16 in leaves #8 of NL plants only 11 PSGs were downregulated in comparison to 66 PSGs in NO plants. Different effects of N depletion in lower versus upper canopy leaves were also apparent in upregulation of autophagy genes and NAC transcription factors. More than half of the regulated NAC and WRKY transcription factor, autophagy and protease genes were specifically regulated in NL leaves #4 or NO leaves #8 and thus may contribute to differences in senescence and nutrient mobilization in these leaves. We suggest that in N-deficient plants the upper leaves retain their N resources longer than in amply fertilized plants and remobilize them only after shedding of the lower leaves.
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Claims 10
Claim · model-written summary
At week 12, optimal and deficient N plants were phenotypically and transcriptomically identical.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Material
- Mozarte0
- Outcome
- phenotypically and transcriptomically identicale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“In the spring oilseed rape (OSR) cultivar 'Mozart' grown under optimal N supply (NO) or mild N deficiency (NL) the transcriptome changes associated with progressing age until early senescence in developmentally old lower canopy leaves (leaf #4) and younger higher canopy leaves (leaf #8) were investigated. Twelve weeks old NO and NL plants appeared phenotypically and transcriptomically identical”
t0“Brassica napus”
Claim · model-written summary
N supply and leaf position altered transcript regulation: ~600 genes in NO.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- NOe0
- Outcome
- genes were up- or downregulatede0
- Value
- ~600 genese0
- Comparator
- 13-week-old plantse0
Not reportedMaterialDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In NO leaves #4 of 14-week-old compared to 13-week-old plants, ~600 genes were up- or downregulated”
t0“Brassica napus”
Claim · model-written summary
N supply and leaf position altered transcript regulation: ~3000 genes in NL.
- Taxon
- Brassica napusBrassica napust0
- Material
- Mozarte1
- Treatment
- NLe0
- Outcome
- genes were up- or downregulatede0
- Value
- ~3000 genese0
- Comparator
- NOe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in NL leaves #4 ~3000 genes were up- or downregulated”
e1“In the spring oilseed rape (OSR) cultivar 'Mozart' grown under optimal N supply (NO) or mild N deficiency (NL)”
t0“Brassica napus”
Claim · model-written summary
N supply and leaf position altered transcript regulation: ~2000 genes in optimally N-supplied plants.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- optimally N-supplied plantse0
- Outcome
- genes were up- or downregulatede0
- Value
- ~2000 genese0
- Comparator
- N-depleted plantse0
Not reportedMaterialDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in 15-week-old compared to 13-week-old upper canopy leaves #8 more genes were up- or downregulated in optimally N-supplied plants (~2000 genes) than in N-depleted plants (~750 genes)”
t0“Brassica napus”
Claim · model-written summary
N supply and leaf position altered transcript regulation: ~750 genes in N-depleted plants.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- N-depleted plantse0
- Outcome
- genes were up- or downregulatede0
- Value
- ~750 genese0
- Comparator
- optimally N-supplied plantse0
Not reportedMaterialDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in 15-week-old compared to 13-week-old upper canopy leaves #8 more genes were up- or downregulated in optimally N-supplied plants (~2000 genes) than in N-depleted plants (~750 genes)”
t0“Brassica napus”
Claim · model-written summary
N supply and leaf position altered transcript regulation: 99 of 110 in NL plants.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- NL plantse0
- Outcome
- PSGse0
- Direction
- downregulatede0
- Value
- 99 of 110e0
- Comparator
- NO plantse0
Not reportedMaterial
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Between week 13 and 14 in leaves #4, 99 of 110 PSGs were downregulated in NL plants, but none in NO plants”
t0“Brassica napus”
Claim · model-written summary
N supply and leaf position altered transcript regulation: 11 PSGs in NL plants.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- NL plantse0
- Outcome
- PSGse0
- Direction
- downregulatede0
- Value
- 11 PSGse0
- Comparator
- NO plantse0
Not reportedMaterial
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from weeks 13 to 16 in leaves #8 of NL plants only 11 PSGs were downregulated in comparison to 66 PSGs in NO plants”
t0“Brassica napus”
Claim · model-written summary
N supply and leaf position altered transcript regulation: 66 PSGs in NO plants.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- NO plantse0
- Outcome
- PSGse0
- Direction
- downregulatede0
- Value
- 66 PSGse0
- Comparator
- NL plantse0
Not reportedMaterial
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from weeks 13 to 16 in leaves #8 of NL plants only 11 PSGs were downregulated in comparison to 66 PSGs in NO plants”
t0“Brassica napus”
Claim · model-written summary
More than half of regulated transcription factor, autophagy and protease genes showed leaf and N specific regulation.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- specifically regulatede0
- Value
- More than halfe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More than half of the regulated NAC and WRKY transcription factor, autophagy and protease genes were specifically regulated in NL leaves #4 or NO leaves #8”
t0“Brassica napus”
Claim · model-written summary
Authors suggest upper leaves of N-deficient plants retain N longer and remobilize it after lower leaf shedding.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- N-deficient plantse0
- Outcome
- upper leaves retain their N resources longere0
- Qualifier
- only after shedding of the lower leavese0
- Comparator
- amply fertilized plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We suggest that in N-deficient plants the upper leaves retain their N resources longer than in amply fertilized plants and remobilize them only after shedding of the lower leaves.”
t0“Brassica napus”