Abstract
In the present study, Fenugreek (Trigonella foenum graecum) seeds were surface sterilized and allowed to germinate on hormone free Murashige and Skoog's culture media for one week. Hypocotyls were cut into 0.5 mm length segments. Callus morphogenesis was achieved from the hypocotyls segments Using Murashige and Skoog's culture media supplemented with benzyl adenine and naphthalene acetic acid. The best callus biomass was obtained with 2 mg/l (BA) plus 1.5 mg/L (NAA) and the growth biomass was measured after 4 weeks. This concentration was then used to plot the growth curve of callus under both dark and light growth conditions. Calli obtained under light conditions exhibited a greenish yellow pigmentation, generally accumulated better biomass and their cells contained numerous and well formed discoid plastids. Calli obtained after 3, 6 and 9 weeks of growth were harvested, dried, and grinded into a fine powder and then extracted with ethanol 80%. Antibacterial activity of various calli extracts was checked by the disc-plate diffusion method and the results obtained have indicated that both hypocotyls and calli obtained from these explants exhibited activity against the tested bacteria and it was generally observed that calli were more potent than hypocotyls and that calli induced under light conditions were more potent in some cases than dark-induced calli in inhibiting the bacterial growth.
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Claims 7
Claim · model-written summary
The best callus biomass of fenugreek was obtained with 2 mg/l BA plus 1.5 mg/L NAA.
- Taxon
- Trigonella foenum graecume0
- Treatment
- 2 mg/l (BA) plus 1.5 mg/L (NAA)e1
- Outcome
- callus biomasse1
- Direction
- beste1
Not reportedComparator
Verbatim
e0“Fenugreek (Trigonella foenum graecum) seeds were surface sterilized”
e1“The best callus biomass was obtained with 2 mg/l (BA) plus 1.5 mg/L (NAA)”
Claim · model-written summary
Calli induced under light generally accumulated better biomass.
- Taxon
- Trigonella foenum graecume0
- Treatment
- light conditionse2
- Outcome
- biomasse2
- Direction
- bettere2
- Qualifier
- generallye2
Not reportedComparator
Verbatim
e0“Fenugreek (Trigonella foenum graecum) seeds were surface sterilized”
e2“Calli obtained under light conditions exhibited a greenish yellow pigmentation, generally accumulated better biomass and their cells contained numerous and well formed discoid plastids”
Claim · model-written summary
Light-induced calli exhibited a greenish yellow pigmentation.
Verbatim
e0“Fenugreek (Trigonella foenum graecum) seeds were surface sterilized”
e2“Calli obtained under light conditions exhibited a greenish yellow pigmentation, generally accumulated better biomass and their cells contained numerous and well formed discoid plastids”
Claim · model-written summary
Cells of light-induced calli contained numerous well formed discoid plastids.
Verbatim
e0“Fenugreek (Trigonella foenum graecum) seeds were surface sterilized”
e2“Calli obtained under light conditions exhibited a greenish yellow pigmentation, generally accumulated better biomass and their cells contained numerous and well formed discoid plastids”
Claim · model-written summary
Both hypocotyl and callus extracts of fenugreek were active against the tested bacteria.
Verbatim
e0“Fenugreek (Trigonella foenum graecum) seeds were surface sterilized”
e3“both hypocotyls and calli obtained from these explants exhibited activity against the tested bacteria”
Claim · model-written summary
Callus extracts were more potent than hypocotyl extracts against the tested bacteria.
Verbatim
e0“Fenugreek (Trigonella foenum graecum) seeds were surface sterilized”
e4“calli were more potent than hypocotyls”
Claim · model-written summary
Calli induced under light were more potent than dark-induced calli in inhibiting bacterial growth in some cases.
Verbatim
e0“Fenugreek (Trigonella foenum graecum) seeds were surface sterilized”
e5“calli induced under light conditions were more potent in some cases than dark-induced calli in inhibiting the bacterial growth”
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See all 5 papers →1-naphthaleneacetic acid · growth · Trigonella foenum-graecum (fenugreek)Results agree
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See all 7 papers →light · growth · Trigonella foenum-graecum (fenugreek)Positive and negative results
2 in the opposite direction
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.