Paper · 2018 · The Pakistan Journal of Agricultural Sciences

INDUCING DROUGHT TOLERANCE IN WHEAT THROUGH COMBINED USE OF L-TRYPTOPHAN AND Pseudomonas fluorescens

Moazzam Jamil

10
claims
20
verbatim quotes
35
citations
2
research areas
—
Not open access

Abstract

Auxins, as phytohormones and osmo-protectant, play important role to alleviate the adverse effect of drought stress on plant growth.Some bacteria equipped with ACC-deaminase activity along with some other growth promoting traits are helpful in improving plant growth under stresses.The efficiency of these bacteria is further improved when inoculation is supplemented with exogenous plant growth regulators.The combined use of these bacteria with auxins may be more effective under stressed conditions.A pot experiment was conducted to evaluate the efficacy of Pseudomonas fluorescens containing ACC-deaminase in combination with L-tryptophan for reducing drought stress in wheat.The P. fluorescens and L-tryptophan (@ 25 ppm) were applied, solely and in combination, on wheat by inducing drought stress at different growth stages.The foliar application of Ltryptophan was started after 15 days of germination and was repeated every week up to 2 weeks after grain filling stage.The results showed significant reduction in all parameters under drought.However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.Consequently, it may be concluded that combined use of Ltryptophan @ 25 ppm and P. fluorescens is an effective approach to improve wheat productivity under water limited conditions but it should be evaluated extensively under field studies before its recommendation to the farmers.

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Claims 10

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved plant height by 45.65% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
plant heighte0
Direction
improvede0
Value
45.65%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved root length by 81% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
root lengthe0
Direction
improvede0
Value
81%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved chlorophyll-a by 94% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
chlorophyll-ae0
Direction
improvede0
Value
94%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved chlorophyll-b by 72% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
chlorophyll-be0
Direction
improvede0
Value
72%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved carotenoid contents by 98% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
carotenoid contentse0
Direction
improvede0
Value
98%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved relative water contents by 33% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
relative water contentse0
Direction
improvede0
Value
33%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved Na + /K + ratio in leaves by 49% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
Na + /K + ratio in leavese0
Direction
improvede0
Value
49%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved Na + /K + ratio in by 43% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
Na + /K + ratio ine0
Direction
improvede0
Value
43%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved spike length by 64% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
spike lengthe0
Direction
improvede0
Value
64%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined L-tryptophan and P. fluorescens improved 1000 grains weight by 75% under drought.

Treatment
combined application of L-tryptophan @ 25 ppm and P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0other
Outcome
1000 grains weighte0
Direction
improvede0
Value
75%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, combined application of L-tryptophan @ 25 ppm and P. fluorescens significantly improved plant height (45.65%), root length (81%), chlorophyll-a (94%), chlorophyll-b (72%), carotenoid contents (98%), relative water contents (33%), Na + /K + ratio in leaves (49%) and grains (43%), spike length (64%) and 1000 grains weight (75%) under drought stress.”

✓ located verbatim in the source · abstract, characters 1051–1409 · show in text ↑
t0

“WHEAT”

✓ located verbatim in the source · title, characters 30–35 · show in text ↑

L-tryptophan · growth · Triticum aestivum (wheat)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

L-tryptophan · yield · Triticum aestivum (wheat)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Pseudomonas fluorescens · yield · Triticum aestivum (wheat)Results agree

9 papers: 9 positive, 0 negative or no effect

8 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

Pseudomonas fluorescens · growth · Triticum aestivum (wheat)Results agree

15 papers: 15 positive, 0 negative or no effect

14 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

11 papers more in this group

See all 15 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.