Paper · 2017 · Global Journal Of Botanical Science

Improving In Vitro Somatic Embryos Production of Medjool and Khalas Date Palm Cultivars via Modification of Ammonium and Potassium Nitrate

Ibrahim A. Ibrahim; Awatef M. Badr-Elden; Hamdy A. Emara; et al.

7
claims
13
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by-nc

Abstract

Date palm is one of the most economically powerful fruit tree grown in Egypt. Although Egypt tops the list of date's product in the world. Egyptian date palm industry suffers from many constraints. Low-quality varieties and limited conventional propagation methods are the main obstacles. Extension of date palm plantations in various areas of Egypt and replanting trees to compensate loss due to diseases or human factors are reduced expected to lack adequate planting materials. The knowledge of the interplay between some fundamental medium parameters allows not only the optimization of the micropropagation system but also gives models to investigate and rationalize the process of induction and development of somatic embryogenesis itself. In vitro growth and development of produced embryos and plantlets has been improved by using of full MS salt strength. MS medium modification of some nutrients concentrations, i.e. NH4NO3(1237.5 mg/l) which recorded (13.20 and 9.60 embryos/explant for Medjool and Khalas respectively) at the end of three subculture. KNO3 which gave a higher score of mature somatic embryos (14.40 and 10.00 embryo/culture for Medjool and Khalas respectively) was obtained with 1425 mg/l KNO3 after three subcultures as compared to those obtained from any other treatment combination. The morphological response of somatic embryos production is controlled by some internal factors that fall under the influence of the genetic make-up of the plant and specialized for each genotype that is responsible for the production of somatic embryos (Medjool and Khalas cultivars). The genetic make-up is a decisive factor during somatic embryos production. There are differences between the two cultivars named, Medjool and Khalas. The produced somatic embryos at germination stage were transferred from the two experiments namely ammonium nitrate and potassium nitrate to multiplication and rooting stages for more in vitro growth for 12 weeks at three re-cultures. Individual shoots were cultured on modified MS basal medium in addition to IBA (1.0 mg/l), sucrose (30 g/l) and solidified with phyto-agar (8.0 g/l) for more in vitro growth and development. In vitro plantlets were transferred to acclimatization stage in plastic pots 5*18 cm diameter containing peatmoss, perlite and washed sand at equal volume. In vitro date palm plantlets produced from rooting stage grow well in the greenhouse during acclimatization stage without morphological abnormality. The growth and development of Medjool cultivar were better than Khalas during in vitro culture and ex vitro acclimatization.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Full-strength MS salts improved in vitro growth and development of embryos and plantlets.

Taxon
Date PalmPhoenix dactyliferat0
Treatment
full MS salt strengthe0
Outcome
growth and development of produced embryos and plantletse0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In vitro growth and development of produced embryos and plantlets has been improved by using of full MS salt strength”

✓ located verbatim in the source · abstract, characters 746–863 · show in text ↑
t0

“Date Palm”

✓ located verbatim in the source · title, characters 68–77 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

NH4NO3 at 1237.5 mg/l yielded 13.20 embryos per explant for Medjool.

Taxon
Date PalmPhoenix dactyliferat0
Material
Medjoole0
Treatment
NH4NO3(1237.5 mg/l)e0
Outcome
embryos/explante0
Value
13.20e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NH4NO3(1237.5 mg/l) which recorded (13.20 and 9.60 embryos/explant for Medjool and Khalas respectively)”

✓ located verbatim in the source · abstract, characters 927–1030 · show in text ↑
t0

“Date Palm”

✓ located verbatim in the source · title, characters 68–77 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

NH4NO3 at 1237.5 mg/l yielded 9.60 embryos per explant for Khalas.

Taxon
Date PalmPhoenix dactyliferat0
Material
Khalase0
Treatment
NH4NO3(1237.5 mg/l)e0
Outcome
embryos/explante0
Value
9.60e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NH4NO3(1237.5 mg/l) which recorded (13.20 and 9.60 embryos/explant for Medjool and Khalas respectively)”

✓ located verbatim in the source · abstract, characters 927–1030 · show in text ↑
t0

“Date Palm”

✓ located verbatim in the source · title, characters 68–77 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

KNO3 at 1425 mg/l produced 14.40 mature embryos per culture for Medjool.

Taxon
Date PalmPhoenix dactyliferat0
Material
Medjoole0
Treatment
1425 mg/l KNO3e0
Outcome
mature somatic embryose0
Direction
highere0
Value
14.40e0
Comparator
any other treatment combinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“KNO3 which gave a higher score of mature somatic embryos (14.40 and 10.00 embryo/culture for Medjool and Khalas respectively) was obtained with 1425 mg/l KNO3 after three subcultures as compared to those obtained from any other treatment combination”

✓ located verbatim in the source · abstract, characters 1063–1312 · show in text ↑
t0

“Date Palm”

✓ located verbatim in the source · title, characters 68–77 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

KNO3 at 1425 mg/l produced 10.00 mature embryos per culture for Khalas.

Taxon
Date PalmPhoenix dactyliferat0
Material
Khalase0
Treatment
1425 mg/l KNO3e0
Outcome
mature somatic embryose0
Direction
highere0
Value
10.00 embryo/culturee0
Comparator
any other treatment combinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“KNO3 which gave a higher score of mature somatic embryos (14.40 and 10.00 embryo/culture for Medjool and Khalas respectively) was obtained with 1425 mg/l KNO3 after three subcultures as compared to those obtained from any other treatment combination”

✓ located verbatim in the source · abstract, characters 1063–1312 · show in text ↑
t0

“Date Palm”

✓ located verbatim in the source · title, characters 68–77 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Date palm plantlets grew well in greenhouse acclimatization without morphological abnormalities.

Taxon
date palmPhoenix dactyliferae0
Organ / stage
plantletse0
Outcome
grow welle0
Setting
greenhousee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In vitro date palm plantlets produced from rooting stage grow well in the greenhouse during acclimatization stage without morphological abnormality”

✓ located verbatim in the source · abstract, characters 2333–2480 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Medjool grew and developed better than Khalas in vitro and during acclimatization.

Taxon
date palmPhoenix dactyliferat0
Material
Medjoole0
Outcome
growth and developmente0
Direction
bettere0
Comparator
Khalase0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The growth and development of Medjool cultivar were better than Khalas during in vitro culture and ex vitro acclimatization”

✓ located verbatim in the source · abstract, characters 2482–2605 · show in text ↑
t0

“Date Palm”

✓ located verbatim in the source · title, characters 68–77 · show in text ↑

potassium nitrate · growth · Phoenix dactylifera (date palm)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

salinity · growth · Phoenix dactylifera (date palm)Positive and negative results

17 papers: 1 positive, 16 negative or no effect

16 in the opposite direction

  1. negative
  2. negative
  3. negative

13 papers more in this group

See all 17 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.