Abstract
Fragaria chiloensis is a strawberry endemic from Chile with attractive white-pink fruit, pleasant aroma and taste. However, this fruit has a limited post-harvest period due to fast softening. Several transcription factors (TFs) are involved in the regulation of fruit ripening, and members of the NAC family have been implicated in cell wall remodeling. FcNAC1 was isolated from F. chiloensis fruit, coding a protein of 332 amino acid residues and displaying a characteristic NAC domain at the N terminus. FcNAC1 protein showed nuclear localization. An increase in transcript level was observed during ripening. A sequence of 1488 bp of FcNAC1 promoter was obtained. In silico analysis identified cis elements able to respond to some hormones and Secondary wall NAC binding elements (SNBE), and responding to auxin and ABA. A structural model of FcNAC1 provided evidence for interaction with DNA sequences containing SNBE, while a dual luciferase assay confirmed the transcriptional activation by FcNAC1 of the promoter of FcPL, a gene involved in cell wall remodeling in F. chiloensis fruit. The results suggest the participation of FcNAC1 during ripening development of strawberry fruit, by regulating pectin metabolism during softening.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
FcNAC1 protein localizes to the nucleus.
- Taxon
- Fragaria chiloensis
Fragaria chiloensist0 - Gene
- FcNAC1t0
- Outcome
- nuclear localizatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“FcNAC1 from Fragaria chiloensis fruit”
e1“FcNAC1 protein showed nuclear localization”
Claim · model-written summary
FcNAC1 transcript level increased during fruit ripening.
- Taxon
- Fragaria chiloensisFragaria chiloensist0
- Organ / stage
- fruitt0
- Gene
- FcNAC1t0
- Outcome
- transcript levele2
- Direction
- increasee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“FcNAC1 from Fragaria chiloensis fruit”
e2“An increase in transcript level was observed during ripening”
Claim · model-written summary
In silico analysis identified promoter cis elements responding to hormones, including auxin and ABA.
- Taxon
- Fragaria chiloensisFragaria chiloensist0
- Gene
- FcNAC1t0
- Outcome
- cis elementse3
- Qualifier
- responding to auxin and ABAe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“FcNAC1 from Fragaria chiloensis fruit”
e3“In silico analysis identified cis elements able to respond to some hormones and Secondary wall NAC binding elements (SNBE), and responding to auxin and ABA”
Claim · model-written summary
A structural model of FcNAC1 suggests interaction with DNA sequences containing SNBE.
- Taxon
- Fragaria chiloensisFragaria chiloensist0
- Gene
- FcNAC1t0
- Outcome
- interaction with DNA sequences containing SNBEe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“FcNAC1 from Fragaria chiloensis fruit”
e4“A structural model of FcNAC1 provided evidence for interaction with DNA sequences containing SNBE”
Claim · model-written summary
FcNAC1 transcriptionally activates the FcPL promoter in a dual luciferase assay.
- Taxon
- Fragaria chiloensisFragaria chiloensist0
- Gene
- FcPLe5
- Outcome
- transcriptional activatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“FcNAC1 from Fragaria chiloensis fruit”
e5“dual luciferase assay confirmed the transcriptional activation by FcNAC1 of the promoter of FcPL”
Claim · model-written summary
FcNAC1 likely participates in strawberry fruit ripening by regulating pectin metabolism during softening.
- Taxon
- Fragaria chiloensisFragaria chiloensist0
- Gene
- FcNAC1t0
- Outcome
- ripening developmente6
- Qualifier
- regulating pectin metabolisme6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“FcNAC1 from Fragaria chiloensis fruit”
e6“The results suggest the participation of FcNAC1 during ripening development of strawberry fruit, by regulating pectin metabolism during softening”